EP0281822A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Basic fibroblast growth factor mutein, DNA and its use
Assignee: Takeda Pharmaceutical Co Ltd
•
Filing: 1988-02-20
•
Granted: —
•
Expires: 2008-02-20
Abstract
Abstract A mutein of basic fibroblast growth factors (bFGF) possesses fibroblast growth promoting activity, growth stimulating activity of capillary endothelial cells and angiogenic activity, has high stability and is low toxicity. And therefore, the present mutein is advantageously used as a healing accelerator for burns etc., a therapeutic drug for thrombosis etc., and a cell cultivation promoter.
Inventors
- 👤 Masaharu Senoo
- 👤 Tsutomu Krokawa
- 👤 Koichi Igarashi
- 👤 Reiko Sasada
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 22:36
CPC Classifications
C; C12; C12N; C12N15/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6887; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/50; C07K14/503; C07K14/52; C07K14/54; C07K14/55; C07K16/00; C07K16/18; C07K16/22; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
49 words
A method for producing a mutein of basic fibroblast growth factor (bFGF), which comprises cultivating a transformant harboring a vector containing a recombinant DNA having a base sequence which codes for a mutein of bFGF in a culture medium to produce and accumulate the mutein in the cultured broth.
Claim 2
Independent Claim
49 words
A method for producing a mutein of basic fibroblast growth factor (bFGF), which comprises cultivating a transformant harboring a vector containing a recombinant DNA having a base sequence which codes for a mutein of bFGF in a culture medium to produce and accumulate the mutein in the cultured broth.
Claim 3
Dependent Claim
20 words
A method as claimed in Claim 1, wherein at least one amino acid lacks from the constituent amino acid bFGF.
Claim 4
Dependent Claim
20 words
A method as claimed in Claim 1, wherein at least one constituent amino acid is replaced by other amino acid.
Claim 5
Dependent Claim
19 words
A method as claimed in Claim 1, wherein the bFGF includes the amino acid sequence: Phe-Phe-Leu-Arg-Ile-His-Pro-Asp-Gly-Arg-Val-Asp-Gly-Val-Arg-Glu-Lys-Ser-Asp-Pro in its molecule.
Claim 6
Dependent Claim
19 words
A method as claimed in Claim 3, wherein the constituent amino acid which is to be replaced is cysteine.
Claim 7
Dependent Claim
20 words
A method as claimed in Claim 3, wherein at least one constituent cysteine is replaced by a neutral amino acid.
Claim 8
Dependent Claim
17 words
A method as claimed in Claim 1, wherein at least one constituent cysteine is replaced by serine.
Claim 9
Dependent Claim
25 words
A method as claimed in Claim 3, wherein the constituent amino acid which is to be replaced is aspartic acid, arginine, glycine, serine and/or valine.
Claim 10
Dependent Claim
25 words
A method as claimed in Claim 2, wherein at least one amino acids lacks from the amino terminal of the constituent amino acids of bFGF.
Claim 11
Dependent Claim
25 words
A method as claimed in Claim 2, wherein at least one amino acids lacks from the carboxyl terminal of the constituent amino acids of bFGF.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.