EP0288163A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Oligodeoxynucleotides as inhibitors of the replication of retroviruses and the expression of oncogenes
Assignee: United States Department of Commerce
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Filing: 1988-03-24
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Granted: —
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Expires: 2008-03-24
Abstract
Abstract Phosphorothioate oligodeoxyribonucleotide analogs can be used to prevent replication of foreign nucleic acids in the presence of normal living cells, as well as to inhibit the proliferation of neoplastic cells.
Inventors
- 👤 Gerald Zon
- 👤 Kazuo Shinozuka
- 👤 Makoto Matsukura
- 👤 Jack S. Cohen
- 👤 Leonard M. Neckers
- 👤 Shee Loong Loke
- 👤 Cy Aaron Stein
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 09:00
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/70; A61P; A61P31/00; A61P31/12; A61P35/00; C; C07; C07H; C07H21/00; C07H21/04; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1131; C12N15/1132; C12N2310/00; C12N2310/30; C12N2310/31; C12N2310/315; C12N2310/33; C12N2310/335; C12N2310/35; C12N2310/352; C12N2310/3521
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
75 words
A composition for inhibiting the expression of an oncogene said composition comprising a polymer having units of the Formula I: wherein n is an integer of from 2 to 30, B is selected from adenine (A), guanine (G), cytosine (C) and thymine (T), and X is selected from S and 0, with the provision that at least one X in the compound is S, and a specific "anti-sense" sequence, disposed in a pharmaceutically acceptable carrier.
Claim 2
Dependent Claim
75 words
A composition for inhibiting the expression of an oncogene said composition comprising a polymer having units of the Formula I: wherein n is an integer of from 2 to 30, B is selected from adenine (A), guanine (G), cytosine (C) and thymine (T), and X is selected from S and 0, with the provision that at least one X in the compound is S, and a specific "anti-sense" sequence, disposed in a pharmaceutically acceptable carrier.
Claim 3
Dependent Claim
19 words
The composition of Claim 1, wherein the oncogene to be inhibited is an amplified copy of the c-myc gene.
Claim 4
Dependent Claim
20 words
The composition of Claim 1, wherein in two monomers X is S and in the remaining monomers X is O.
Claim 5
Dependent Claim
24 words
The composition of Claim 3, wherein the polymer is end-capped with monomers wherein X is S and in the remaining monomers X is O.
Claim 6
Dependent Claim
24 words
The composition of Claim 1, wherein the monomers wherein X is S and the monomers wherein X is O are present in equal amounts.
Claim 7
Dependent Claim
19 words
A composition for inhibiting replication of a foreign nucleic acid, comprising a phosphorothioate oligodeoxynucleotide in a pharmaceutically acceptable carrier.
Claim 8
Dependent Claim
19 words
A composition for inhibiting replication of a foreign nucleic acid, comprising a phosphorothioate oligodeoxynucleotide in a pharmaceutically acceptable carrier.
Claim 9
Dependent Claim
38 words
The composition according to Claim 6 wherein the phosphorothioate oligodeoxynucleotide has the Formula II: wherein n is an integer of from 2 to 30 and B is selected from adenine (A) guanine (G), cytosine (C) and thymine (T).
Claim 10
Dependent Claim
17 words
The composition according to Claim 6 or 7 wherein the foreign nucleic acid is human immunodeficiency virus.
Claim 11
Dependent Claim
23 words
The composition according to Claim 6 or 7 wherein the oligodeoxyribonucleotide is oligo-dC phosphorothioate having from about 5 mers to about 30 mers.
Claim 12
Dependent Claim
23 words
The composition according to Claim 6 or 7 wherein the oligodeoxynucleotide is oligo-dA phosphorothioate having from about 5 mers to about 30 mers.
Claim 13
Dependent Claim
18 words
The composition according to Claim 6 or 7 wherein the oligodeoxynucleotide is oligo-dA phosphorothioate of about 28 mers.
Claim 14
Dependent Claim
20 words
The composition according to Claim 6 or 7 wherein the carrier is selected from saline, an organic carrier, or blood.
Claim 15
Dependent Claim
35 words
A method for preventing replication and cytophathic effect of foreign nucleic acid in cells in vitro, comprising exposing the cells to a replication-inhibiting amount of an oligodeoxynucleotide of the Formula II + of Claim 7.
Claim 16
Dependent Claim
48 words
A method for preventing replication of foreign nucleic acid in cells in vitro, comprising exposing the cells to a replication-inhibiting amount of an oligodeoxynucleotide of Formula I of Claim 1 wherein the ` oligodeoxynucleotide has a base sequence complementary to a base sequence of human immunodeficiency virus (HIV).
Claim 17
Dependent Claim
15 words
A pharmaceutical composition comprising a compound of Formula I of Claim 1 and a dideoxynucleotide.
Claim 18
Dependent Claim
13 words
The composition according to Claim 7, wherein the dideoxynucleotide is 2', 3 -dideoxyadenosine.
Claim 19
Dependent Claim
29 words
A method of providing compositions capable of selectively inhibiting replication of foreign nucleic acids such as viruses, comprising selectively binding an oligodeoxynucleotide phosphorothioate of Formula II of Claim 7.
Claim 20
Dependent Claim
22 words
A method of Claim 17, wherein the base sequence bound to the oligodeoxynucleotide is complementary to a base sequence of HIV virus.
References Cited (Backward Citations)
No citation records indexed.
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