EP0286367A2
Expired / Lapsed
EP
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Stabilization of beta-galactosidase peptide fragments
Assignee: Microgenics Corp
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Filing: 1988-04-06
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Granted: —
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Expires: 2008-04-06
Abstract
Abstract A method of stabilizing beta -galactosidase peptide fragments against storage degradation for use in a complementation assay which comprises storing the peptide fragment in a storage medium containing an ionic surfactant or a surfactant derived from a sugar residue.
Inventors
- 👤 Pyare L. Khanna
- 👤 Robert Dworschack
- 👤 Phillip Ruprecht
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 22:16
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/581; C; C12; C12Q; C12Q1/00; C12Q1/34; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/924; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/963
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
36 words
A method of stabilizing β-galactosidase peptide fragments against storage loss of complementation activity, which comprises: storing a β-galactosidase peptide fragment in a storage medium containing an ionic surfactant or a surfactant derived from a sugar residue.
Claim 2
Independent Claim
36 words
A method of stabilizing β-galactosidase peptide fragments against storage loss of complementation activity, which comprises: storing a β-galactosidase peptide fragment in a storage medium containing an ionic surfactant or a surfactant derived from a sugar residue.
Claim 3
Dependent Claim
40 words
The method of claim 1, wherein said surfactant is a fatty sulfonate, a fatty acid amide of an amino acid, a fatty acid ester of a sugar or sugar acid amide, or a sulfonate-containing derivative of a cholic acid amide.
Claim 4
Dependent Claim
22 words
The method of claim 2, wherein said surfactant is a salt of dodecyl sulfate, N-lauroylsarcosine, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate, taurodeoxycholic acid, or decanoyl N-methylgluconamide.
Claim 5
Independent Claim
24 words
The method of any one of claims 1, 2 and 3, wherein said surfactant is present at a concentration of from 0.006 to 1.8%.
Claim 6
Independent Claim
15 words
The method of any preceding claim wherein said peptide fragment is a β-galactosidase α-region fragment.
Claim 7
Independent Claim
29 words
The method of any preceding claim which further comprises adding a cyclodextrin to said storage medium after said storing but prior to using said fragment in a complementation assay.
Claim 8
Dependent Claim
17 words
The method of claim 6, wherein said cyclodextrin is α- or β-cyclodextrin and said surfactant is N-lauroylsarcosine.
Claim 9
Dependent Claim
22 words
The method of claim 6, wherein said cyclodextrin is γ-cyclodextrin and said surfactant is a sulfonate-containing derivative of a cholic acid amide.
Claim 10
Independent Claim
22 words
The method of any one of claims 6 to 8 wherein the molar ratio of cyclodextrin to surfactant is at least 1:1.
Claim 11
Independent Claim
17 words
The method of any one of claims 1 to 4 wherein said fragment comprises β-galactosidase residues 6-51.
Claim 12
Dependent Claim
29 words
The method of claim 1, wherein a complementation assay using said stored β-galactosidase peptide fragment is carried out in a solution containing said surfactant and at least 3.3% serum.
Claim 13
Dependent Claim
32 words
A composition containing β-galactosidase peptide fragment, characterised in that it comprises an ionic surfactant or a surfactant derived from a sugar residue, to stabilize the fragment against storage loss of complementation activity.
Claim 14
Dependent Claim
32 words
A composition containing β-galactosidase peptide fragment, characterised in that it comprises an ionic surfactant or a surfactant derived from a sugar residue, to stabilize the fragment against storage loss of complementation activity.
Claim 15
Dependent Claim
41 words
A composition according to claim 12, wherein said surfactant is a fatty sulfonate, a fatty acid amide of an amino acid, a fatty acid ester of a sugar or sugar acid amide, or a sulfonate-containing derivative of a cholic acid amide.
Claim 16
Dependent Claim
24 words
A composition according to claim 13, wherein said surfactant is a salt of dodecyl sulfate, N-lauroylsarcosine, 3-[(3-cholamidopropyl)dimethylammonio] -1-propanesulfonate, 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate, taurodeoxycholic acid, or decanoyl N-methylgluconamide.
Claim 17
Dependent Claim
24 words
A composition according to any one of claims 12 to 14, wherein said surfactant is present at a concentration of from 0.006 to 1.8%.
Claim 18
Dependent Claim
20 words
A composition according to any one of claims 12 to 15, wherein said peptide fragment is a β-galactosidase α-region fragment.
Claim 19
Dependent Claim
18 words
A composition according to any one of claims 12 to 15, wherein said fragment comprises β-galactosidase residues 6-51.
References Cited (Backward Citations)
No citation records indexed.
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