EP0314045A2
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Wavelength dispersion electrophoresis apparatus
Assignee: Hitachi Ltd
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Filing: 1988-10-24
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Granted: —
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Expires: 2008-10-24
Abstract
Abstract In a wavelength dispersion electrophoresis apparatus which detects fluorescences of unequal wavelengths emitted from the samples of DNA, RNA or the like labeled with a plurality of fluorophores, a direct-vision prism (14) is interposed between a two-dimensional fluorescence detector (7) and an electrophoretic plate (17) in order to separate and discriminate the emission wavelengths of the respective fluorophores. The direct-vision prism (14) is disposed for the wavelength dispersion of the fluorescences in this manner, whereby the fluorescences of the individual wavelengths can be separated and detected at a high sensitivity by a simple mechanism and without distorting a fluorescence image.
Inventors
- 👤 Hideki Kambara
- 👤 Yoshitoshi Ito
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 03:20
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721; G01N2021/6417; G01N2021/6421; G01N2021/6439; G01N21/645; G01N21/6456
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
92 words
A wavelength dispersion electrophoresis apparatus having means (31) for electrophoretically separating a sample group which consists of samples labeled with at least two kinds of fluorophores, excitation means (13) for exciting the fluorophore which has labeled the sample, thereby causing it to emit fluorescence, and detection means (7) for detecting the emitted fluorescence; characterized in that a composite prism (14) in which a plurality of unit prisms (20...22) made of different materials are combined and which disperses the fluorescences at respective fluorescence wavelengths is disposed in front of said detection means (7).
Claim 2
Dependent Claim
92 words
A wavelength dispersion electrophoresis apparatus having means (31) for electrophoretically separating a sample group which consists of samples labeled with at least two kinds of fluorophores, excitation means (13) for exciting the fluorophore which has labeled the sample, thereby causing it to emit fluorescence, and detection means (7) for detecting the emitted fluorescence; characterized in that a composite prism (14) in which a plurality of unit prisms (20...22) made of different materials are combined and which disperses the fluorescences at respective fluorescence wavelengths is disposed in front of said detection means (7).
Claim 3
Dependent Claim
81 words
The apparatus of claim 1, wherein the electrophoresis separation means is configured of a plurality of migration lanes (31) which are set in parallel, said excitation means includes light (13) which intersects all of said migration lanes (31) and which enters perpendicularly to said migration lanes (31), said detection means includes a two-dimensional detector (7), and said composite prism (14) and said detection means (7) are so arranged that the wavelength dispersion occurs in a direction perpendicular to the fluorescence image.
Claim 4
Dependent Claim
24 words
The apparatus of claim 2, wherein a filter (4) for cutting off the excitation light is disposed in front of said composite prism (14).
Claim 5
Dependent Claim
116 words
A wavelength dispersion electrophoresis apparatus comprising i) an electrophoretic plate (17) which includes a plurality of migration lanes (31) for electrophoresis of a sample group consisting of samples labeled with at least two kinds of fluorophores, ii) a filter (4) which cuts off excitation light, iii) a composite prism (14) in which a plurality of unit prisms (20...22) made of different materials are combined, iv) a condensing lens (5), v) an image intensifier (6), vi) a two-dimensional fluorescence detector (7), the constituents i) thru vi) being disposed in this order, and means for causing a laser beam (13) as said excitation light to enter so as to intersect all of said migration lanes (31) substantially orthogonally.
Claim 6
Dependent Claim
116 words
A wavelength dispersion electrophoresis apparatus comprising i) an electrophoretic plate (17) which includes a plurality of migration lanes (31) for electrophoresis of a sample group consisting of samples labeled with at least two kinds of fluorophores, ii) a filter (4) which cuts off excitation light, iii) a composite prism (14) in which a plurality of unit prisms (20...22) made of different materials are combined, iv) a condensing lens (5), v) an image intensifier (6), vi) a two-dimensional fluorescence detector (7), the constituents i) thru vi) being disposed in this order, and means for causing a laser beam (13) as said excitation light to enter so as to intersect all of said migration lanes (31) substantially orthogonally.
Claim 7
Dependent Claim
35 words
The apparatus of claim 4, wherein said laser beam (13) is a collimated beam having a circular cross-section and a diameter of at most 0.3 mm in the measurement region of said electrophoretic plate (17).
References Cited (Backward Citations)
No citation records indexed.
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