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EP0354441A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Method for the colorimetric determination of analyte using enzymatic oxydation

Assignee: Roche Diagnostics GmbH
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Filing: 1989-08-01
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Granted: —
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Expires: 2009-08-01
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Abstract

Abstract Translated from German Die Erfindung betrifft ein Verfahren zur kolorimetrischen Bestimmung eines Analyten mittels enzymatischer Oxidation des Analyten in Gegenwart eines Elektronenakzeptors und Bestimmung des reduzierten Elektronenakzeptors durch Farbbildung als Maß für die Menge des Analyten, dadurch gekennzeichnet, daß der Analyt mit einer entsprechenden Oxidoreductase in Anwesenheit einer Substanz aus der Gruppe der Verbindungen mit Stickstoff in einer Oxidationsstufe zwischen +1 und -1 als direktem Elekteonenakzeptor oxidiert wird sowie ein hierfür geeignetes Mittel. The invention relates to a method for the colorimetric determination of an analyte by means of enzymatic oxidation of the analyte in the presence of an electron acceptor and determination of the reduced electron acceptor by color formation as a measure of the amount of the analyte, characterized in that the analyte with a corresponding oxidoreductase in the presence of a substance from the Group of compounds is oxidized with nitrogen in an oxidation state between +1 and -1 as a direct electron acceptor and a suitable agent for this.

Inventors

  • 👤 Joachim Dr. Hoenes

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-01 01:24
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; H; H01; H01R; H01R13/00; H01R13/73; H05; H05K; H05K7/00; H05K7/02; H05K7/12; H01R13/46

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