Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
17 words
A non-human mammalian cell line charaterized in that the cell line expresses CEA family cell surface antigens.
Claim 2
Dependent Claim
17 words
A non-human mammalian cell line charaterized in that the cell line expresses CEA family cell surface antigens.
Claim 3
Dependent Claim
26 words
A cell line according to claim 1 characterized in that it is a primary transfectant prepared by gene transfection of human DNA into non-human mammalian cells.
Claim 4
Dependent Claim
25 words
A cell line according to claim 2, wherein the human DNA is LoVo cell genomic DNA and the non-human mammalian cells are mouse Ltk- cells.
Claim 5
Dependent Claim
27 words
A cell line according to claim 1, characterized in that it is a secondary transfectant prepared by gene transfection of primary transfectant DNA into non-human mammalian cells.
Claim 6
Dependent Claim
44 words
A cell line according to claim 4, wherein the primary transfectant DNA is from a transfectant prepared by gene transfection of LoVo cell genomic DNA into mouse Ltk- cells and wherein the non-human mammalian cells of the secondary transfectant are also mouse Ltk- cells.
Claim 7
Dependent Claim
42 words
A cell line according to claim 4, characterized in that it is a substantially homogeneous high expression cloned cell line derived from secondary transfectants, which cloned cell line expresses about twenty fold or greater more human CEA family antigen than secondary transfectants.
Claim 8
Dependent Claim
52 words
A cell line according to claim 1, which is prepared by the transfection of a cDNA coding a CEA family antigen which has been cloned into a cDNA expression vector in non-human mammalian cells, preferably wherein said cDNA is selected from the group consisting of CEA-(b), CEA-(c), CEA-(d), CEA-(e), CEA-(f) and CEA-(g).
Claim 9
Dependent Claim
18 words
A cell line according to claim 7, designated as CRL 9731, CRL 9730, CRL 9733, or CRL 9732.
Claim 10
Dependent Claim
16 words
A cell line according to claims 1-8, wherein said cell line is a mouse cell line.
Claim 11
Dependent Claim
92 words
A monoclonal antibody to a CEA family antigen, said monoclonal antibody prepared by a method comprising the steps of (A) immunizing a competent host animal with (i) non-human mammalian cells from a cell line according to any of claims 1-9 which express said antigen or (ii) said antigen purified from said cells, (B) fusing lymphocytes isolated from the host animal which produce antibodies to said receptor with myeloma cells to form hybridomas, (C) isolating a hybridoma clone which secretes a monoclonal antibody to said antigen, and (D) harvesting the secreted monoclonal antibody.