EP0352894A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Production of cosmetic or pharmaceutical composition
Assignee: Unilever PLC
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Filing: 1989-06-15
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Granted: —
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Expires: 2009-06-15
Abstract
Abstract Dermal papillae cells are immortalized and cultured to produce hair growth promoter. The hair growth promoter is then collected and used to prepare compositions for application to the human skin.
Inventors
- 👤 Susan Ann Bayley
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 22:34
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; A; A61; A61K; A61K8/00; A61K8/18; A61K8/30; A61K8/49; A61K8/494; A61K8/4953; A61Q; A61Q7/00; C07; C07K; C07K14/00; C07K14/005; C12N5/00; C12N5/06; C12N5/0602; C12N5/0625; C12N5/0627; A61K2800/00; A61K2800/80; A61K2800/86; C12N2510/00; C12N2510/04; C12N2710/00; C12N2710/00011; C12N2710/22011; C12N2710/22022; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/13011; C12N2740/13041; C12N2740/13043
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
17 words
Dermal papillae cells transfected by incorporation of an immortalizing gene into the genetic information of the cells.
Claim 2
Dependent Claim
17 words
Dermal papillae cells transfected by incorporation of an immortalizing gene into the genetic information of the cells.
Claim 3
Dependent Claim
14 words
Cells according to claim 1 wherein the immortalizing gene is from an oncogenic virus.
Claim 4
Dependent Claim
14 words
Cells according to claim 2 wherein the immortalizing gene is from a polyoma virus.
Claim 5
Independent Claim
29 words
A method of making immortalized dermal papillae cells which comprises transfecting the cells by recombinant DNA technology to incorporate an immortalizing gene into the genetic information of the cells.
Claim 6
Independent Claim
29 words
A method of making immortalized dermal papillae cells which comprises transfecting the cells by recombinant DNA technology to incorporate an immortalizing gene into the genetic information of the cells.
Claim 7
Dependent Claim
36 words
A method according to claim 4 which comprises collecting and culturing dermal papillae cells, transfecting the cultured cells with recombinant vectors carrying an immortalizing gene, and selecting cells which have been transfected with the immortalizing gene.
Claim 8
Dependent Claim
107 words
A method according to claim 5 which comprises the steps of: a) dissecting dermal papillae explants out of hair follicles; b) maintaining and/or manipulating the explants in tissue culture so that individual dermal papillae cells migrate out of the explants; c) maintaining and/or manipulating the dermal papillae cells in tissue culture to give subconfluent monolayers; d) transfecting the cultured dermal papillae cells with one or more recombinant vectors carrying an immortalizing gene and a gene aiding selection of the transformants; e) maintaining and/or manipulating the dermal papillae cells in tissue culture; f) selecting dermal papillae cells which have been transfected by the incorporation of an immortalizing gene.
Claim 9
Dependent Claim
34 words
A method according to claim 6 further comprising the steps of: g) maintaining and/or manipulating the selected transfected immortalized dermal papillae cells in tissue culture; h) cryopreserving batches of the immortalized dermal papillae cells.
Claim 10
Dependent Claim
16 words
A method according to claim 6 or claim 7 wherein step (c) includes passaging the cells.
Claim 11
Independent Claim
21 words
A method according to any one of claims 4 to 8 wherein the immortalizing gene is provided by an oncogenic virus.
Claim 12
Dependent Claim
16 words
A method according to claim 9 wherein the immortalizing gene is provided by a polyoma virus.
Claim 13
Independent Claim
18 words
A method according to any one of claims 4 to 10 wherein the recombinant vector is a retrovirus.
Claim 14
Dependent Claim
12 words
Production of hair growth promoter by means of immortalizing dermal papillae cells.
Claim 15
Dependent Claim
12 words
Production of hair growth promoter by means of immortalizing dermal papillae cells.
Claim 16
Independent Claim
50 words
A method of making hair growth promoter comprising utilising dermal papillae cells according to any one of claims 1 to 3 or produced by a method according to any one of claims 4 to 11, and culturing the said cells under conditions for the cells to produce hair growth promoter.
Claim 17
Dependent Claim
34 words
A method according to claim 13 which comprises the steps of: a) maintaining immortalized dermal papillae cells in tissue culture; b) maintaining the tissue culture conditions appropriate for the production of hair growth promoter.
Claim 18
Dependent Claim
21 words
A method according to claim 13 or claim 14 further comprising recovering the hair growth promoter from the tissue culture supernatant.
Claim 19
Dependent Claim
19 words
Culture supernatant containing hair growth promoter produced according to the methods of any one of claims 13 to 15.
Claim 20
Dependent Claim
51 words
A composition containing hair growth promoter produced according to any one of claims 13 to 15, and which is sufficient to increase hair growth in the rat, when applied thereto, by at least 10% more than that obtainable using a control composition from which the hair growth promoter has been omitted.
Claim 21
Dependent Claim
51 words
A composition according to claim 17 wherein at least a part of the hair growth promoter is characterised by: i) being substantially proteinaceous; ii) having an apparent molecular weight of at least 500 Daltons; and iii) possessing the ability to initiate DNA synthesis in a culture of serum-starved NIH 3T3 cells.
Claim 22
Dependent Claim
30 words
A composition according to claim 17 or claim 18 in which the hair growth promoter forms from 1 x 10⁻⁶% to 99% by weight, preferably 1 x 10⁻³% to 90%.
Claim 23
Dependent Claim
22 words
A composition according to any of claims 17 to 19 which comprises a cosmetically acceptable vehicle in addition to the culture supernatant.
Claim 24
Dependent Claim
28 words
A composition according to any of claims 17 to 20 which additionally comprises an activity enhancer chosen from other hair growth stimulants, protein stabilising agents and penetration enhancers.
Claim 25
Dependent Claim
24 words
A composition according to claim 21, in which the other hair growth stimulant is chosen from minoxidil, minoxidil glucuronides, minoxidil sulphates or mixtures thereof.
Claim 26
Dependent Claim
27 words
A composition according to claim 21 or claim 22 in which the protein stabilising agent is chosen from glycerol, ethylenediaminetetraacetic acid, cysteine, α₂-macroglobulin, serum or mixtures thereof.
Claim 27
Dependent Claim
16 words
A method of inducing cosmetic hair growth which comprises administering a composition according to claim 20.
References Cited (Backward Citations)
No citation records indexed.
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