EP0286118A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Glucose electrode and method of determining glucose
Assignee: Nova Biomedical Corp
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Filing: 1988-04-08
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Granted: —
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Expires: 2008-04-08
Abstract
Abstract An electrode assembly for polarographic assay of glucose in solution having a laminated membrane, the outer membrane being from l to 20 m thick and having a pore size of 10 to 125 A, the inner membrane having a thickness of 2 to 4 m, and bonded to the outer membrane with immobilized glucose oxidase. Rapid assays are carried out by measuring the current difference before equilibrium is attained and at least 5 seconds after initial contact of sample with the outer membrane.
Inventors
- 👤 Chung Chang Young
- 👤 Handani Winarta
- 👤 Kenneth Gary
- 👤 Chin-Chun Chen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 05:12
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; C12Q1/006; B; B01; B01J; B01J19/00; B01J19/0093; B01J2219/00; B01J2219/00781; B01J2219/00783; B01J2219/00819; B01J2219/00824; B01J2219/00828; B01J2219/00844; B01J2219/00851; B01J2219/00853; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
119 words
An electrode assembly for use in a polarographic cell for assay of glucose in solution, said assembly comprising a reference electrode and a sensor electrode having a laminated membrane covering the solution-contacting face of said sensor electrode, characterized in that said laminated membrane comprises an outer solution-contacting membrane from l to 20 µ m thick having a pore size of 10 to 125 Å, an inner membrane adjacent the face of said sensor electrode from 2 to 4 µ m thick, and an adhesive glucose oxidase layer between said membranes holding them together, said inner membrane having a permeability sufficiently great to ensure passage of hydrogen peroxide from said glucose oxidase layer to the face of said sensor electrode.
Claim 2
Dependent Claim
119 words
An electrode assembly for use in a polarographic cell for assay of glucose in solution, said assembly comprising a reference electrode and a sensor electrode having a laminated membrane covering the solution-contacting face of said sensor electrode, characterized in that said laminated membrane comprises an outer solution-contacting membrane from l to 20 µ m thick having a pore size of 10 to 125 Å, an inner membrane adjacent the face of said sensor electrode from 2 to 4 µ m thick, and an adhesive glucose oxidase layer between said membranes holding them together, said inner membrane having a permeability sufficiently great to ensure passage of hydrogen peroxide from said glucose oxidase layer to the face of said sensor electrode.
Claim 3
Dependent Claim
17 words
An electrode assembly as claimed in claim 1 further characterized in that said outer membrane comprises polycarbonate.
Claim 4
Dependent Claim
22 words
An electrode assembly as claimed in either of claims 1 or 2 further characterized in that said inner membrane comprises cellulose acetate.
Claim 5
Dependent Claim
67 words
An electrode assembly as claimed in any of claims 1-3 further characterized in comprising, in addition, a flow cell for directing a sample of said solution past the exposed face of said outer electrode, said cell being sealed to said face and having a chamber exposed to said face, and an inlet and an outlet for said chamber, said chamber, inlet and outlet having a fixed volume.
Claim 6
Independent Claim
89 words
The method of assaying glucose in solution characterized in comprising the steps of bringing a sample of said solution into contact with the outer membrane of an electrode assembly as claimed in any of claims 1-4, measuring the current between said electrodes at a time at least as great as 5 seconds after initial contact and substantially less than the time required for the current to become constant, and comparing the measured current with that of a standard solution containing a known concentration of glucose measured under identical conditions.
Claim 7
Dependent Claim
133 words
The method of assaying glucose in solution characterized in comprising the steps of flowing through the flow cell of an electrode assembly as claimed in any of claim 4 a sample of said solution, measuring the maximum current between said electrodes at a time at least as great as 5 seconds after initial contact and substantially less than the time required for the potential to become constant, and comparing the measured maximum current with that of a standard solution containing a known concentration of glucose measured under identical conditions, said sample having a fixed volume greater than the volume of said chamber and inlet, and said rate of flow being such that all of said sample except for residual liquid film is passed through said chamber before said measured current has become constant.
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