Patent US2595092A successfully refreshed from Google Patents.
EP0333056A2
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Method for inhibiting food-borne human pathogens and preventing microbial spoilage in refrigerated food using a lactobacillus
Assignee: Microlife Technics Inc
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Filing: 1989-03-10
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Granted: —
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Expires: 2009-03-10
Abstract
Abstract A method for inhibiting food-borne pathogen and spoilage microorganisms in processed foods using a Lactobacillus sp. which produces an antimicrobial substance at refrigeration temperatures is described. The method is particularly effective in inhibiting gas producing heterofermentative spoilage microorganisms, mold, food-borne pathogenic microorganisms, ( Listeria and Salmonella ) and psychrotrophic microorganisms which can occur in processed foods.
Inventors
- 👤 Donald P. Boudreaux
- 👤 Mark A. Matrozza
- 👤 Marianne F. Leverone
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 21:37
CPC Classifications
A; A23; A23B; A23B2/00; A23B2/70; A23B2/725; A23B2/729; A23B2/783; A23C; A23C19/00; A23C19/02; A23C19/032; A23C19/0323; A23C9/00; A23C9/12; A23C9/123; A23C9/1234; A23L; A23L13/00; A23L13/70; A23L13/72; A23L13/74; A23V; A23V2400/00; A23V2400/11; A23V2400/125; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/853
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
89 words
-1- A method for inhibition of food-borne human pathogen or spoilage microorganisms in a refrigerated processed food which comprises: (a) inoculating the processed food following any heat treatment of the food which would inactivate the cells with a number of cells of a Lactobacillus which remain viable during refrigerated storage and which produce an antimicrobial substance and to inhibit the microorganisms; and (b) storing the food at refrigeration temperatures which prevent the number of the cells from increasing significantly except during transient temperature abuse to thereby inhibit the microorganisms.
Claim 2
Dependent Claim
25 words
-2- The method of Claim 1 wherein the Lactobacillus is selected from the group consisting of Lactobacillus casei subspecies alactosus NRRL-B-12344 and Lactobacillus sp. NRRL-B-18261.
Claim 3
Dependent Claim
13 words
-3- The method of Claim 1 wherein the Lactobacillus is Lactobacillus sp. NRRL-B-18261.
Claim 4
Dependent Claim
16 words
-4- The method of Claim 1 wherein the refrigeration temperatures are between about 1°C and 12°C.
Claim 5
Dependent Claim
98 words
-5- A method for inhibition of food-borne pathogen or spoilage microorganisms in refrigerated processed foods which comprises: (a) inoculating the processed food following any heat treatment which would inactivate the cells with a number of cells of a Lactobacillus which remains viable during refrigerated storage and which produce an antimicrobial substance; and (b) storing the food at refrigeration temperatures which prevent the number of the added Lactobacillus cells from increasing significantly except during transient temperature abuse, wherein the spoilage microorganisms are inhibited without fermenting the food for at least twice as long as a control without the cells.
Claim 6
Dependent Claim
25 words
-6- The method of Claim 5 wherein the Lactobacillus is selected from the group consisting of Lactobacillus casei subspecies alactosus NRRL-B-12344 and Lactobacillus sp. NRRL-B-18261.
Claim 7
Dependent Claim
13 words
-7- The method of Claim 5 wherein the spoilage microorganisms comprise heterofermentative lactobacilli.
Claim 8
Dependent Claim
24 words
-8- The method of Claim 5 wherein the heterofermentative spoilage microorganisms are selected from the group consisting of Lactobacillus fermentum or Lactobacillus bifermentans .
Claim 9
Dependent Claim
20 words
-9- The method of Claim 5 wherein the food is selected from the group consisting of hard or soft cheeses.
Claim 10
Dependent Claim
13 words
-10- The method of Claim 5 wherein the food is or contains sauce.
Claim 11
Dependent Claim
13 words
-11- The method of Claim 5 wherein the food is a salad dressing.
Claim 12
Dependent Claim
15 words
-12- The method of Claim 5 wherein the food contains milk or a milk derivative.
Claim 13
Dependent Claim
13 words
-13- The method of Claim 5 wherein the food is a prepared salad.
Claim 14
Dependent Claim
13 words
-14- The method of Claim 5 wherein the food is or contains meat.
Claim 15
Dependent Claim
13 words
-15- The method of Claim 5 wherein the food is or contains cheese.
Claim 16
Dependent Claim
14 words
-16- The method of Claim 5 wherein the food is or contains Cottage cheese.
Claim 17
Dependent Claim
20 words
-17- The method of Claim 5 wherein the food is stored at a temperature of between about 1° and 12°C.
Claim 18
Dependent Claim
15 words
-18- The method of Claim 5 wherein the food is provided in a sealed package.
Claim 19
Dependent Claim
26 words
-19- The method of Claim 1 wherein the food is inoculated with between about 10² and 10⁸ cells of the Lactobacillus per gram of the food.
Claim 20
Dependent Claim
19 words
-20- The method of Claim 5 wherein the food is a cheese which can be cracked by gas production.
Claim 21
Dependent Claim
46 words
-21- The method of Claim 1 wherein the Lactobacillus is selected from the group consisting of Lactobacillus casei subspecies alactosus NRRL-B-12344 and Lactobacillus sp. NRRL-B-18261 and wherein the food is inoculated with between about 10² and 10⁸ cells of the Lactobacillus per gram of the food.
Claim 22
Dependent Claim
14 words
-22- The method of Claim 21 wherein the temperature is less than about 12°C.
Claim 23
Dependent Claim
15 words
-23- The method of Claim 17 wherein the temperature is between about 1°C and 12°C.
References Cited (Backward Citations)
No citation records indexed.
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