EP0276138A2
Expired / Lapsed
EP
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Chromatographic supports for separation of oligonucleotides
Assignee: EIDP Inc
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Filing: 1988-01-20
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Granted: —
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Expires: 2008-01-20
Abstract
Abstract A chromatographic packing useful in the separation of oligonucleotides comprises an inert, preferably porous, support particle having bonded thereto a silane having a weak anion exchange group in close proximity to at least one polar non-ionic group.
Inventors
- 👤 Geoffrey Bryon Cox
- 👤 Peter Andrew David Edwardson
- 👤 Anthony Atkinson
- 👤 Michael Dennis Scawen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 05:31
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/281; B01J20/286; B01J20/288; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/32; B01D15/325; B01D15/36; B01D15/361; B01J20/28; B01J20/28002; B01J20/28004; B01J20/30; B01J20/3071; B01J20/3078; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3206; B01J20/3208; B01J20/321; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3257; B01J20/3259; B01J20/3263; B01J20/3285; B01J41/00; B01J41/04; B01J41/07; B01J41/20; B01J2220/00; B01J2220/50; B01J2220/54; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2993; Y10T428/2995; Y10T428/2996; Y10T428/2998
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
29 words
A packing for use in chromatographic separation comprising inert support particles having bonded thereto a silane having a weak anion exchange group and at least one polar non-ionic group.
Claim 2
Dependent Claim
29 words
A packing for use in chromatographic separation comprising inert support particles having bonded thereto a silane having a weak anion exchange group and at least one polar non-ionic group.
Claim 3
Dependent Claim
17 words
A packing according to claim 1 wherein the support particles comprise silica, alumina or an organic polymer.
Claim 4
Dependent Claim
21 words
A packing according to claim 1 or 2 wherein the support particles are from 2 µm to 30 µm in diameter.
Claim 5
Dependent Claim
29 words
A packing according to claim 1, 2 or 3 wherein the weak anion exchange group is a secondary amine group and the polar non-ionic group is a hydroxyl group.
Claim 6
Dependent Claim
17 words
A packing according to any one of the preceding claims wherein the bonded silane has hydrophobic regions.
Claim 7
Dependent Claim
31 words
A packing according to claim 4 or 5 wherein the bonded silane comprises a group of the formula Si-CH₂-CH₂-CH₂-O-CH₂- -CH₂-NH-(CH₂) n -OH where n is an integer from 2 to 6.
Claim 8
Dependent Claim
13 words
A packing according to claim 6 wherein the group is of the formula
Claim 9
Dependent Claim
30 words
A packing according to claim 4 or 5 wherein the bonded silane comprises a group of the formula where n is 1 or 2 and X is alkyl or hydroxyalkyl.
Claim 10
Dependent Claim
23 words
A packing according to any one of the preceding claims wherein the silane surface coverage of the particles is more than 0.9 µmole/m².
Claim 11
Independent Claim
51 words
A method of separating oligonucleotides which comprises entraining the oligonucleotides in a mobile liquid phase and chromatographically subjecting the oligonucleotides to a packing as claimed in any one of the preceding claims by passing the mobile phase through the packing, thereby using both reversed-phase and ion-exchange mechanisms to effect the separation.
Claim 12
Dependent Claim
17 words
A method according to claim 10 wherein the mobile phase has a pH from 4.0 to 8.5.
Claim 13
Independent Claim
63 words
A method of formulating a chromatographic packing for use in chromatographically separating oligonucleotides, which comprises either reacting a reactive silane with a modifying reagent containing a weak anion exchange group and a polar non-ionic group to provide a modified silane, and then reacting the modified silane with a support, or reacting the reactive silane with the support and then with the modifying reagent.
Claim 14
Independent Claim
63 words
A method of formulating a chromatographic packing for use in chromatographically separating oligonucleotides, which comprises either reacting a reactive silane with a modifying reagent containing a weak anion exchange group and a polar non-ionic group to provide a modified silane, and then reacting the modified silane with a support, or reacting the reactive silane with the support and then with the modifying reagent.
Claim 15
Dependent Claim
26 words
A method according to claim 12 wherein the modifying reagent is of the formula NH₂-(CH₂) n -OH, where n is an integer from 2 to 6.
Claim 16
Dependent Claim
20 words
A method according to claim 12 wherein the reactive silane is γ-glycidoxy proopyltrimethoxyl silane and the modifying agent is 2-aminoethanol.
Claim 17
Dependent Claim
31 words
A method according to claim 12 wherein the modifying reagent is of the formula NH₂- -(CH₂OH) n H (2-n) where n is 1 or 2 and X is alkyl or hydroxyalkyl.
References Cited (Backward Citations)
No citation records indexed.
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