EP0279069A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method and device for ketone measurement
Assignee: Abbott Laboratories
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Filing: 1987-12-22
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Granted: —
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Expires: 2007-12-22
Abstract
Abstract The present invention relates to methods and materials for the detection of ketone and aldehyde analytes in fluid samples by means of reacting analyte containing samples with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which an amine is covalently coupled. Methods and devices are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject.
Inventors
- 👤 Samar K. Kundu
- 👤 Richard W. George
- 👤 Steven C. March
- 👤 Sangvorn Rutnarak
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 08:57
CPC Classifications
A; A61; A61B; A61B5/00; A61B5/48; A61B5/4869; A61B5/4872; A61B5/08; A61B5/083; G; G01; G01N; G01N31/00; G01N31/22; G01N31/223; G01N33/00; G01N33/48; G01N33/483; G01N33/497; G01N33/50; G01N33/64; G01N33/98; Y; Y10; Y10T; Y10T436/00; Y10T436/20; Y10T436/200833; Y10T436/202499; Y10T436/25; Y10T436/25375; Y10T436/255
Claims Analysis
Total Claims: 52
Claim 1
Independent Claim
75 words
A method for the detection of a ketone or aldehyde analyte in a fluid sample comprising: (a) contacting the analyte present in the sample with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which a primary or secondary amine is covalently coupled, (b) reacting the analyte with the nitroprusside and the amine to form a detectable reaction product, and (c) detecting the reaction product.
Claim 2
Independent Claim
75 words
A method for the detection of a ketone or aldehyde analyte in a fluid sample comprising: (a) contacting the analyte present in the sample with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which a primary or secondary amine is covalently coupled, (b) reacting the analyte with the nitroprusside and the amine to form a detectable reaction product, and (c) detecting the reaction product.
Claim 3
Dependent Claim
13 words
The method according to claim 1 wherein the nitroprusside salt is sodium nitroprusside.
Claim 4
Dependent Claim
13 words
The method according to claim 1 wherein the amine is a primary amine.
Claim 5
Dependent Claim
13 words
The method according to claim 1 wherein the fluid sample is a vapor.
Claim 6
Dependent Claim
11 words
The method according to claim 1 wherein the analyte is acetone.
Claim 7
Dependent Claim
36 words
The method according to claim 1 wherein analytes are adsorbed onto an adsorbant material and are desorbed from the adsorbant material and contacted with the first and second solid matrix materials by means of a solvent.
Claim 8
Dependent Claim
13 words
The method according to claim 6 wherein the solvent comprises methanol and dimethylsulfoxide.
Claim 9
Dependent Claim
23 words
The method according to claim 4 wherein analytes present in the vapor sample are adsorbed onto the first and second solid matrix materials.
Claim 10
Dependent Claim
35 words
The method according to claim 1 wherein the analyte concentration may be determined by detecting the extent to which analytes present in the sample fluid are linearly adsorbed onto the first and second matrix materials.
Claim 11
Independent Claim
43 words
A device for the detection of ketones and aldehydes in a fluid sample comprising a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which a primary or secondary amine compound is covalently bound.
Claim 12
Independent Claim
43 words
A device for the detection of ketones and aldehydes in a fluid sample comprising a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which a primary or secondary amine compound is covalently bound.
Claim 13
Dependent Claim
19 words
The device according to claim 10 wherein said first and second solid matrix materials are immobilized on a strip.
Claim 14
Dependent Claim
19 words
The device according to claim 10 wherein said first and second solid matrix materials are immobilized in a column.
Claim 15
Dependent Claim
13 words
The device according to claim 10 wherein the nitroprusside salt is sodium nitroprusside.
Claim 16
Dependent Claim
13 words
The device according to claim 10 wherein the amine is a primary amine.
Claim 17
Dependent Claim
26 words
The device according to claim 10 wherein said first and second matrix materials are selected from the group consisting of silica gel materials and cellulosic materials.
Claim 18
Dependent Claim
11 words
The device according to claim 10 further comprising a preadsorbant zone.
Claim 19
Dependent Claim
39 words
The device according to claim 10 comprising means for isolation of a fixed volume of vapor in equilibrium with the liquid sample and means for contacting said fixed volume of vapor with the first and second solid matrix materials.
Claim 20
Dependent Claim
21 words
The device according to claim 10 wherein the nitroprusside salt and the amine are coupled to the same solid matrix material.
Claim 21
Dependent Claim
19 words
The device according to claim 10 comprising amino propyl silica gel and sodium nitroprusside coupled to diethylamino silica gel.
Claim 22
Independent Claim
45 words
A method for ascertaining the fat catabolism effects of a weight loss dietary regimen, said method comprising: (a) periodically assaying breath for acetone content, and (b) correlating breath acetone content to a standard reflecting the effect on breath acetone of fixed rates of fat catabolism.
Claim 23
Independent Claim
45 words
A method for ascertaining the fat catabolism effects of a weight loss dietary regimen, said method comprising: (a) periodically assaying breath for acetone content, and (b) correlating breath acetone content to a standard reflecting the effect on breath acetone of fixed rates of fat catabolism.
Claim 24
Dependent Claim
26 words
The method according to claim 20 wherein the period is 24 hours and wherein the fixed rate is expressed in ounces of fat catabolized per day.
Claim 25
Dependent Claim
16 words
The method according to claim 20 wherein the standard is in the form of an equation.
Claim 26
Dependent Claim
13 words
The method according to claim 20 wherein the standard is in tabular form.
Claim 27
Dependent Claim
19 words
The method according to claim 20 wherein the standard is a graphic adjunct to a breath acetone assaying device.
Claim 28
Dependent Claim
106 words
The method for ascertaining the fat catabolism effect of a weight loss dietary regimen according to claim 20 comprising: (a) determining the breath acetone concentration of the subject by (i) contacting a breath sample with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which a primary or secondary amine is covalently coupled, (ii) reacting the acetone with the nitroprusside and amine to form a detectable reaction product, and (iii) detecting the reaction product, and (b) correlating the breath acetone concentration to a standard reflecting the effect on breath acetone of fixed rates of fat catabolism.
Claim 29
Dependent Claim
69 words
A kit for the determination of ketone and aldehyde concentrations in a vapor sample comprising: (a) a first solid matrix material to which a nitroprusside salt is coupled, (b) a second solid matrix material to which a primary or secondary amine is covalently bound, (c) a solvent, and (d) means for collecting a fixed volume of sample vapor and contacting it with the first and second solid matrix materials.
Claim 30
Dependent Claim
69 words
A kit for the determination of ketone and aldehyde concentrations in a vapor sample comprising: (a) a first solid matrix material to which a nitroprusside salt is coupled, (b) a second solid matrix material to which a primary or secondary amine is covalently bound, (c) a solvent, and (d) means for collecting a fixed volume of sample vapor and contacting it with the first and second solid matrix materials.
Claim 31
Dependent Claim
67 words
A kit for the determination of ketone and aldehyde concentrations in a liquid sample comprising: (a) a first solid matrix material to which a nitroprusside salt is coupled, (b) a second solid matrix material to which a primary or secondary amine is covalently bound, and (c) means for collecting a fixed volume of the liquid sample and contacting it with the first and second solid matrix materials.
Claim 32
Dependent Claim
67 words
A kit for the determination of ketone and aldehyde concentrations in a liquid sample comprising: (a) a first solid matrix material to which a nitroprusside salt is coupled, (b) a second solid matrix material to which a primary or secondary amine is covalently bound, and (c) means for collecting a fixed volume of the liquid sample and contacting it with the first and second solid matrix materials.
Claim 33
Dependent Claim
83 words
A kit for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising: (a) a first solid matrix material to which a nitroprusside salt is coupled, (b) a second solid matrix material to which a primary or secondary amine is covalently bound, (c) a solvent, (d) means for collecting a fixed volume of breath and contacting it with the first and second solid matrix materials, and (e) a standard reflecting the effect on breath acetone of fixed rates of fat catabolism.
Claim 34
Dependent Claim
83 words
A kit for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising: (a) a first solid matrix material to which a nitroprusside salt is coupled, (b) a second solid matrix material to which a primary or secondary amine is covalently bound, (c) a solvent, (d) means for collecting a fixed volume of breath and contacting it with the first and second solid matrix materials, and (e) a standard reflecting the effect on breath acetone of fixed rates of fat catabolism.
Claim 35
Independent Claim
153 words
A device for monitoring levels of various components in the breath of a user, comprising: a) an outer tubular member having an open first end and a second end; b) valve means disposed toward the second end of said outer tubular member; c) a breath sample collection means having one end mounted on said tubular member and having the other end closed, and having an interior in communication with the inside of said outer tubular member through said valve means; and d) a disposable tubular analyzer column having a mouthpiece and containing material reactive to the presence of said breath components, wherein said column is insertable into said outer tubular member and co-operates with said valve means to permit breath blown by a user to pass through the outer tubular member into said sample collection means and to permit said collected breath sample to discharge through the reactive material of said analyzer column.
Claim 36
Independent Claim
153 words
A device for monitoring levels of various components in the breath of a user, comprising: a) an outer tubular member having an open first end and a second end; b) valve means disposed toward the second end of said outer tubular member; c) a breath sample collection means having one end mounted on said tubular member and having the other end closed, and having an interior in communication with the inside of said outer tubular member through said valve means; and d) a disposable tubular analyzer column having a mouthpiece and containing material reactive to the presence of said breath components, wherein said column is insertable into said outer tubular member and co-operates with said valve means to permit breath blown by a user to pass through the outer tubular member into said sample collection means and to permit said collected breath sample to discharge through the reactive material of said analyzer column.
Claim 37
Dependent Claim
89 words
The monitoring device as recited in claim 29 wherein said analyzer column is insertable into said outer tubular member to a first rotational and axial position for breath collecting in which breath blown into the mouthpiece bypasses said reactive material and passes through said valve means into said collecting means, said analyzer column being rotatable and further insertable to a second rotational and axial position for breath sample discharging in which a collected breath sample is discharged through said valve means into said reactive material from said collecting means.
Claim 38
Dependent Claim
83 words
The monitoring device as recited in claim 29 wherein the analyzer column further comprises a reduced-diameter central portion creating a passageway between the inside of said outer tubular member and the outside of said analyzer column, said central portion being connected to said mouthpiece by an inclined wall segment having at least one orifice therein such that, when said valve means is in a first position, the breath of a user passes from said mouthpiece through said passageway to said sample collecting means.
Claim 39
Dependent Claim
32 words
The monitoring device as recited in claim 29 wherein longitudinally extending rib means are provided on the inner surface of said outer tubular member for guiding said analyzer column when inserted therein.
Claim 40
Dependent Claim
28 words
The monitoring device as recited in claim 32 wherein positioning flats are provided on said analyzer column for cooperation with said rib means on said outer tubular member.
Claim 41
Dependent Claim
36 words
The monitoring device as recited in claim 32 wherein stop means are provided on at least one of said rib means for engagement by said analyzer column to determine a first axial position of said column.
Claim 42
Dependent Claim
74 words
The monitoring device as recited in claim 30 wherein said valve means comprises a housing member, a ball member disposed therein, an O-ring, and a further reduced-diameter end portion of said analyzer column, said further reduced-diameter end portion of said column being axially spaced from said O-ring in said first position of the column and, in said second position of the column, passing through and sealingly engaging said O-ring and abutting against said ball.
Claim 43
Dependent Claim
78 words
The monitoring device as recited in claim 30 wherein said sample collection means comprises an inflatable/deflatable bag and a flat coil spring is provided on the outer surface of said bag so that, after said bag is inflated and said analyzer column is moved into said second position, said spring coils said bag into its deflated condition with the fixed volume of air being discharged at a substantially constant rate through said reactive material in the analyzer column.
Claim 44
Dependent Claim
56 words
The monitoring device as recited in claim 29 wherein said reactive material includes at least one fluid reactant, said tubular analyzer column has a flexible portion adjacent said reactive material and said outer tubular member includes means for squeezing said flexible portion thereby to break an ampule of said fluid reactant disposed in said flexible portion.
Claim 45
Dependent Claim
60 words
The monitoring device as recited in claim 37 wherein said means for squeezing comprises an outwardly biased but inwardly movable breaker button mounted in a portable housing surrounding said outer tubular member, said breaker button being in alignment with said flexible portion of the disposable analyzer column and having a stem and head for transferring pressure to said flexible portion.
Claim 46
Dependent Claim
29 words
The monitoring device as recited in claim 37 wherein said means for squeezing is axially positioned to break the end of said ampule most closely adjacent said reactive material.
Claim 47
Dependent Claim
118 words
A kit for the determination of levels of various components in a user's breath, said kit comprising: a) a portable housing adapted to receive a disposable analyzer column containing a mouthpiece and reactive materials; b) bag means in communication with said housing for collecting a fixed volume of a user's breath blown through the mouthpiece and discharging it at a substantially constant rate through the reactive materials; and c) valve means disposed in said housing which, in a first position, prevents said fixed volume of breath from contacting said reactive materials and, in a second position, permits contact of said breath with said reactive materials, said valve means being operable by insertion of the column into the housing.
Claim 48
Dependent Claim
118 words
A kit for the determination of levels of various components in a user's breath, said kit comprising: a) a portable housing adapted to receive a disposable analyzer column containing a mouthpiece and reactive materials; b) bag means in communication with said housing for collecting a fixed volume of a user's breath blown through the mouthpiece and discharging it at a substantially constant rate through the reactive materials; and c) valve means disposed in said housing which, in a first position, prevents said fixed volume of breath from contacting said reactive materials and, in a second position, permits contact of said breath with said reactive materials, said valve means being operable by insertion of the column into the housing.
Claim 49
Dependent Claim
100 words
A disposable, tubular analyzer column for use in a kit for monitoring levels of various components in the breath of a user, said analyzer column comprising: a hollow mouthpiece portion of a diameter permitting partial insertion of said analyzer column into a blow tube of said kit; a central portion of reduced-diameter having an inclined wall segment interconnecting said mouthpiece and said reduced-diameter central portions, said central portion housing a volume of reactive material, and said inclined wall segment defining at least one breath passage opening connecting the hollow interior of the mouthpiece with the exterior of the central portion.
Claim 50
Dependent Claim
100 words
A disposable, tubular analyzer column for use in a kit for monitoring levels of various components in the breath of a user, said analyzer column comprising: a hollow mouthpiece portion of a diameter permitting partial insertion of said analyzer column into a blow tube of said kit; a central portion of reduced-diameter having an inclined wall segment interconnecting said mouthpiece and said reduced-diameter central portions, said central portion housing a volume of reactive material, and said inclined wall segment defining at least one breath passage opening connecting the hollow interior of the mouthpiece with the exterior of the central portion.
Claim 51
Dependent Claim
43 words
The analyzer column as recited in claim 41 wherein said central portion also houses a breakable ampule of liquid reactant adjacent said reactive material, said central portion being flexible enough to permit breaking of said ampule by applying pressure to said central portion.
Claim 52
Dependent Claim
56 words
The analyzer column as recited in claim 41 wherein said tubular member is further characterized by an end portion opposite said mouthpiece portion, said end portion having a further reduced-diameter which is operably engageable with valve means of said kit in at least one position of said analyzer column in the blow tube of said kit.
References Cited (Backward Citations)
No citation records indexed.
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