EP0317279A2
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EP
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Peptides for use in the purification of factor VIII
Assignee: Scripps Research Institute
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Filing: 1988-11-16
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Granted: —
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Expires: 2008-11-16
Abstract
Abstract Peptides based on the amino acid sequence of Factor VIII which can be used for the purification of Factor VIII and methods for the preparation of these peptides are disclosed. Methods for the purification of Factor VIII using these peptides are also disclosed.
Inventors
- 👤 Theodore S. Zimmerman
- 👤 Paul A. Foster
- 👤 Richard A. Houghten
- 👤 Carol A. Fulcher
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 11:14
CPC Classifications
C; C07; C07K; C07K7/00; C07K7/04; C07K7/08; C07K16/00; C07K16/18; C07K16/36; C07K14/00; C07K14/435; C07K14/745; C07K14/755
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
29 words
A peptide having an amino acid sequence corresponding to a portion of the sequence of Factor VIII, wherein said peptide can be used for the purification of Factor VIII.
Claim 2
Dependent Claim
29 words
A peptide having an amino acid sequence corresponding to a portion of the sequence of Factor VIII, wherein said peptide can be used for the purification of Factor VIII.
Claim 3
Dependent Claim
22 words
A peptide according to Claim 1 which can be used to raise monoclonal or other antibodies for the purification of Factor VIII.
Claim 4
Dependent Claim
25 words
A peptide according to Claim 1 which can be used to elute Factor VIII from monoclonal or other antibodies for the purification of Factor VIII.
Claim 5
Dependent Claim
35 words
A peptide according to Claims 1, 2 or 3 wherein said peptide has the following amino acid sequence: TDSEMDVVRFDDDNS which amino acid sequence is that of amino acid residues 351-365 of the Factor VIII sequence.
Claim 6
Dependent Claim
35 words
A peptide according to Claims 1, 2 or 3 wherein said peptide has the following amino acid sequence: EEIDYDDTISVEMKK which amino acid sequence is that of amino acid residues 1660-1674 of the Factor VIII sequence.
Claim 7
Dependent Claim
22 words
A peptide comprising a sequential subset of at least three amino acid residues of a peptide according to any of Claims 1-5.
Claim 8
Independent Claim
17 words
A method of preparing the peptide according to Claims 1-6 by recombinant DNA or synthetic peptide techniques.
Claim 9
Independent Claim
52 words
A method of preparing Factor VIII comprising: (a) absorbing Factor VIII from a recombinant produced, plasma or commercial concentrate source onto particles bound to a monoclonal antibody raised against a peptide as claimed in any of claims 1-6; (b) eluting the absorbed Factor VIII; and (c) recovering the highly purified Factor VIII.
Claim 10
Dependent Claim
51 words
The method according to Claim 8 wherein said monoclonal antibody is raised against a peptide of the following amino acid sequence: TDSEMDVVRFDDDNS, which amino acid sequence is that of amino acid residues 351-365 of the Factor VIII sequence, or any sequential subset of at least three amino acids of said peptide.
Claim 11
Dependent Claim
51 words
The method according to Claim 8 wherein said monoclonal antibody is raised against a peptide of the following amino acid sequence: EEIDYDDTISVEMKK, which amino acid sequence is that of amino acid residues 1660-1674 of the Factor VIII sequence, or any sequential subset of at least three amino acids of said peptide.
Claim 12
Independent Claim
54 words
A method of preparing Factor VIII comprising: (a) absorbing Factor VIII from a recombinant-produced, plasma or commercial concentrate source onto particles bound to a monoclonal antibody which binds Factor VIII; (b) eluting the absorbed Factor VIII with a peptide as claimed in any of claims 1-6; and (c) recovering the highly purified Factor VIII.
Claim 13
Independent Claim
54 words
The method according to Claims 8 or 11 wherein the eluant used in step (b) is a peptide of the following amino acid sequence: TDSEMDVVRFDDDNS, which amino acid sequence is that of amino acid residues 351-365 of the Factor VIII sequence, or any sequential subset of at least three amino acids of said peptide.
Claim 14
Independent Claim
54 words
The method according to Claims 8 or 11 wherein the eluant used in step (b) is a peptide of the following amino acid sequence: EEIDYDDTISVEMKK, which amino acid sequence is that of amino acid residues 1660-1674 of the Factor VIII sequence, or any sequential subset of at least three amino acids of said peptide.
Claim 15
Dependent Claim
23 words
The method according to Claim 8 wherein the eluant used in step (b) is a peptide as claimed in any of claims 1-6.
Claim 16
Dependent Claim
17 words
A method according to Claim 8 wherein the eluant used in step (b) is a saline solution.
Claim 17
Dependent Claim
13 words
The method according to Claim 15 wherein the saline solution is calcium chloride.
Claim 18
Dependent Claim
25 words
The method according to Claim 16 wherein the concentration of the calcium chloride solution used in step (b) ranges from about 0.25M to about 0.5M.
Claim 19
Independent Claim
17 words
The method according to Claims 8 or 11 wherein said absorbent particles in step (a) are agarose.
References Cited (Backward Citations)
No citation records indexed.
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