EP0294996A2
Expired / Lapsed
EP
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Scanning fluorescent detection system
Assignee: EIDP Inc
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Filing: 1988-06-01
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Granted: —
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Expires: 2008-06-01
Abstract
Abstract A system for detecting the radiant energy emitted from different closely spaced species includes two detectors each having a large entrance angle for receiving the radiant energy, and wavelength selective filters between the detector and species, the transmission vs. wavelength characteristics being complementary, and means for ratioing functions of the detector outputs, the ratio being indicative of the identity of the species.
Inventors
- 👤 Charles William Robertson, Jr.
- 👤 Rudy Johan Dam
- 👤 James Merrill Prober
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 20:30
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/645; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721; G01J; G01J1/00; G01J1/02; G01J1/04; G01J1/06; G01J2001/062; G01J3/00; G01J3/12; G01J2003/1226; G01N2021/6417; G01N2021/6421; G01N21/6428; G01N2021/6439; G01N2021/6441
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
234 words
A system for detecting the presence of radiant energy emitted from different species, following separation in time and/or space, and the identity of such species, having first detection means responsive to the radiant energy emitted by the species for generating a first signal that varies in amplitude in a first sense as a function of the nature of the species, second detection means responsive to the radiant energy for generating a second signal that varies in amplitude in a second sense different than the first sense as a function of the nature of the species, and third means responsive to the first and second signals for obtaining a third signal corresponding to the ratio of functions of the first and second signals, the amplitude of the third signal being indicative of the identity of each of the species, characterised in that the first and second detection means each include: a detector having a large solid entrance angle positioned adjacent the species to receive radiant energy emitted from the species for generating one of the first and second signals, and a wavelength selective filter positioned between each respective detector and the species, each wavelength filter having transmission vs. wavelength characteristics that are complementary; and in that one of the first and second detection means includes a transmission filter for rejecting radiant energy incident on the transmission filter at an angle greater than a predetermined value.
Claim 2
Independent Claim
234 words
A system for detecting the presence of radiant energy emitted from different species, following separation in time and/or space, and the identity of such species, having first detection means responsive to the radiant energy emitted by the species for generating a first signal that varies in amplitude in a first sense as a function of the nature of the species, second detection means responsive to the radiant energy for generating a second signal that varies in amplitude in a second sense different than the first sense as a function of the nature of the species, and third means responsive to the first and second signals for obtaining a third signal corresponding to the ratio of functions of the first and second signals, the amplitude of the third signal being indicative of the identity of each of the species, characterised in that the first and second detection means each include: a detector having a large solid entrance angle positioned adjacent the species to receive radiant energy emitted from the species for generating one of the first and second signals, and a wavelength selective filter positioned between each respective detector and the species, each wavelength filter having transmission vs. wavelength characteristics that are complementary; and in that one of the first and second detection means includes a transmission filter for rejecting radiant energy incident on the transmission filter at an angle greater than a predetermined value.
Claim 3
Dependent Claim
42 words
A system as set forth in claim 1 which also includes a laser adapted to direct an exciting beam of radiant energy to the species, the laser beam of radiant energy having a wavelength lying within the excitation region of the species.
Claim 4
Dependent Claim
32 words
A system as set forth in claim 2 wherein the transmission filter is associated with the wavelength selective filter having a passband closest to the wavelength of the laser beam radiant energy.
Claim 5
Dependent Claim
36 words
A system as set forth in claim 1, 2 or 3 which includes means adapted to separate fragments of DNA or other molecules labelled with emitting species of materials, positioned to be excited by radiant energy.
Claim 6
Dependent Claim
45 words
A system as set forth in claim 4 when appendant to claim 2, in which the radiant energy to be detected by the means adapted to separate fragments of DNA or other molecules labelled with emitting species of materials, is radiant energy from the laser.
Claim 7
Independent Claim
31 words
A system, as set forth in any one of claims 1 to 5, wherein the first and second detection means are positioned on opposite sides of the region containing exciting radiation.
Claim 8
Dependent Claim
36 words
A system as set forth in claim 6, in combination with claim 2 or any one of claims 3 to 5 when appendant thereto, wherein the region is that propaagating the laser beam of radiant energy.
Claim 9
Independent Claim
34 words
A system as set forth in any one of claims 1 to 7 which includes means to sweep the laser beam of radiant energy across the separation means to excite the species in sequence.
Claim 10
Dependent Claim
175 words
Apparatus for detecting the presence of fluorescent radiation derived from fragments of DNA or other molecules labelled according to type with different fluorescing species of materials comprising: separation means adapted to spacially separate the molecules, means including a laser to sweep in a first plane a beam of radiant energy across the separation means to excite the species, first and second photodetectors having large solid entrance angle positioned on opposite sides of the first plane to convert fluorescent radiation emitted from the species into first and second signals, first and second wavelength filters having complementary transmission - wavelength characteristics interposed between the respective photodetectors and the separation means, a transmission filter, interposed between one of the respective photodetectors and its separation means, for rejecting radiation incident on the photodetector at an angle greater than a predetermined value, and means responsive to the first and second signals for deriving a third signal corresponding to the ratio of functions of the first and second signals, the third signal being indicative of the identity of the fluorescing species.
Claim 11
Dependent Claim
175 words
Apparatus for detecting the presence of fluorescent radiation derived from fragments of DNA or other molecules labelled according to type with different fluorescing species of materials comprising: separation means adapted to spacially separate the molecules, means including a laser to sweep in a first plane a beam of radiant energy across the separation means to excite the species, first and second photodetectors having large solid entrance angle positioned on opposite sides of the first plane to convert fluorescent radiation emitted from the species into first and second signals, first and second wavelength filters having complementary transmission - wavelength characteristics interposed between the respective photodetectors and the separation means, a transmission filter, interposed between one of the respective photodetectors and its separation means, for rejecting radiation incident on the photodetector at an angle greater than a predetermined value, and means responsive to the first and second signals for deriving a third signal corresponding to the ratio of functions of the first and second signals, the third signal being indicative of the identity of the fluorescing species.
Claim 12
Dependent Claim
47 words
A system as set forth in any one of claims 1 to 8 or an apparatus as set forth in claim 9, wherein the wavelength selective filters have a transition in their transmission vs. wavelength characteristics centered at about the middle of the species' radiant energy spectra.
Claim 13
Dependent Claim
19 words
Apparatus according to claim 10, wherein the transition is centered at about the middle of the species fluorescent radiation.
Claim 14
Independent Claim
41 words
A system or apparatus as set forth in any one of claims 1 to 11, wherein the transmission filter has an extra mural absorber among plural optical fibres positioned to have parallel generatrices transverse to the first and second detection means.
Claim 15
Dependent Claim
28 words
A system or apparatus as set forth in claim 12 characterized by the absence of a lens between the first and second responsive means and the emitting species.
Claim 16
Dependent Claim
27 words
A system or apparatus as set forth in claim 12 or 13, wherein the predetermined value of the rejecting angle of the transmission filter is about 22°.
Claim 17
Independent Claim
24 words
A system as set forth in any one of claims 1 to 14, wherein both of said detection means have a said transmission filter.
References Cited (Backward Citations)
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