EP0318286A2
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EP
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A substratum for cell culture and its production and use
Assignee: Koken Co Ltd
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Filing: 1988-11-24
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Granted: —
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Expires: 2008-11-24
Abstract
Abstract A novel substratum for cell culture, capable of satisfactorily effecting an initial adhesion and proliferation of cells, is provided which comprises a chitosan-collagen composite material as an effective component.
Inventors
- 👤 Teruo Miyata
- 👤 Taira Taira
- 👤 Takashi Kimura
- 👤 Masato Izume
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 17:11
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C08; C08B; C08B37/00; C08B37/0006; C08B37/0024; C08B37/0027; C08B37/003; C08L; C08L5/00; C08L5/08; C12N2533/00; C12N2533/50; C12N2533/54; C12N2533/70; C12N2533/72
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
14 words
A substratum for cell culture, comprising a chitosan-collagen composite material as an effective component.
Claim 2
Dependent Claim
14 words
A substratum for cell culture, comprising a chitosan-collagen composite material as an effective component.
Claim 3
Dependent Claim
50 words
A substratum for cell culture, as claimed in Claim 1, wherein the chitosan-collagen composite material is either a uniform mixture of chitosan and collagen, or comprises a chitosan substrate coated with a thin film layer of collagen, or comprises a collagen substrate coated with a thin film layer of chitosan.
Claim 4
Dependent Claim
46 words
A substratum for cell culture, as claimed in Claim 1 or Claim 2, wherein the chitosan-collagen composite material is crosslinked with a crosslinking agent to increase the mechanical strength, the crosslinking agent optionally being selected from diisocyanates, epihalohydrins, glutaraldehyde, water-soluble carbodiimide, dicyclohexylcarbodiimide, Woodward reagent and DEDQ.
Claim 5
Dependent Claim
49 words
A substratum for cell culture, as claimed in any one of Claims 1 to 3, wherein the chitosan in the chitosan-collagen composite material is selected from chitosan, carboxymethylchitosan, glycol chitosan and succinylchitosan, and/or the collagen in the chitosan-collagen composite material is selected from succinylcollagen, methylated collagens and enzyme-solubilized collagens.
Claim 6
Dependent Claim
44 words
A substratum for cell culture, as claimed in any one of Claims 1 to 4, wherein the ratio of the chitosan and collagen in the chitosan-collagen composite material is 5 to 90% by weight of collagen and 95 to 10% by weight of chitosan.
Claim 7
Dependent Claim
34 words
A substratum for cell structure, as claimed in any one of Claims 1 to 5, wherein the chitosan-collagen composite material is shaped in the form of beads, yarns, hollow fibers, nonwoven cloths or films.
Claim 8
Dependent Claim
146 words
A process for producing a substratum for cell culture, comprising either: (a) adding a base or salt to an acidic solution of chitosan in the presence of collagen to precipitate the chitosan and thus form a chitosan-collagen composite material; or (b) adding a base or salt to an acidic solution of collagen in the presence of chitosan to precipitate the collagen and thus form a chitosan-collagen composite material; or (c) mixing an acidic solution of chitosan and an acidic solution of collagen and then evaporating the solvent from the mixed acidic solution to form a chitosan-collagen composite material; or (d) mixing an acidic solution of chitosan and collagen with a base or salt to precipitate the chitosan and collagen in the solution phase and thus form a chitosan-collagen composite material; in any case the resulting chitosan-collagen composite material optionally being further subjected to a crosslinking treatment.
Claim 9
Dependent Claim
146 words
A process for producing a substratum for cell culture, comprising either: (a) adding a base or salt to an acidic solution of chitosan in the presence of collagen to precipitate the chitosan and thus form a chitosan-collagen composite material; or (b) adding a base or salt to an acidic solution of collagen in the presence of chitosan to precipitate the collagen and thus form a chitosan-collagen composite material; or (c) mixing an acidic solution of chitosan and an acidic solution of collagen and then evaporating the solvent from the mixed acidic solution to form a chitosan-collagen composite material; or (d) mixing an acidic solution of chitosan and collagen with a base or salt to precipitate the chitosan and collagen in the solution phase and thus form a chitosan-collagen composite material; in any case the resulting chitosan-collagen composite material optionally being further subjected to a crosslinking treatment.
Claim 10
Dependent Claim
27 words
A process for producing a substratum for cell culture, as claimed in Claim 7, wherein (a) the collagen is shaped, or wherein (b) the chitosan is shaped.
Claim 11
Dependent Claim
43 words
A process for producing a substratum for cell culture, as claimed in any one of Claims 7 to 9, wherein the chitosan is selected from chitosan, carboxymethylchitosan, glycol chitosan and succinylchitosan, and/or the collagen is selected from succinylcollagen, methylated collagens and enzyme-solubilized collagens.
Claim 12
Dependent Claim
18 words
The use of a substratum as defined in any one of Claims 1 to 6 in cell structure.
References Cited (Backward Citations)
No citation records indexed.
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