EP0288425A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
An apparatus for the treatment of biological samples and treatment methods using the same
Assignee: Sekisui Chemical Co Ltd
•
Filing: 1988-04-22
•
Granted: —
•
Expires: 2008-04-22
Abstract
Abstract An apparatus for the treatment of biological samples comprises: at least one column having an adsorbent layer made of adsorbent that fills a part of said column, and a reservoir forming the other part of said column; at least one vessel connected to said reservoir, said vessel being disposed so as to contain said samples to be supplied to said reservoir and/or media for treating said samples; a means for supplying said samples and/or media for treating said samples from said vessel to said reservoir; and a means for controlling said supply means. A method for the treatment of biological samples involves the use of the above-mentioned apparatus. With the use of the apparatus, the analysis of samples and also the isolation of desired substances from samples could be done automatically with accuracy in a simple procedure.
Inventors
- 👤 Sho Kikyotani
- 👤 Tatsuo Yamamoto
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 13:07
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/88; G01N2030/8804; G01N2030/8809; G01N2030/8813; G01N2030/8827; G01N30/04; G01N30/06; G01N30/26; G01N30/38; G01N30/46; G01N30/466
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
93 words
An apparatus for the treatment of biological samples comprising: at least one column with an adsorbent layer made of adsorbent that fills a part of said column, and a reservoir forming the other part of said column; at least one vessel connected to said reservoir, said vessel being disposed so as to contain said samples and/or media for treating said samples to be supplied to said reservoir: a means for supplying said samples and/or media for treating said samples from said vessel to said reservoir; and a means for controlling said supply means.
Claim 2
Independent Claim
93 words
An apparatus for the treatment of biological samples comprising: at least one column with an adsorbent layer made of adsorbent that fills a part of said column, and a reservoir forming the other part of said column; at least one vessel connected to said reservoir, said vessel being disposed so as to contain said samples and/or media for treating said samples to be supplied to said reservoir: a means for supplying said samples and/or media for treating said samples from said vessel to said reservoir; and a means for controlling said supply means.
Claim 3
Dependent Claim
22 words
An apparatus according to claim 1 , wherein said column is held in a column holder that is connected with an agitator.
Claim 4
Dependent Claim
112 words
A method for the treatment of biological samples by the use of the apparatus of claim 1 , comprising the steps of: supplying said samples from said vessel to said reservoir via said supply means or supplying said samples directly to said reservoir; allowing said samples to permeate the adsorbent layer of said column, which causes said adsorbent to adsorb components of said samples; and eluting said components from said adsorbent layer by supplying the media for treating said samples from said vessel to said adsorbent layer via said supply means, said supply of the samples and the media by the use of said supply means being controlled by said controlling means.
Claim 5
Dependent Claim
142 words
A method for the treatment of biological samples by the use of the apparatus of claim 1 , comprising the steps of: supplying said samples from said vessel to said reservoir via said supply means or supplying said samples directly to said reservoir; allowing said samples to permeate the adsorbent layer of said column, which causes said adsorbent to adsorb components of said sample; supplying media for washing from said vessel to said adsorbent layer of the column via said supply means, so that said adsorbent layer is washed with said media for washing; and eluting said components from said adsorbent layer by supplying the media for treating said samples from said vessel to said adsorbent layer via said supply means, said supply of the samples and the media by the use of said supply means being controlled by said controlling means.
Claim 6
Dependent Claim
53 words
A method according to claim 3 or 4 , wherein said samples supplied to said reservoir of the column are pretreated in said reservoir with the use of media for pretreating said samples, said media being supplied from said vessel to said reservoir via said supply means or supplied directly to said reservoir.
Claim 7
Dependent Claim
31 words
A method according to claim 5 , wherein said samples contain cells, and said cells are lysed when pretreated in said reservoir, by the use of media for pretreating said samples.
Claim 8
Dependent Claim
17 words
A method according to claim 5 , wherein said samples contain DNA, and said DNA is purified.
Claim 9
Dependent Claim
12 words
A method according to claim 7 , wherein said adsorbent is hydroxyapatite.
Claim 10
Dependent Claim
28 words
A method according to claim 3 , 4 , or 7 , wherein said media for treating said samples are salt solutions that strongly interact with said adsorbent.
Claim 11
Dependent Claim
19 words
A method according to claim 9 , wherein the cation component of said salt is ammonium or an amine.
Claim 12
Dependent Claim
18 words
A method according to claim 9 , wherein the anion component of said salt is a volatile acid.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.