EP0288082A2
Expired / Lapsed
EP
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A tumor specific assay for CA125 ovarian cancer antigen
Assignee: Research Corp Technologies Inc
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Filing: 1988-04-22
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Granted: —
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Expires: 2008-04-22
Abstract
Abstract The present invention relates to a 40 kilodalton subunit of CA125 antigen, useful in the diagnosis and monitoring of ovarian cancer. It also relates to an immunoassay method for detection of the antigen in serum for diagnosis and monitoring purposes.
Inventors
- 👤 Timothy J. O'brien
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 16:28
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/30; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/811; Y10S436/813; Y10S530/00; Y10S530/808; Y10S530/81
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
13 words
A subunit of CA125 antigen having a molecular weight of about 40 kilodaltons.
Claim 2
Dependent Claim
13 words
A subunit of CA125 antigen having a molecular weight of about 40 kilodaltons.
Claim 3
Dependent Claim
14 words
The subunit of Claim 1 further characterized as being a subunit of tumor-associated CA125.
Claim 4
Dependent Claim
13 words
An antibody having specificity for a 40 kilodalton subunit of the antigen CA125.
Claim 5
Dependent Claim
13 words
An antibody having specificity for a 40 kilodalton subunit of the antigen CA125.
Claim 6
Dependent Claim
10 words
The antibody of Claim 3 which is a monoclonal antibody.
Claim 7
Independent Claim
42 words
A method of detection of ovarian cancer which comprises the steps of contacting the serum of an individual suspected of having the cancer with at least one antibody having specificity for a 40 kilodalton subunit of CA125 and detecting a subunit-antibody reaction.
Claim 8
Independent Claim
42 words
A method of detection of ovarian cancer which comprises the steps of contacting the serum of an individual suspected of having the cancer with at least one antibody having specificity for a 40 kilodalton subunit of CA125 and detecting a subunit-antibody reaction.
Claim 9
Dependent Claim
132 words
The method of Claim 5 which comprises the steps of: a) contacting serum of a patient suspected of having ovarian cancer with a first antibody having specificity for either the 40 kilodalton subunit of CA125 or for CA125 antigen, the first antibody being bound to a solid surface, and allowing time sufficient for formation of a binary complex; b) adding to the binary complex a second antibody having specificity either for the subunit or for CA125 antigen, the second antibody being labeled with a reporter molecule capable of giving a detectable signal, and allowing time sufficient for formation of a ternary complex, at least one of said antibodies having specificity for the 40 kilodalton subunit; c) detecting the presence of the ternary complex by observing the detectable signal of the reporter molecule.
Claim 10
Dependent Claim
78 words
A kit for the diagnosis and monitoring of ovarian cancer, the kit being compartmentalized to receive: a) a first container containing an antibody having specificity for a 40 kilodalton subunit of CA125 antigen; b) a second container containing a second antibody having specificity for CA125 antigen or the 40 kilodalton subunit; one of said antibodies being immobilized on a solid surface and another of said antibodies being labelled with a reporter molecule capable of giving a detectable signal.
Claim 11
Dependent Claim
78 words
A kit for the diagnosis and monitoring of ovarian cancer, the kit being compartmentalized to receive: a) a first container containing an antibody having specificity for a 40 kilodalton subunit of CA125 antigen; b) a second container containing a second antibody having specificity for CA125 antigen or the 40 kilodalton subunit; one of said antibodies being immobilized on a solid surface and another of said antibodies being labelled with a reporter molecule capable of giving a detectable signal.
Claim 12
Dependent Claim
24 words
The kit of Claim 7 wherein the reporter molecule is a radioisotope, an enzyme, a fluorescent molecule, a chemiluminescent molecule or a bioluminescent molecule.
Claim 13
Dependent Claim
12 words
The kit of Claim 7 wherein the reporter molecule is an enzyme.
Claim 14
Dependent Claim
21 words
The kit of Claim 9 wherein the kit further comprises: c) a third container container containing a substrate for the enzyme.
References Cited (Backward Citations)
No citation records indexed.
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