EP0292802A2
Expired / Lapsed
EP
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Process for the preparation of double stranded DNA
Assignee: Hoechst AG
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Filing: 1988-05-13
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Granted: —
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Expires: 2008-05-13
Abstract
Abstract It is possible by synthesis of a DNA single strand with a hairpin structure at one or both ends, filling in to give the double strand and opening or elimination of the hairpin structure(s) to obtain even very long double-stranded DNA fragments or genes. It is also possible to attach mutagenic primers to the single strand and thus to carry out site-specific mutagenesis. Furthermore, the preparation of highly labelled DNA sequences is possible in the filling-in reaction. <IMAGE>
Inventors
- 👤 Eugen Dr. Uhlmann
- 👤 Friedrich Dr. Hein
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 19:13
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6844; C12Q1/6853; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/585; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1096; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34
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