EP0327522A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Production of fused proteins or polypeptides
Assignee: Individual
•
Filing: 1989-02-02
•
Granted: —
•
Expires: 2009-02-02
Abstract
Abstract A method of producing a fusion protein or polypeptide capable of selective bindning to serum albumin, characterized by the steps: a) constructing a recombinant vector comprising a first DNA sequence coding for a serum albumin binding polypeptide fragment and operatively linked to a second DNA sequence coding for a desired protein or polypeptide; b) transforming a compatible host with said recombinant vector such that the combined DNA sequence coding for said fusion protein or polypeptide can be expressed by the host, and culturing the transformed host in a suitable growth medium to produce said fusion protein or polypeptide; and c) isolating said fusion protein or polypeptide; a recombinant vector capable of replication in a microorganism; a host organism transformed by such recombinant vector; and fusion proteins or polypeptides produced according to such method.
Inventors
- 👤 Per-Ake Nygren
- 👤 Lars Abrahmsen
- 👤 Mathias Uhlen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 03:49
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/44; C07K14/445; C07K14/195; C07K14/315; C07K14/76; C07K14/765; C12; C12N; C12N11/00; C12N11/02; C07K2319/00; C07K2319/01; C07K2319/034; C07K2319/70; C07K2319/705
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
106 words
A method of producing a fusion protein or polypeptide capable of selective bindning to serum albumin, characterized by the steps: a) constructing a recombinant vector comprising a first DNA sequence coding for a serum albumin binding polypeptide fragment and operatively linked to a second DNA sequence coding for a desired protein or polypeptide; b) transforming a compatible host with said recombinant vector such that the combined DNA sequence coding for said fusion protein or polypeptide can be expressed by the host, and culturing the transformed host in a suitable growth medium to produce said fusion protein or polypeptide; and c) isolating said fusion protein or polypeptide.
Claim 2
Independent Claim
106 words
A method of producing a fusion protein or polypeptide capable of selective bindning to serum albumin, characterized by the steps: a) constructing a recombinant vector comprising a first DNA sequence coding for a serum albumin binding polypeptide fragment and operatively linked to a second DNA sequence coding for a desired protein or polypeptide; b) transforming a compatible host with said recombinant vector such that the combined DNA sequence coding for said fusion protein or polypeptide can be expressed by the host, and culturing the transformed host in a suitable growth medium to produce said fusion protein or polypeptide; and c) isolating said fusion protein or polypeptide.
Claim 3
Dependent Claim
23 words
A method according to claim 1, wherein the isolation under step c) is performed by making use of the serum albumin bidning capacity.
Claim 4
Dependent Claim
37 words
A method according to claim 2, wherein the isolation of said protein or polypeptide is performed by adsorption to a carrier material containing serum albumin and optionally desorption of the protein or polypeptide from said carrier material.
Claim 5
Dependent Claim
28 words
A method according to claim 1, 2 or 3, wherein said first DNA sequence is constituted by a DNA sequence originating from the gene coding for protein G.
Claim 6
Dependent Claim
29 words
A method according to claim 4, wherein said first DNA sequence is comprised by at least one of segments A1, B1, A2, B2, A3 as shown in Fig. 1.
Claim 7
Dependent Claim
21 words
A method according to claim 5, wherein said first DNA sequence comprises segments B2 and A3 as shown in Fig. 1.
Claim 8
Independent Claim
32 words
A method according to any of claims 3-6, wherein the desorption of the fusion protein or polypeptide from the carrier material is effected by lowering the pH, such as below about 3.
Claim 9
Dependent Claim
44 words
A recombinant vector capable of replication in a microorganism, characterized by comprising a recombinant vec tor containing a first DNA sequence coding for a serum albumin binding polypeptide fragment and operatively linked to a second DNA sequence coding for a desired protein or polypeptide.
Claim 10
Dependent Claim
44 words
A recombinant vector capable of replication in a microorganism, characterized by comprising a recombinant vec tor containing a first DNA sequence coding for a serum albumin binding polypeptide fragment and operatively linked to a second DNA sequence coding for a desired protein or polypeptide.
Claim 11
Dependent Claim
51 words
A recombinant vector according to claim 8, characterized in that one of said DNA sequences extends from the 5'-end of the combined DNA sequence coding for said fusion protein or polypeptide, said fusion protein coding combined sequence preferably comprising the promotor and signal sequence of the structural protein A coding gene.
Claim 12
Dependent Claim
14 words
A host organism transformed by the recombinant vector according to claim 8 or 9.
Claim 13
Dependent Claim
21 words
A host organism according to claim 10, which is a becterial host, such as a strain of Escherichia, Bacillus or Staphylococcus.
Claim 14
Dependent Claim
14 words
A fusion protein or polypeptide produced according to any of claims 1 to 7.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.