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EP0278577A1 Expired / Lapsed EP ⏱️ 0.0 years remaining

A process for determining thicknesses of layers, application thereof in determining certain interactions and a means for carrying out this process

Assignee: Akzo NV
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Filing: 1988-02-09
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Granted: —
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Expires: 2008-02-09
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Abstract

Abstract A process for determining the thickness and/or change in thickness of a thin layer applied to a carrier, with the carrier coated with the thin layer being radiated with a plane polarized light beam and the reflectance on carrier and layer being detected. The light reflected by the carrier and the thin layer is split into a p-polarized part and an s-polarized part, after which the respective reflection values R p and R s are measured simultane­ously for the two parts, and the quantity is determined, in which formula m is a variable parameter and the quantity M is a function of the thickness of the thin layer. By measuring both the reflection of p-polarized radiation and that of s-polarized radiation, and by adroitly combining the measuring data obtained, the resulting signal will be insensitive to the intensity of the radiation source. An additional advantage is that the process has a greater sensitivity to variations in layer thickness, and that it is possible for the process to be carried out within a wide linear measuring range. In determining the growth of a protein layer the measuring result is indepen­dent -within wide limits- of the thickness and the index of refraction of a layer previously applied to the carrier.

Inventors

  • 👤 Gerrit Cornelis Dubbeldam

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-06 11:05
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/21; G01N21/211; G01B; G01B11/00; G01B11/02; G01B11/06; G01B11/0616; G01B11/0641; G01B11/065

Claims Analysis

Total Claims: 7
Claim 1
Independent Claim 130 words
A process for determining the thickness and/or change in thickness of a thin layer applied to a carrier, with the carrier coated with the thin layer being radiated with a plane polarized light beam and the reflectance on carrier and layer being detected, after which the measured amount of light detected is converted into a layer thickness and/or change in layer thickness, characterized in that the light reflected by the carrier and the thin layer is split into a p-polarized part and an s-polarized part, after which the respective reflection values R p and R s are measured simultaneously for the two parts, and the quantity is determined, in which formula m is a variable parameter and the quan­tity M is a function of the thickness of the thin layer.
Claim 2
Dependent Claim 130 words
A process for determining the thickness and/or change in thickness of a thin layer applied to a carrier, with the carrier coated with the thin layer being radiated with a plane polarized light beam and the reflectance on carrier and layer being detected, after which the measured amount of light detected is converted into a layer thickness and/or change in layer thickness, characterized in that the light reflected by the carrier and the thin layer is split into a p-polarized part and an s-polarized part, after which the respective reflection values R p and R s are measured simultaneously for the two parts, and the quantity is determined, in which formula m is a variable parameter and the quan­tity M is a function of the thickness of the thin layer.
Claim 3
Dependent Claim 73 words
A process according to claim 1, characterized in that use is made, for determining the degree of growth of organic layers on a carrier, of a carrier previously coated with a thin layer of a transparent inorganic material or of an organic polymer of such a thickness that there is an almost linear relation between the quantity M and the thickness of the organic layer built up on the previously applied thin layer.
Claim 4
Dependent Claim 59 words
A process according to claim 2, characterized in that a carrier is used which is coated with a thin layer of transparent inorganic material or of an organic polymer of such a thickness that there is an almost linear relation between the quantity M and the thickness of the organic layer built up on the previously applied thin layer.
Claim 5
Dependent Claim 56 words
A process according to any one of the claims 1-3, characterized in that the carrier used is a prism, with the light being directed to the prism in such a way that it will enter and leave through the respective lateral faces and be reflected on the base face on which the thin layer is present.
Claim 6
Dependent Claim 33 words
A process for determining protein/carrier and protein/protein inter­actions, characterized in that the process according to any one of the claims 1-4 is applied a number of times using, in succession, different protein solutions.
Claim 7
Dependent Claim 44 words
A means for carrying out the process according to claim 5, comprising a plate-shaped or prism-shaped carrier having one surface coated with a thin layer of a transparent inorganic material or with an organic polymer material of a thickness of at least 40 nm.

References Cited (Backward Citations)

US2666355A Cited
EP0067921A1 Cited
GB2153071A Cited

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