EP0339607A2
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EP
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Composition for repair of cartilage and bone and method for their preparation as skeletal tissue implant
Assignee: Individual
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Filing: 1989-04-26
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Granted: —
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Expires: 2009-04-26
Abstract
Abstract A composition for the regeneration of articular cartilage and bones by implantation comprising cells expressing a chondrocyte phenotype (80 x 10⁶ cells/ml), namely bone marrow derived chondrocytes or osteoblasts of autologous or homologous origin, or homologous committed chondrocytes, or autologous or homologous muscle fibroblast derived chondrocytes or any other progenital cells from mesenchymal origin, which are embedded in a biodegradable, biocompatible, biological resorbable immobilization vehicle (BRIV) adhesive glue consisting of fibrinogen, thrombin, CaCl₂, protease inhibitor, and at least 10%, preferably 15-30%, serum, and a process for the preparation of such composition as skeletal tissue implant. A portion of the implant may comprise a bone segment or a bone substitute.
Inventors
- 👤 Samuel Dr. Itay
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 21:01
CPC Classifications
A; A61; A61L; A61L24/00; A61L24/0005; A61F; A61F2/00; A61F2/02; A61F2/28; A61F2/30; A61F2/30756; A61K; A61K35/00; A61K35/12; A61K35/28; A61K35/34; A61L24/04; A61L24/10; A61L24/106; A61F2002/30001; A61F2002/30003; A61F2002/3006; A61F2002/30062; A61F2210/00; A61F2210/0004; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/838; Y10S530/84
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
66 words
A composition for the regeneration of articular cartilage and bones by implantation comprising, bone marrow derived chondrocytes or osteoblasts of autologous or homologous origin, or homologous commited chondrocytes, or autologous or homologous muscle fibroblast derived chondrocytes or any other progenital cells from mesenchymal origin and a biodegradable, biocompatible, biological resorbable immobilization vehicle (BRIV) adhesive glue consisting of fibrinogen, thrombin, CaCl₂, protease inhibitor, and about 15-30% serum.
Claim 2
Dependent Claim
66 words
A composition for the regeneration of articular cartilage and bones by implantation comprising, bone marrow derived chondrocytes or osteoblasts of autologous or homologous origin, or homologous commited chondrocytes, or autologous or homologous muscle fibroblast derived chondrocytes or any other progenital cells from mesenchymal origin and a biodegradable, biocompatible, biological resorbable immobilization vehicle (BRIV) adhesive glue consisting of fibrinogen, thrombin, CaCl₂, protease inhibitor, and about 15-30% serum.
Claim 3
Dependent Claim
14 words
The composition according to claim 1 containing from 80-160 x 10⁶ cells/ml of BRIV.
Claim 4
Dependent Claim
32 words
The composition according to claim 1, wherein said 15-30% serum comprises fetal calf serum or umbilical cord serum from the second trimester or horse serum or any combination of the such serums.
Claim 5
Dependent Claim
42 words
The composition according to claim 1, wherein the cells are of bone marrow derived chondrocytes at a concentration of 120 x 10⁶ cells/ml of BRIV that can be induced in culture to proliferate and be converted to express chondrogenic or osteogenic phenotypes.
Claim 6
Dependent Claim
42 words
The composition according to claim 1, wherein the cells are of muscle fibroblast derived chondrocytes at a concentration of 120 x 10⁶ cells/ml of BRIV that can be induced in culture to proliferate and be converted to express chondrogenic or osteogenic phenotypes.
Claim 7
Dependent Claim
25 words
The composition of claim 1, wherein the protease inhibitor is selected from the group consisting of polysaccharides, plasma protease, synthetic and natural non-plasma protease inhibitors.
Claim 8
Independent Claim
46 words
A method of preparing a skeletal tissue implant comprising the steps of: a. purification, proliferation and manipulation of a cell population expressing a specific chondrogenic or osteogenic phenotype; and b. embedding the cells in a biological resorbable immobilization vehicle (BRIV) comprising at least 10%, preferably 15-30%,serum.
Claim 9
Independent Claim
46 words
A method of preparing a skeletal tissue implant comprising the steps of: a. purification, proliferation and manipulation of a cell population expressing a specific chondrogenic or osteogenic phenotype; and b. embedding the cells in a biological resorbable immobilization vehicle (BRIV) comprising at least 10%, preferably 15-30%,serum.
Claim 10
Dependent Claim
38 words
The method as set forth in claim 7 above, wherein the proliferating cells are selected from the group consisting of bone marrow stroma cells, embryonal committed chondrocytes, or chondrocytes derived from muscle fibroblasts and any undifferentiated mesenchymal cells.
Claim 11
Dependent Claim
48 words
The method as set forth in claim 8 above, wherein the concentration of the cells is between 80 x 10⁶ and 160 x 10⁶ cells/ml of BRIV, wherein the BRIV includes 100-150 mg/ml fibrinogen and 60-90 units/ml thrombin, and wherein the BRIV further includes about 2,000 units/ml aprotonin.
Claim 12
Dependent Claim
23 words
The method as set forth in claim 7 above, wherein the cells are embedded in a BRIV in a suitably shaped bone substitute.
Claim 13
Independent Claim
103 words
The method as set forth in one of claims 7 to 10, comprising the steps of: a. culturing a proliferating cell population comprising autologous bone marrow stroma cells; b. manipulating them in suspension above soft agar at a concentration in excess of 2 x 10⁶ cell/ml of medium for at least five days; c. harvesting the cells; and d. embedding the cells at a concentration in excess of 80 x 10⁶ cells/ml of a biological resorbable immobilization vehicle (BRIV) including in excess of 10% serum, 100 mg/ml fibrinogen, and 60 units/ml thrombin in an excess of 40mM CaCl₂ and 2,000 units of aprotonin.
Claim 14
Independent Claim
109 words
The method as set forth in one of claims 7 to 10, comprising the steps of: a. culturing a proliferating cell population comprising autologous muscle fibroblast derived chrondocytes; b. manipulating the cells in suspension and culturing the cells above soft agar at a concentration in excess of 3 x 10⁶ cells/ml of medium for at least 3 days; c. harvesting the cells; and d. embedding the cells at a concentration in excess of 80 x 10⁶ cells/ml of a biological resorbable immobilization vehicle (BRIV), the BRIV comprising in excess of 10% serum, 100 mg/ml fibrinogen, 60 units/ml thrombin in an excess of 40mM CaCl₂, and 2000 units of aprotonin.
Claim 15
Dependent Claim
27 words
The method as set forth in claim 11 or 12 above, wherein the BRIV includes 15-30% serum and 60 units/ml thrombin in an excess of 60mM CaCl₂.
Claim 16
Independent Claim
42 words
The method as set forth in one of claims 11 to 13 above, wherein the biological implant is stored under low temperature conditions after preparation with a preservative medium containing 90% F.C.S. and 10% DMSO, and wherein there is about 20% serum.
Claim 17
Independent Claim
43 words
The method as set forth in one of claims 11 to 13 above, wherein the harvested cells are stored under low temperature conditions after preparation with a preservative medium containing 90% F.C.S. and 10% DMSO, and embedded at a later date in BRIV.
Claim 18
Dependent Claim
29 words
The method according to claim 11, wherein the serum is selected from the group consisting of fetal calf serum, umbilical cord serum from the second trimester, and horse serum.
References Cited (Backward Citations)
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