EP0288687A2
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Monoclonal antibody, hybridoma, their production and use thereof
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1988-03-01
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Granted: —
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Expires: 2008-03-01
Abstract
Abstract A monoclonal antibody is produced from a cloned hybridoma, and the monoclonal antibody combines specifically with basic fibroblast growth factor (bFGF). Therefore, the monoclonal antibody can be advantageously used for assay reagents on bFGF or for purification of bFGF.
Inventors
- 👤 Makoto Iwane
- 👤 Tsutomu Kurokawa
- 👤 Koichi Igarashi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 03:55
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/22; C07K14/00; C07K14/435; C07K14/475; C07K14/50; C07K14/503; C07K14/52; C07K14/54; C07K14/55; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
50 words
A monoclonal antibody which combines specifically with basic fibroblast growth factor (bFGF), the antibody having the characteristics: (a) it has a molecular weight of about 140 to 160 kilodaltons, (b) it does not cross-react with acidic fibroblast growth factor, and (c) it belongs to the immunoglobulin class IgM or IgG.
Claim 2
Dependent Claim
50 words
A monoclonal antibody which combines specifically with basic fibroblast growth factor (bFGF), the antibody having the characteristics: (a) it has a molecular weight of about 140 to 160 kilodaltons, (b) it does not cross-react with acidic fibroblast growth factor, and (c) it belongs to the immunoglobulin class IgM or IgG.
Claim 3
Dependent Claim
21 words
A monoclonal antibody as claimed in Claim 1, wherein the bFGF is a polypeptide which includes the amino acid sequence: Phe-Phe-Leu-Arg-Ile-His-Pro-Asp-Gly-Arg-Val-Asp-Gly-Val-Arg-Glu-Lys-Ser-Asp-Pro.
Claim 4
Dependent Claim
24 words
A cloned hybridoma comprising a splenic cell from a mammal immunized with basic fibroblast growth factor (bFGF) and a homogenic or heterogenic lymphoid cell.
Claim 5
Dependent Claim
24 words
A cloned hybridoma comprising a splenic cell from a mammal immunized with basic fibroblast growth factor (bFGF) and a homogenic or heterogenic lymphoid cell.
Claim 6
Dependent Claim
13 words
A hybridoma as claimed in Claim 3, wherein the mammal is a mouse.
Claim 7
Dependent Claim
15 words
A hybridoma as claimed in Claim 3, wherein the lymphoid cell is a myeloma cell.
Claim 8
Dependent Claim
20 words
A hybridoma as claimed in Claim 3, wherein the bFGF is a polypeptide which includes the amino acid sequence: Phe-Phe-Leu-Arg-Ile-His-Pro-Asp-Gly-Arg-Val-Asp-Gly-Val-Arg-Glu-Lys-Ser-Asp-Pro.
Claim 9
Independent Claim
93 words
A method for producing a monoclonal antibody which combines specifically with basic fibroblast growth factor (bFGF) and has the characteristics: (a) it has molecular weight of about 140 to 160 kilodaltons, (b) it does not cross-react with acidic fibroblast growth factor, and (c) it belongs to the immunoglobulin class IgM or IgG, which comprises growing a cloned hybridoma comprising a splenic cell from a mammal immunized with said bFGF and a homogenic or heterogenic lymphoid cell in liquid medium or mammalian abdomen to allow the hybridoma to produce and accumulate the monoclonal antibody.
Claim 10
Independent Claim
93 words
A method for producing a monoclonal antibody which combines specifically with basic fibroblast growth factor (bFGF) and has the characteristics: (a) it has molecular weight of about 140 to 160 kilodaltons, (b) it does not cross-react with acidic fibroblast growth factor, and (c) it belongs to the immunoglobulin class IgM or IgG, which comprises growing a cloned hybridoma comprising a splenic cell from a mammal immunized with said bFGF and a homogenic or heterogenic lymphoid cell in liquid medium or mammalian abdomen to allow the hybridoma to produce and accumulate the monoclonal antibody.
Claim 11
Dependent Claim
13 words
A method as claimed in Claim 7, wherein the mammal is a mouse.
Claim 12
Dependent Claim
15 words
A method as claimed in Claim 7, wherein the lymphoid cell is a myeloma cell.
Claim 13
Independent Claim
43 words
A method for producing a cloned hybridoma comprising a splenic cell from mammal immunized with basic fibroblast growth factor (bFGF) and a homogenic or heterogenic lymphoid cell, which comprises subjecting said splenic cell and said lymphoid cell to cell fusion followed by cloning.
Claim 14
Independent Claim
43 words
A method for producing a cloned hybridoma comprising a splenic cell from mammal immunized with basic fibroblast growth factor (bFGF) and a homogenic or heterogenic lymphoid cell, which comprises subjecting said splenic cell and said lymphoid cell to cell fusion followed by cloning.
Claim 15
Dependent Claim
13 words
A method as claimed in Claim 10, wherein the mammal is a mouse.
Claim 16
Dependent Claim
15 words
A method as claimed in Claim 10, wherein the lymphoid cell is a myeloma cell.
Claim 17
Dependent Claim
53 words
Use of a monoclonal antibody which combines specifically with basic fibroblast growth factor (bFGF) and has the characteristics: (a) it has molecular weight of about 140 to 160 kilodaltons, (b) it does not cross-react with acidic fibroblast growth factor, and (c) it belongs to the immunoglobulin class IgM or IgG., to purify bFGF.
Claim 18
Dependent Claim
53 words
Use of a monoclonal antibody which combines specifically with basic fibroblast growth factor (bFGF) and has the characteristics: (a) it has molecular weight of about 140 to 160 kilodaltons, (b) it does not cross-react with acidic fibroblast growth factor, and (c) it belongs to the immunoglobulin class IgM or IgG., to purify bFGF.
Claim 19
Dependent Claim
55 words
Use of a monoclonal antibody which combines specifically with basic fibroblast growth factor (bFGF) and has the characteristics: (a) it has molecular weight of about 140 to 160 kilodaltons, (b) it does not cross-react with acidic fibroblast growth factor, and (c) it belongs to the immunoglobulin class IgM or IgG., to detect or measure bFGF.
Claim 20
Dependent Claim
55 words
Use of a monoclonal antibody which combines specifically with basic fibroblast growth factor (bFGF) and has the characteristics: (a) it has molecular weight of about 140 to 160 kilodaltons, (b) it does not cross-react with acidic fibroblast growth factor, and (c) it belongs to the immunoglobulin class IgM or IgG., to detect or measure bFGF.
References Cited (Backward Citations)
No citation records indexed.
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