EP0286371A2
Expired / Lapsed
EP
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Immunoassay device
Assignee: Syntex USA LLC
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Filing: 1988-04-06
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Granted: —
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Expires: 2008-04-06
Abstract
Abstract A device is disclosed for conducting an assay method. The device 10 comprises a housing 12, means 18 enclosed in the housing 12 for capturing a member of a specific binding pair in a zone and for allowing liquid to be transported by capillary action away from the zone, one or more self-contained liquid reagents 30 enclosed in the housing 12 for conducting an assay method for the determination of an analyte in the sample, and means 44 in the housing 12 for introducing the sample into the device 10. Preferably, the self-contained reagents 30 are liquid reagents which are contained in a breakable container 32. The device of the invention finds use in assay methods for the determination of an analyte in a sample suspected of containing the analyte. Kits for conducting an asssay method are also disclosed.
Inventors
- 👤 Pyare Khanna
- 👤 Rohan Peries
- 👤 Edwin F. Ullman
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 15:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
115 words
A device for conducting an assay method for the determination of an analyte in a sample, said device comprising: (a) a housing, (b) means enclosed in said housing for capturing a member of a specific binding pair in a zone and for allowing liquid to be transported by capillary action away from said zone, (c) at least one self-contained liquid reagent enclosed in said housing for conducting an assay method for the determination of an analyte in a sample, and (d) means in said housing for introducing the sample into the device, wherein said at least one liquid reagent travels by capillary action to the site of contact of said sample with said means (b).
Claim 2
Independent Claim
115 words
A device for conducting an assay method for the determination of an analyte in a sample, said device comprising: (a) a housing, (b) means enclosed in said housing for capturing a member of a specific binding pair in a zone and for allowing liquid to be transported by capillary action away from said zone, (c) at least one self-contained liquid reagent enclosed in said housing for conducting an assay method for the determination of an analyte in a sample, and (d) means in said housing for introducing the sample into the device, wherein said at least one liquid reagent travels by capillary action to the site of contact of said sample with said means (b).
Claim 3
Dependent Claim
24 words
The device of Claim 1 wherein the means in (b) comprises a bibulous material that is immunosorbing or is capable of being rendered immunosorbing.
Claim 4
Dependent Claim
31 words
The device of Claim 2 wherein said bibulous material has a member of a specific binding pair (sbp) bound thereto, preferably wherein said sbp member is an antibody or an antigen.
Claim 5
Dependent Claim
24 words
The device of Claim 2 or claim 3 wherein a second piece of bibulous material is in liquid receiving relationship with said bibulous material.
Claim 6
Independent Claim
40 words
The device of any one of the preceding claims wherein one or more of said liquid reagents contain members of a signal producing system capable of generating a signal in relation to the amount of an analyte in the sample.
Claim 7
Dependent Claim
71 words
The device of Claim 4 wherein: the first bibulous material has a member of a specific binding pair (sbp) non-diffusively bound thereto, the second bibulous material is in liquid receiving relationship to said first bibulous material, said at least one liquid reagent is contained in a breakable container, said means (d) is an opening in said housing, which device further comprises means integral with said housing allowing for breaking said container.
Claim 8
Independent Claim
19 words
The device of any one of the preceding claims wherein one of said liquid reagents contains an enzyme substrate.
Claim 9
Dependent Claim
117 words
A device of Claim 2 for conducting an assay for a protein in a sample suspected of containing said protein, which device further comprises: a means for viewing the interior of said housing, said bibulous material enclosed in said housing having a specific binding member (sbp) for said protein non-diffusively bound to a situs on said bibulous material opposite said first means for viewing the interior of said housing and also having a labeled sbp member of said protein diffusively deposited on said bibulous material opposite said second means for introducing the sample into the device, or between said first and second means, and wherein said liquid reagent container is breakable and contains therein an aqueous reagent.
Claim 10
Dependent Claim
24 words
A kit for conducting an assay method, said kit comprising in a packaged combination the device of any one of Claims 1 to 8.
Claim 11
Dependent Claim
103 words
A method for conducting an assay for an analyte suspected of being in a sample, which method comprises: (a) introducing a test solution comprising said sample into the device of Claim 1, (b) allowing said test solution to traverse at least a portion of said means for capturing, (c) releasing said at least one self-contained liquid reagent, (d) allowing said at least one released reagent to traverse at least a portion of said means for capturing and at least through said zone, and (e) observing said zone for the presence of a signal in relation to the presence of analyte in said sample.
References Cited (Backward Citations)
No citation records indexed.
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