Patent US2589711A successfully refreshed from Google Patents.
EP0289034A2
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EP
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Transkaryotic implantation
Assignee: General Hospital Corp
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Filing: 1988-04-29
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Granted: —
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Expires: 2008-04-29
Abstract
Abstract The present invention provides a method of altering the concentration or level of expression of a desired gene product in a recipient subject which involves administering to a recipient a transkaryotic cell capable of expressing a desired gene when the cell is present within the subject. The invention further pertains to compounds produced by such cells, by transkaryotic animals in response to such cells, and to the uses of such compounds.
Inventors
- 👤 Richard F. Selden
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 02:24
CPC Classifications
A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; A61; A61K; A61K38/00; A01K67/0271; A61K38/16; A61K38/17; A61K38/22; A61K38/27; A61K38/28; A61K49/00; A61K49/0004; A61K49/0008; A61K9/00; A61K9/0012; A61K9/0019; A61K9/0024; C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; C07K14/62; C07K16/00; C07K16/18; C07K16/26; A01K2217/00; A01K2217/05; A01K2267/00; A01K2267/03; A01K2267/035; A01K2267/0362; A61K39/00; A61K2039/51; A61K2039/515; A61K2039/5156; A61K48/00; C12; C12N; C12N2510/00; C12N2510/02
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
66 words
A method of altering the concentration of a desired gene product in a recipient subject which comprises providing to said recipient subject a transfected cell preparation, said preparation containing at least one transfected cell which contains a desired gene sequence, wherein said cell, when provided to said subject, will direct the expression of said desired gene sequence thereby causing the production of a desired gene product.
Claim 2
Independent Claim
66 words
A method of altering the concentration of a desired gene product in a recipient subject which comprises providing to said recipient subject a transfected cell preparation, said preparation containing at least one transfected cell which contains a desired gene sequence, wherein said cell, when provided to said subject, will direct the expression of said desired gene sequence thereby causing the production of a desired gene product.
Claim 3
Independent Claim
66 words
A method of altering the concentration of a desired gene product in a recipient subject which comprises providing to said recipient subject a transfected cell preparation, said preparation containing at least one transfected cell which contains an effector gene sequence, wherein said cell, when provided to said subject, will direct the expression of said effector gene sequence thereby causing the production of a desired gene product.
Claim 4
Independent Claim
66 words
A method of altering the concentration of a desired gene product in a recipient subject which comprises providing to said recipient subject a transfected cell preparation, said preparation containing at least one transfected cell which contains an effector gene sequence, wherein said cell, when provided to said subject, will direct the expression of said effector gene sequence thereby causing the production of a desired gene product.
Claim 5
Independent Claim
23 words
The method of any one of claims 1-2 wherein said desired or effector gene sequence is operably linked to a constitutive promoter region.
Claim 6
Independent Claim
23 words
The method of any one of claims 1-2 wherein said desired or effector gene sequence is operably linked to a regulatable promoter region.
Claim 7
Independent Claim
28 words
The method of any one of claims 1-2 wherein said transfected cell was originally obtained from an animal of the same species as that of the subject recipient.
Claim 8
Dependent Claim
16 words
The method of claim 5 wherein said transfected cell was originally obtained from said recipient subject.
Claim 9
Independent Claim
35 words
The method of any one of claims 1-2 wherein the expression of said desired or effector gene sequence provides to said recipient subject a gene product which had not previously been expressed by said subject.
Claim 10
Dependent Claim
23 words
The method of claim 7 wherein said expressed desired or effector gene sequence is equivalent to a native gene of said recipient subject.
Claim 11
Independent Claim
40 words
The method of any one of claims 1-2 wherein the expression of said desired or effector gene sequence in said recipient subject causes an increase in the level of expression of a gene which is normally expressed by said subject.
Claim 12
Dependent Claim
24 words
The method of claim 9 wherein said expression of said desired gene sequence compensates for a deficiency of gene expression in said recipient subject.
Claim 13
Independent Claim
38 words
The method of any one of claims 1-2 wherein the expression of said desired gene sequence in said recipient subject causes a decrease in the level of expression of a gene which is normally expressed by said subject.
Claim 14
Dependent Claim
25 words
The method of claim 11 wherein said expression of said desired gene sequence compensates for an excessive level of gene expression in said subject recipient.
Claim 15
Independent Claim
21 words
The method of any one of claims 1-2 wherein said expression of said desired or effector gene sequence is physiologically significant.
Claim 16
Independent Claim
50 words
The method of any one of claims 1-2 wherein said transfected cell preparation is provided to said recipient subject by means selected from the group consisting of: subcapsular implantation, subdermal implantation, intraperitoneal implantation, intracranial implantation, intrahepatic implantation, retroperitoneal implantation, intramuscular implantation, intrapulmonary implantation, intraocular implantation, intratesticular implantation, or intrasplanchnic implantation.
Claim 17
Dependent Claim
19 words
The method of claim 14 wherein said transfected cell preparation is provided to said recipient subject by subcapsular implantation.
Claim 18
Dependent Claim
19 words
The method of claim 14 wherein said transfected cell preparation is provided to said recipient subject by subdermal implantation.
Claim 19
Independent Claim
85 words
A method for inducing the production of a biological compound which comprises providing to a recipient subject an effective amount of a tranfected cell preparation, said preparation containing at least one transfected cell which contains a desired gene sequence, wherein said cell, when provided to said subject, will direct the expression of said desired gene sequence thereby causing the production of a desired gene product (I); the expression of said desired gene sequence being sufficient to induce a recipient subject to product said biological compound.
Claim 20
Independent Claim
85 words
A method for inducing the production of a biological compound which comprises providing to a recipient subject an effective amount of a tranfected cell preparation, said preparation containing at least one transfected cell which contains a desired gene sequence, wherein said cell, when provided to said subject, will direct the expression of said desired gene sequence thereby causing the production of a desired gene product (I); the expression of said desired gene sequence being sufficient to induce a recipient subject to product said biological compound.
Claim 21
Dependent Claim
18 words
The method of claim 17 wherein said biological molecule is capable of binding to said desired gene product.
Claim 22
Dependent Claim
20 words
The method of claim 18 wherein said desired gene product is an antigen, and said biological compounds is an antibody.
Claim 23
Dependent Claim
33 words
The method of claim 18 wherein said desired gene product is a fragment of a complete gene product and said biological compound is a region-specific antibody with respect to said complete gene product.
Claim 24
Dependent Claim
75 words
A method for determining the concentration of a desired gene product (II) in a sample which comprises: (a) incubating said sample in the presence of a biological compound capable of binding said desired gene product (II), the production of said biological compound being induced by the method of claim 17, (b) determining the concentration of said desired gene product (II) by measuring the amount of said biological compound bound to said desired gene product (II).
Claim 25
Dependent Claim
25 words
The method of claim 21 wherein said gene product (I) and said gene product (II) are identical and wherein said biological compound is an antibody.
Claim 26
Dependent Claim
34 words
The method of claim 21 wherein said gene product (I) is a fragment of said gene product (II) and wherein said biological compound is a region-specific antibody with respect to said gene product (II).
Claim 27
Dependent Claim
79 words
A method for determining the concentration of a desired gene product (II) in a sample which comprises: (a) incubating said sample in the presence of two different biological compounds capable of binding said desired gene product (II), the production of at least one of said biological compounds being induced by the method of claim 17, (b) determining the concentration of said desired gene product (II) by measuring the amount of said biological compounds bound to said desired gene product.
Claim 28
Dependent Claim
29 words
The method of claim 24 wherein said gene product (I) and said gene product (II) are identical and wherein at least one of said biological compounds is an antibody.
Claim 29
Dependent Claim
34 words
The method of claim 25 wherein said gene product (I) is a fragment of said gene product (II) and wherein said biological compound is a region-specific antibody with respect to said gene product (II).
Claim 30
Dependent Claim
9 words
An antibody produced by the method of claim 19.
Claim 31
Dependent Claim
10 words
A region-specific antibody produced by the method of claim 20.
Claim 32
Independent Claim
60 words
A method for producing a monoclonal antibody which comprises: (a) inducing the production of antibody by the method of any one of claims 19-20, (b) removing a cell from said recipient subject, said cell being capable of producing said antibody, (c) forming a hybridoma between said cell (b) and an immortalized myeloma cell, wherein said hybridoma cell produces said antibody.
Claim 33
Dependent Claim
7 words
An implant which comprises a transfected cell.
Claim 34
Dependent Claim
7 words
An implant which comprises a transfected cell.
Claim 35
Dependent Claim
12 words
The implant of claim 30 wherein said implant is a subcapsular implant.
Claim 36
Dependent Claim
12 words
The implant of claim 30 wherein said implant is an intraperitoneal implant.
References Cited (Backward Citations)
No citation records indexed.
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