EP0332416A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Regulation of expression in mammalian cells
Assignee: Dana Farber Cancer Institute Inc
•
Filing: 1989-03-08
•
Granted: —
•
Expires: 2009-03-08
Abstract
Abstract Synthesis of a desired protein in a mammalian cell is regulated by transfecting the cell with a eukaryotic promoter coupled to a DNA sequence encoding a prokaryotic repressor, transfecting the cell with a prokaryotic operator linked to a DNA sequence encoding the desired protein and a promoter driving the transcription of the protein-encoding DNA sequence, the operator and the repressor-encoding DNA sequence being functional in the cell, and culturing the cell in the presence or absence of a substance that binds the repressor.
Inventors
- 👤 David M. Livingston
- 👤 James J. Figge
- 👤 Christopher I. Wright
- 👤 James A. Decaprio
- 👤 Thomas M. Roberts
- 👤 Myles A. Brown
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-10 00:46
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
103 words
A method for achieving expression of a desired protein in a mammalian cell, comprising transfecting said mammalian cell with a eukaryotic promoter coupled to a DNA sequence encoding a prokaryotic repressor, transfecting said mammalian cell with a prokaryotic operator linked to a DNA sequence encoding said desired protein and a promoter driving the transcription of said linked DNA sequence, said operator and said linked DNA sequence being integrated into a chromosome of said cell, said operator and said linked DNA sequence and said promoter and said repressor being functional in said cell, and contacting said cell with a substance that binds said repressor.
Claim 2
Independent Claim
103 words
A method for achieving expression of a desired protein in a mammalian cell, comprising transfecting said mammalian cell with a eukaryotic promoter coupled to a DNA sequence encoding a prokaryotic repressor, transfecting said mammalian cell with a prokaryotic operator linked to a DNA sequence encoding said desired protein and a promoter driving the transcription of said linked DNA sequence, said operator and said linked DNA sequence being integrated into a chromosome of said cell, said operator and said linked DNA sequence and said promoter and said repressor being functional in said cell, and contacting said cell with a substance that binds said repressor.
Claim 3
Dependent Claim
35 words
The method claimed in claim 1 in which said repressor and said operator are both lac or both gal , or wherein said repressor is a trp aporepressor and said operator is a trp operator.
Claim 4
Dependent Claim
20 words
The method claimed in claim 2 in which said substance binding said lac repressor is allolactose or an analog thereof.
Claim 5
Dependent Claim
12 words
The method claimed in claim 3 in which said analog is isopropyl-β-D-thiogalactoside.
Claim 6
Dependent Claim
20 words
The method claimed in claim 2 in which said substance binding said gal repressor is galactose or an analog thereof.
Claim 7
Dependent Claim
20 words
The method claimed in claim 2 in which said substance binding said trp aporepressor is tryptophane or an analog thereof.
Claim 8
Dependent Claim
27 words
A mammalian cell comprising a DNA sequence encoding a functional prokaryotic repressor and a functional cognate operator, said operator being integrated into a chromosome of said cell.
Claim 9
Dependent Claim
27 words
A mammalian cell comprising a DNA sequence encoding a functional prokaryotic repressor and a functional cognate operator, said operator being integrated into a chromosome of said cell.
Claim 10
Dependent Claim
29 words
The cell of claim 7, further comprising, linked to said operator, a DNA sequence encoding a desired protein and a promoter driving the transcription of said linked DNA sequence.
Claim 11
Dependent Claim
13 words
A non-human mammalian organism comprising a cell claimed in claim 7 or 8.
Claim 12
Independent Claim
92 words
A method for regulating production of a desired protein in a non-human mammal which comprises providing said mammal with cells having integrated into a chromosome thereof a functional DNA sequence encoding a prokaryotic operator linked to a DNA sequence encoding said desired protein and a promoter driving the transcription of said linked DNA sequence, said cell also having a functional DNA sequence encoding a prokaryotic repressor coupled to a eukaryotic promoter, and allowing said cells to grow either in the presence or in the absence of a substance binding to said repressor.
Claim 13
Independent Claim
92 words
A method for regulating production of a desired protein in a non-human mammal which comprises providing said mammal with cells having integrated into a chromosome thereof a functional DNA sequence encoding a prokaryotic operator linked to a DNA sequence encoding said desired protein and a promoter driving the transcription of said linked DNA sequence, said cell also having a functional DNA sequence encoding a prokaryotic repressor coupled to a eukaryotic promoter, and allowing said cells to grow either in the presence or in the absence of a substance binding to said repressor.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.