Patent US655707A successfully refreshed from Google Patents.
EP0339780A2
Expired / Lapsed
EP
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Capillary detector cartridge for electrophoresis
Assignee: Beckman Coulter Inc
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Filing: 1989-03-14
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Granted: —
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Expires: 2009-03-14
Abstract
Abstract A capillary for electrophoresis is disposed in a cartridge body. The cartridge body is cooled and provides a serpentine path for the capillary. The capillary is disposed over the serpentine path and protrudes vertically downward from the bottom of the cartridge at two remote spaced apart locations at the bottom of the cartridge. These two remote spaced apart locations dispose the protruding capillary ends at the same elevation to restrict flow within the capillary to flow induced by electrical force or fluid pressure injection. These distal ends are sealed about their periphery with respect to the cartridge where they exit the cartridge. The distal ends protrude downwardly a sufficient distance from the bottom of the cartridge body to be able to become immersed in an electrolyte for filling the capillary (preferably at a high pressure on one end of the capillary -- about 10 psi), a sample for charging the capillary (at a low measure pressure at one end of the capillary -- about 1 psi) or an electrolyte at an electrical connection for causing electrophoresis in the capillary (with the same pressure at both ends of the capillary). One portion of the capillary adjacent to an end is registered to detector components defined by the cartridge. In one preferred embodiment the capillary is placed within a hollow cartridge body, spiral wound about a mandrel and cooled by a coolant, which can be forced circulated to dissipate by convection heat generated during the electrophoresis. An alternate construction includes a boron nitride block having a serpentine depression. A capillary is threaded to the required length in the serpentine depression, insulated at points of crossover to prevent undesired arcing, and pasted to the block with water soluble boron nitride paste. In both cases, the cartridge provides an immediately interchangeable site for electrophoresis which can conveniently be utilized for automatic electrolyte and sample injection, automated electrophoresis and automated detection without special filling connected to the capillary. Interchanging of the cartridge permits rapid change of length, detection mode and capillaries merely by the substitution of cartridges.
Inventors
- 👤 Victor Paul Burolla
- 👤 Ian Keith Glasgow
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 10:25
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
223 words
A cartridge for electrophoresis comprising in combination: a cartridge body having a bottom surface; a capillary having a predetermined length and two distal ends for placement in said cartridge body; said cartridge body defining at the bottom surface thereof first and second space apart exits for disposing the distal ends of said capillaries in depending relation from said body through the bottom of said body a distance below the bottom surface of said body to enable penetration of the capillary ends into fluid baths for the communication of said capillary to said fluid baths; a serpentine path for winding a capillary defined within said block of material between said first and second spaced apart exit paths; said capillary wound to said serpentine path between the distal ends thereof; said capillary as wound to said serpentine paths having the distal ends of said capillary depending from the bottom of said cartridge body; means for registering said capillary with said detector means for enabling detection of capillary electrophoresis through said capillary as disposed within said block of material interior of said cartridge body; and, means for sealing the periphery of said capillary to said cartridge body for permitting a seal made around said capillary at said cartridge body to permit fluid pressure to be communicated against said cartridge bottom to be communicated through said capillary.
Claim 2
Dependent Claim
223 words
A cartridge for electrophoresis comprising in combination: a cartridge body having a bottom surface; a capillary having a predetermined length and two distal ends for placement in said cartridge body; said cartridge body defining at the bottom surface thereof first and second space apart exits for disposing the distal ends of said capillaries in depending relation from said body through the bottom of said body a distance below the bottom surface of said body to enable penetration of the capillary ends into fluid baths for the communication of said capillary to said fluid baths; a serpentine path for winding a capillary defined within said block of material between said first and second spaced apart exit paths; said capillary wound to said serpentine path between the distal ends thereof; said capillary as wound to said serpentine paths having the distal ends of said capillary depending from the bottom of said cartridge body; means for registering said capillary with said detector means for enabling detection of capillary electrophoresis through said capillary as disposed within said block of material interior of said cartridge body; and, means for sealing the periphery of said capillary to said cartridge body for permitting a seal made around said capillary at said cartridge body to permit fluid pressure to be communicated against said cartridge bottom to be communicated through said capillary.
Claim 3
Dependent Claim
22 words
The invention of claim 1 and wherein said cartridge body defines a hollow interior coolant is confined within said hollow cartridge interior.
Claim 4
Dependent Claim
26 words
The invention of claim 2 and wherein said cartridge defines a coolant inlet and a coolant outlet for the forced circulation of coolant through said cartridge.
Claim 5
Dependent Claim
45 words
The invention of claim 1 and wherein said cartridge is solid; and said serpentine path is defined in a groove configured from one side edge of said solid cartridge; and, thermally conductive material fills said serpentine path and confines said capillary to said serpentine path.
Claim 6
Dependent Claim
20 words
The invention of claim 2 and wherein said serpentine path includes a cylindrical mandrel in the interior of said cartridge.
Claim 7
Dependent Claim
32 words
The invention of claim 4 and wherein one portion of said serpentine channels crosses another portion of said serpentine channel, one of said channels being deeper than the other of said channel.
Claim 8
Dependent Claim
181 words
A cartridge for capillary electrophoresis, said cartridge for maintaining a capillary having two spaced apart distal ends therein, said cartridge comprising in combination: a cartridge body having a bottom surface; a serpentine path for winding said capillary defined within said cartridge body; first and second exit paths defined from said serpentine path to and through the bottom surface of said cartridge body for disposing said capillary at said distal end with each distal end in depending relationship from said block of material at each exit a distance below said bottom surface of said cartridge to enable penetration of a capillary wound in said serpentine path into a fluid bath for communication of said capillary to said fluid bath; a detector aperture defined in said block of material adjacent to one of said exit paths; means for registering said capillary to said detector aperture for enabling detection of capillary electrophoresis through said capillary; and, means for sealing said capillary at said exit paths whereby a seal made to said bottom of said cartridge around said capillary communicates fluid pressure to said capillary.
Claim 9
Dependent Claim
181 words
A cartridge for capillary electrophoresis, said cartridge for maintaining a capillary having two spaced apart distal ends therein, said cartridge comprising in combination: a cartridge body having a bottom surface; a serpentine path for winding said capillary defined within said cartridge body; first and second exit paths defined from said serpentine path to and through the bottom surface of said cartridge body for disposing said capillary at said distal end with each distal end in depending relationship from said block of material at each exit a distance below said bottom surface of said cartridge to enable penetration of a capillary wound in said serpentine path into a fluid bath for communication of said capillary to said fluid bath; a detector aperture defined in said block of material adjacent to one of said exit paths; means for registering said capillary to said detector aperture for enabling detection of capillary electrophoresis through said capillary; and, means for sealing said capillary at said exit paths whereby a seal made to said bottom of said cartridge around said capillary communicates fluid pressure to said capillary.
Claim 10
Dependent Claim
21 words
The invention of claim 7 and wherein said cartridge is fabricated with the channel configured from one side of said block.
Claim 11
Dependent Claim
14 words
The invention of claim 8 and wherein said block is configured from boron nitride.
Claim 12
Dependent Claim
28 words
The invention of claim 7 and wherein said body is hollow for accommodating coolant and has a cylindrical mandrel therein for winding said cartridge in a spiral path.
Claim 13
Dependent Claim
54 words
The invention of claim 7 and wherein said block is hollow; first and second conduits, each said conduit communicated to the hollow interior of said cartridge at the upper end and extending through the bottom of said cartridge at the lower end whereby said conduits can be used to fill said cartridge with coolant.
Claim 14
Dependent Claim
23 words
The invention of claim 7 and wherein said serpentine path includes a mandrel for the winding of said capillary interior of said cartridge.
Claim 15
Dependent Claim
33 words
The invention of claim 7 and wherein said means for sealing said capillary to said cartridge includes an aperture intersecting said exit path, said aperture permitting the introduction of sealant around said capillary.
Claim 16
Dependent Claim
159 words
Apparatus for electrophoresis in a capillary supported in a cartridge body, said apparatus comprising in combination; a capillary having two distal ends; a cartridge body having a bottom surface; a serpentine path defined in said cartridge body; first and second spaced apart exit paths defined from said serpentine path to and through the bottom surface of said cartridge body for disposing said capillary in depending relationship from said block of material a distance below said bottom surface of said cartridge to enable penetration of a capillary wound to said serpentine path into a fluid bath; a detector means defined in said block of material adjacent to one of said exit paths; means for registering said capillary to said detector means for enabling the detection of capillary electrophoresis through said block of material from the exterior of said cartridge; means for leveling said cartridge with respect to said detector means for enabling said cartridge bottom to be maintained substantially level.
Claim 17
Dependent Claim
159 words
Apparatus for electrophoresis in a capillary supported in a cartridge body, said apparatus comprising in combination; a capillary having two distal ends; a cartridge body having a bottom surface; a serpentine path defined in said cartridge body; first and second spaced apart exit paths defined from said serpentine path to and through the bottom surface of said cartridge body for disposing said capillary in depending relationship from said block of material a distance below said bottom surface of said cartridge to enable penetration of a capillary wound to said serpentine path into a fluid bath; a detector means defined in said block of material adjacent to one of said exit paths; means for registering said capillary to said detector means for enabling the detection of capillary electrophoresis through said block of material from the exterior of said cartridge; means for leveling said cartridge with respect to said detector means for enabling said cartridge bottom to be maintained substantially level.
Claim 18
Dependent Claim
15 words
The invention of claim 11 and wherein said cartridge body is hollow and contains coolant.
Claim 19
Dependent Claim
11 words
The invention of claim 11 and wherein said block is solid.
References Cited (Backward Citations)
No citation records indexed.
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