EP0279664A2
Expired / Lapsed
EP
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A method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign gene product thereby
Assignee: Mitsui Chemicals Inc
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Filing: 1988-02-18
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Granted: —
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Expires: 2008-02-18
Abstract
Abstract Escherichia coli carrying a hybrid plasmid constructed by inserting a desired foreign gene into an expression vector, was cultured in a medium containing a sugar component utilizable by the E. coli as a carbon source at a concentration of 0.3% or more so that the expression of said desired foreign gene was suppressed. This E. coli was cultured in the medium in which the sugar concentration was maintained at 0.3% or more in a first process to suppress the foreign gene and to support sufficient cell growth and thereafter at less than 0.3% in a second process to release the suppression to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.
Inventors
- 👤 Nobuhiro Fukuhara
- 👤 Setsuo Yoshino
- 👤 Satori Sone
- 👤 Yoshiyuki Nakajima
- 👤 Nobuyoshi Makiguchi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 17:48
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/67; C12N9/00; C12N9/88
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
79 words
A method of regulating the expression of a foreign gene in a bacterium of Escherichia coli carrying a recombinant plasmid which is constructed by inserting said foreign gene into an expression vector so as to permitting expression of said foreign gene, which comprises a process of maintaining a concentration of a sugar component as a carbon source at 0.3% or more in a medium for culturing said E. coli so as to suppress the expression of said foreign gene.
Claim 2
Independent Claim
79 words
A method of regulating the expression of a foreign gene in a bacterium of Escherichia coli carrying a recombinant plasmid which is constructed by inserting said foreign gene into an expression vector so as to permitting expression of said foreign gene, which comprises a process of maintaining a concentration of a sugar component as a carbon source at 0.3% or more in a medium for culturing said E. coli so as to suppress the expression of said foreign gene.
Claim 3
Dependent Claim
27 words
A method of regulating expression of a foreign gene as set forth in claim 1, wherein said foreign gene is a structure gene encoding for L-phenylalanine ammonialyase.
Claim 4
Dependent Claim
26 words
A method of regulating expression of a foreign gene as set forth in claim 2, wherein said L-phenylalanine ammonialyase has the amino acid sequence as follows:
Claim 5
Dependent Claim
32 words
A method of regulating expression of a foreign gene as set forth in claim 1, wherein said Escherichia coli is Escherichia coli FERM P-9024 carrying the recombinant plasmid pSYP L -3 therein.
Claim 6
Dependent Claim
30 words
A method of regulating expression of a foreign gene as set forth in claim 1, wherein said Escherichia coli is Escherichia coli FERM P-8876 carrying the recombinant plasmid pYtrp6 therein.
Claim 7
Dependent Claim
30 words
A method of regulating expression of a foreign gene as set forth in claim 1, wherein said Escherichia coli is Escherichia coli FERM P-9023 carrying the recombinant plasmid pSW115 therein.
Claim 8
Dependent Claim
118 words
A process of producing a desired foreign gene product by culturing an Escherichia coli carrying a recombinant plasmid which is constructed by inserting said desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene, which comprises steps of (a) a first culture process for culturing said E. coli , in which a concentration of a sugar component as a carbon source in a culture medium is maintained at 0.3% or more and (b) a second culture process for culturing said E. coli grown in the first culture process, in which a concentration of said sugar component in a culture medium for said second culture process is maintained at less than 0.3%.
Claim 9
Dependent Claim
118 words
A process of producing a desired foreign gene product by culturing an Escherichia coli carrying a recombinant plasmid which is constructed by inserting said desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene, which comprises steps of (a) a first culture process for culturing said E. coli , in which a concentration of a sugar component as a carbon source in a culture medium is maintained at 0.3% or more and (b) a second culture process for culturing said E. coli grown in the first culture process, in which a concentration of said sugar component in a culture medium for said second culture process is maintained at less than 0.3%.
Claim 10
Dependent Claim
22 words
A process of producing a foreign gene product as set forth in claim 7, wherein said foreign gene product is L-phenylalanine ammonialyase.
Claim 11
Dependent Claim
27 words
A process of producing a foreign gene product as set forth in claim 8, wherein said L-phenylalanine ammonialyase has the amino acid sequence indicated in claim 3.
Claim 12
Dependent Claim
31 words
A process of producing a foreign gene product as set forth in claim 7, wherein said E. coli is E. coli FERM P-9024 carrying the recombinant plasmid pSYP L -3 therein.
Claim 13
Dependent Claim
29 words
A process of producing a foreign gene product as set forth in claim 7, wherein said E. coli is E. coli FERM P-8876 carrying the recombinant plasmid pYtrp6 therein.
Claim 14
Dependent Claim
29 words
A process of producing a foreign gene product as set forth in claim 7, wherein said E. coli is E. coli FERM P-9023 carrying the recombinant plasmid pSW115 therein.
References Cited (Backward Citations)
No citation records indexed.
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