EP0336483A1
Expired / Lapsed
EP
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A process and device for the separation of a body fluid from particulate materials in said fluid and testing kit for said separation and analysis of the body fluid.
Assignee: Primecare BV
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Filing: 1989-03-23
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Granted: —
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Expires: 2009-03-23
Abstract
Abstract A process and device for the separation of a body fluid from particulate materials in said fluid to be used for a chemical analysis of the components present therein. Said process and device are in particular suitable for the separation of plasma from whole blood. Furthermore the present invention relates to a testing kit for the above mentioned separation and analysis purposes. Because of the simplicity of the present process and device as well as the testing kit a routine examination may be carried out not only in clinical laboratories but also by general practitioners.
Inventors
- 👤 Dirk Marinus Koenhen
- 👤 Johan Jan Scharstuhl
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 20:02
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/491; B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5023; G01N33/50; G01N33/52; G01N33/528; B01L2300/00; B01L2300/04; B01L2300/041; B01L2300/043; B01L2300/06; B01L2300/0681; B01L2300/08; B01L2300/0809; B01L2400/00; B01L2400/04; B01L2400/0403; B01L2400/0406; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375; Y10T436/255
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
143 words
A process for the separation of a body fluid from particulate materials in said fluid, in particular plasma from whole blood, by contacting a small amount of said body fluid respectively whole blood with a membrane or composite of membranes, wherein the body fluid respectively the whole blood penetrates into the membrane or composite of membranes, wherein the particulate materials and non-penetrated body fluid respectively whole blood penetrates, whereas the penetrated fluid respectively plasma is used for analysis purposes, characterized in that one or more hydrophilic microporous membranes, at least one of which (the collector membrane) has a defined pore volume, is or are used, wherein the absorbed body fluid, freed from the particulate materials if any and having a defined amount is used for a semi-quantitative or quantitative, dry or wet chemical analysis for the determination of one or more components thereof.
Claim 2
Dependent Claim
143 words
A process for the separation of a body fluid from particulate materials in said fluid, in particular plasma from whole blood, by contacting a small amount of said body fluid respectively whole blood with a membrane or composite of membranes, wherein the body fluid respectively the whole blood penetrates into the membrane or composite of membranes, wherein the particulate materials and non-penetrated body fluid respectively whole blood penetrates, whereas the penetrated fluid respectively plasma is used for analysis purposes, characterized in that one or more hydrophilic microporous membranes, at least one of which (the collector membrane) has a defined pore volume, is or are used, wherein the absorbed body fluid, freed from the particulate materials if any and having a defined amount is used for a semi-quantitative or quantitative, dry or wet chemical analysis for the determination of one or more components thereof.
Claim 3
Dependent Claim
37 words
The process according to claim 1, characterized in that a hydrophilic microporous membrane is used, which essentially consists of a hydrophobic polymer and a hydrophilic polymer, which hydrophilic polymer is fixed in or on the polymer matrix.
Claim 4
Dependent Claim
30 words
The process accordig to claim 2, characterized in that a membrane is used, wherein the hydrophobic polymer is polysulphone, polyether sulphone or polyetherimide, whereas the hydrophilic polymer is polyvinyl pyrrolidone.
Claim 5
Dependent Claim
46 words
The process according to claims 1-3, characterized in that after applying said small amount of body fluid onto the membrane or composite of membranes that part of the collector membrane whereon/wherein the remaining particulate materials are present and whereon/wherein the non-absorbed fluid is present, is separated.
Claim 6
Dependent Claim
87 words
The process according to claims 1-3, characterized in that said small amount of body fluid respectively whole blood is applied to a first surface of another hydrophilic, microporous membrane (separator membrane), which with its opposite surface is in breakable contact with the collector membrane, one allows the body fluid respectively whole blood to penetrate into the collector membrane until an accurately determined part thereof is saturated, whereupon the separator membrane together with the non-absorbed fluid respectively whole blood and the remained particulate materials present thereon is removed.
Claim 7
Dependent Claim
17 words
The process according to claims 1-5, characterized in that at least asymmetric hydrophilic microporous membrane is used.
Claim 8
Dependent Claim
31 words
The process according to claim 6, characterized in that said small amount of body fluid respectively whole blood is applied to that side of the asymmetric membrane having the largest pores.
Claim 9
Dependent Claim
31 words
The process according to claim 5-7, characterized in that an asymmetric separator membrane is used, which is in contact with the collector membrane with its surface having the smallest pore sizes.
Claim 10
Dependent Claim
31 words
The process according to claim 8, characterized in that an asymmetric collector membrane is used which is in contact with the separator membrane with its surface having the smallest pore size.
Claim 11
Dependent Claim
34 words
The process according to claims 1-9, characterized in that the component(s) in the absorbed body fluid respectively plasma to be determined is (are) reacted with the reagent(s) applied in or on said collector membrane.
Claim 12
Dependent Claim
30 words
The process according to claim 10, characterized in that the physical characteristics which have been changed due to the reaction are determined in a way which is known in itself.
Claim 13
Dependent Claim
70 words
The process according to claims 1-10, characterized in that said collector membrane or an accuretely determined separatable part thereof, together with the therein absorbed body fluid respectively plasma is transferred into a suitable reagent solution, the fluid respectively plasma absorbed into the membrane or part of the membrane is washed out, whereas the physical characteristics changed due to the reaction are determined in a way which is known in itself.
Claim 14
Dependent Claim
30 words
The process for the chemical analysis of components of whole blood according to claims 1-12, characterized in that a hydrophilic microporous membrane is used containing coagulation and/or haemolysis inhibiting substances.
Claim 15
Independent Claim
69 words
A device for the separation of a body fluid from particulate materials in said fluid, in particular plasma from whole blood, to be used for the chemical analysis comprising an inert solid support to which a membrane or composite of membranes is attached, characterized in that said device is provided with one or more hydrophilic microporous membranes from which at least one (collector membrane) has a defined pore volume.
Claim 16
Independent Claim
69 words
A device for the separation of a body fluid from particulate materials in said fluid, in particular plasma from whole blood, to be used for the chemical analysis comprising an inert solid support to which a membrane or composite of membranes is attached, characterized in that said device is provided with one or more hydrophilic microporous membranes from which at least one (collector membrane) has a defined pore volume.
Claim 17
Dependent Claim
35 words
Device according to claim 14, characterized in that each of the hydrophilic microporous membranes essentially consists of a hydrophobic polymer and a hydrophilic polymer, which hydrophilic polymer is fixed in or onto the polymer matrix.
Claim 18
Dependent Claim
24 words
Device according to claim 15, characterized in that said hydrophobic polymer is polysulphone, polyether sulphone or polyetherimide, whereas the hydrophilic polymer is polyvinyl pyrrolidone.
Claim 19
Dependent Claim
31 words
Device according to claims 14-16, characterized in that said device besides said collector membrane also comprises another hydrophilic microporous membrane (separator membrane), which is in breakable contact with said collector membrane.
Claim 20
Dependent Claim
18 words
Device according to claims 14-17, characterized in that said device is provided with an asymmetric hydrophilic microporous membrane.
Claim 21
Dependent Claim
52 words
Device according to claim 18, characterized in that the pore size of the asymmetric membrane on its surface having the largest pores is in the range of 10 µm to 1 mm, whereas the pore size at the surface having the smallest pores is in the range of 0.2 to 5 µm.
Claim 22
Dependent Claim
30 words
Device according to claims 17-19, characterized in that the separator membrane is assymmetric and is in contact with the collector membrane by means of its surface having the smallest pores.
Claim 23
Dependent Claim
29 words
Device according to claim 20, characterized in that said collector membrane is also asymmetric and is in contact with said separator membrane by its surface having the smallest pores.
Claim 24
Dependent Claim
17 words
Device according to claims 14-21, characterized in that said collector membrane has an accurately determined removable part.
Claim 25
Dependent Claim
32 words
Device according to claims 14-22, characterized in that said collector membrane or the accurately determined removable part thereof is attached onto an inert means which is removable from the inert solid support.
Claim 26
Dependent Claim
26 words
Device according to claims 23, characterized in that said removable means, said collector membrane and said separator membrane if any clamps onto the inert solid support.
Claim 27
Dependent Claim
29 words
Device for the separation respectively the chemical analysis of whole blood according to claims 14-25, characterized in that said hydrophilic microporous membrane contains a coagulation or haemolysis inhibiting substance.
Claim 28
Dependent Claim
74 words
Testing kit for the separation of a body fluid from particulate materials in said fluid, in particular plasma from whole blood and for the chemical analysis for the components therein, characterized in that said testing kit is provided with one or more devices according to claims 14-25, one or more solid or liquid reagents contained in one or more suitable containers and optionally one or more solvents contained in one or more separate containers.
Claim 29
Dependent Claim
31 words
Testing kit according to claim 26, further characterized in that it is provided with a suitably packed isotonic solution, which is present in a saturated or unsaturated state in a tissue.
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US3791933A
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EP0175990A2
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