EP0300082A2
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Enzyme electrode
Assignee: Bridgestone Corp
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Filing: 1987-09-03
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Granted: —
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Expires: 2007-09-03
Abstract
Abstract An enzyme electrode comprises an enzyme immobilized on a polyaniline or a derivative thereof prepared by electrolytic oxidative polymerization or chemical oxydative polymerization using an aqueous acidic solution containing aniline or a derivative thereof. The enzyme electrode is excellent in the current dependency on oxygen concentration and the mediator leaching property and suitable for use in fuel cells, bioreactors, biosensors, etc.
Inventors
- 👤 Tadashi Nakajima
- 👤 Yasuhiro Iino
- 👤 Takahiro Kawagoe
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 03:33
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
36 words
An enzyme electrode comprising immobilizing an enzyme on a polyaniline or a derivative thereof prepared by an electrolytic oxidative polymerization or a chemical oxydative polymerization using an aqueous acidic solution containing aniline or a derivative thereof.
Claim 2
Dependent Claim
36 words
An enzyme electrode comprising immobilizing an enzyme on a polyaniline or a derivative thereof prepared by an electrolytic oxidative polymerization or a chemical oxydative polymerization using an aqueous acidic solution containing aniline or a derivative thereof.
Claim 3
Dependent Claim
67 words
The enzyme electrode according to claim 1, wherein the electrolytic oxidative polymerization is carried out by using an aqueous acidic solution comprising aniline or a derivative thereof in an aniline amount of from 0.01 to 5 mol/l and an acid in an amount of from 0.02 to 10 mol/l at a current density of 10 µA/cm² to 20 mA/cm² and at a temperature of 0 to 40°C.
Claim 4
Dependent Claim
34 words
The enzyme electrode according to claim 2, wherein the acid is selected from the group consisting of hydrofluoric acid, hydrochloric acid, nitric acid, perchloric acid, borofluoric acid, sulfuric acid, acetic acid and phosphoric acid.
Claim 5
Dependent Claim
68 words
The enzyme electrode according to claim 1, wherein the chemical oxydative polymerization is carried out by using an aqueous acidic solution comprising aniline or a derivative thereof in an aniline amount of from 0.01 to 5 mol/l, an acid in an amount of from 0.02 to 10 mol/l and an oxidizing agent in an amount of from 0.01 to 5 mol/l at a temperature of 0 to 40°C.
Claim 6
Dependent Claim
34 words
The enzyme electrode according to claim 4, wherein the acid is selected from the group consisting of hydrofluoric acid, hydrochloric acid, nitric acid, perchloric acid, borofluoric acid, sulfuric acid, acetic acid and phosphoric acid.
Claim 7
Dependent Claim
27 words
The enzyme electrode according to claim 4, wherein the oxidizing agent is selected from the group consisting of ferric chloride, ammonium persulfate, potassium bichromate and potassium permanganate.
Claim 8
Dependent Claim
68 words
The enzyme electrode according to claim 1, wherein a reduction treatment is electrically carried out to the polyaniline or the derivative thereof at a silver/silver chloride electrode potential of from -0.6 V to +0.2 V and at a current density of from 1 to 5 mA/cm² by immersing the polyaniline or the derivative thereof as a cathode in an electrolytic solution having a pH of lower than 8.
Claim 9
Dependent Claim
52 words
The enzyme electrode according to claim 1, wherein a cyclic voltammetry is applied to the polyaniline or the derivative thereof at 5 to 50 mV/sec within a range from -0.2 to +0.7 V by immersing the polyaniline or the derivative thereof in an electrolytic solution having a pH of lower than 8.
Claim 10
Dependent Claim
38 words
The enzyme electrode according to claim 1, wherein a reduction treatment is chemically carried out to the polyaniline or the derivative thereof by immersing the polyaniline or the derivative thereof in an aqueous solution containing a reducing agent.
Claim 11
Dependent Claim
35 words
The enzyme electrode according to claim 1, wherein a neutralization treatment is applied to the polyaniline or the derivative thereof by immersing in or washing by a solution having a pH of 3 to 10.
Claim 12
Dependent Claim
38 words
The enzyme electrode according to claim 1, wherein the enzyme is selected from the group consisting of glucose oxidase, alcohol dehydrogenase, urease, glucokinase, peroxidase, cholesterol esterase, lipase, phospholipase, catalase, lactic acid dehydrogenase, glucoamylase, galactose oxidase, pennicillinase and tyronase.
References Cited (Backward Citations)
No citation records indexed.
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