EP0274394A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Chimeric antibody with specificity to human B cell surface antigen
Assignee: Oncogen LP
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Filing: 1988-01-07
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Granted: —
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Expires: 2008-01-07
Abstract
Abstract A chimeric antibody with human constant region and murine variable region, having specificity to a 35 kDA polypeptide (Bp35(CD20)) expressed on the surface of human B cells, methods of production, and uses.
Inventors
- 👤 Randy R. Robinson
- 👤 Alvin Y. Liu
- 👤 Karl Erik Hellstrom
- 👤 Ingegerd Hellstrom
- 👤 Jeffrey A. Ledbetter
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 14:56
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/2887; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/68; A61K47/6835; A61K47/6849; A61K47/6875; A61K47/6877; C07K16/2896; C07K16/46; C07K16/461; C07K16/462; A61K39/00; A61K2039/505; A61K38/00; C07K2317/00; C07K2317/20; C07K2317/24; C07K2317/70; C07K2317/73; C07K2317/732; C07K2317/734; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/30; C07K2319/40
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
30 words
A polynucleotide molecule comprising a cDNA sequence coding for the variable region of an immunoglobulin chain having specificity to a 35-kDa polypeptide (Bp35(CD20)) expressed on the surface of B cells.
Claim 2
Dependent Claim
30 words
A polynucleotide molecule comprising a cDNA sequence coding for the variable region of an immunoglobulin chain having specificity to a 35-kDa polypeptide (Bp35(CD20)) expressed on the surface of B cells.
Claim 3
Dependent Claim
12 words
The molecule of claim l wherein said chain is a heavy chain.
Claim 4
Dependent Claim
12 words
The molecule of claim l wherein said chain is a light chain.
Claim 5
Dependent Claim
30 words
The molecule of claim l which further comprises an additional sequence coding for the constant region of a human immunoglobulin chain, both said sequences in operable linkage with each other.
Claim 6
Dependent Claim
13 words
The molecule of claim 4 wherein said additional sequence is a cDNA sequence.
Claim 7
Dependent Claim
13 words
The molecule of claim 4 wherein said additional sequence is a genomic sequence.
Claim 8
Dependent Claim
11 words
The molecule of claim l which is a recombinant DNA molecule.
Claim 9
Dependent Claim
11 words
The molecule of claim 7 which is in double-stranded DNA form.
Claim 10
Dependent Claim
10 words
The molecule of claim 7 which is an expressible vehicle.
Claim 11
Dependent Claim
12 words
l0. The molecule of claim 9 wherein said vehicle is a plasmid.
Claim 12
Dependent Claim
10 words
A prokaryotic host transformed with the molecule of claim 4.
Claim 13
Dependent Claim
9 words
The host of claim ll which is a bacterium.
Claim 14
Dependent Claim
10 words
A eukaryotic host transfected with the molecule of claim 4.
Claim 15
Dependent Claim
12 words
The host of claim l3 which is yeast or a mammalian cell.
Claim 16
Dependent Claim
29 words
A heavy immunoglobulin chain comprising a constant human region and a variable region having specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells.
Claim 17
Dependent Claim
29 words
A heavy immunoglobulin chain comprising a constant human region and a variable region having specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells.
Claim 18
Dependent Claim
29 words
A light immunoglobulin chain comprising a constant human region and a variable region having specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells.
Claim 19
Dependent Claim
29 words
A light immunoglobulin chain comprising a constant human region and a variable region having specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells.
Claim 20
Dependent Claim
41 words
A chimeric antibody molecule comprising two light chains and two heavy chains, each of said chains comprising a constant human region and a variable region having specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells.
Claim 21
Dependent Claim
41 words
A chimeric antibody molecule comprising two light chains and two heavy chains, each of said chains comprising a constant human region and a variable region having specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells.
Claim 22
Dependent Claim
9 words
The antibody of claim l7 in detectably labelled form.
Claim 23
Dependent Claim
11 words
The antibody of claim l7 immobilized on an aqueous-insoluble solid phase.
Claim 24
Dependent Claim
54 words
A process of preparing an immunoglobulin heavy chain having a constant human region and a variable region having specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells which comprises: culturing a host capable of expressing said chain under culturing conditions and recovering from said culture said heavy chain.
Claim 25
Dependent Claim
54 words
A process of preparing an immunoglobulin heavy chain having a constant human region and a variable region having specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells which comprises: culturing a host capable of expressing said chain under culturing conditions and recovering from said culture said heavy chain.
Claim 26
Dependent Claim
55 words
2l. A process of preparing an immunoglobulin light chain having a constant human region and a variable region with specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells which comprises: culturing a host capable of expressing said chain under culturing conditions; and recovering from said culture said light chain.
Claim 27
Dependent Claim
55 words
2l. A process of preparing an immunoglobulin light chain having a constant human region and a variable region with specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells which comprises: culturing a host capable of expressing said chain under culturing conditions; and recovering from said culture said light chain.
Claim 28
Dependent Claim
76 words
A process of preparing a chimeric immunoglobulin containing a heavy chain and a light chain, each of said heavy and light chains having a constant human region and a variable region with specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells which comprises: culturing a host capable of expressing said heavy chain, or said light chain, or both, under culturing conditions; and recovering from said culture said chimeric immunoglobulin molecule.
Claim 29
Dependent Claim
76 words
A process of preparing a chimeric immunoglobulin containing a heavy chain and a light chain, each of said heavy and light chains having a constant human region and a variable region with specificity to a 35 kDa polypeptide (Bp35(CD20)) expressed on the surface of human B cells which comprises: culturing a host capable of expressing said heavy chain, or said light chain, or both, under culturing conditions; and recovering from said culture said chimeric immunoglobulin molecule.
Claim 30
Dependent Claim
15 words
The process of any of claims 20, 2l or 22 wherein said host is prokaryotic.
Claim 31
Dependent Claim
15 words
The process of any of claims 20, 2l or 22 wherein said host is eukaryotic.
Claim 32
Dependent Claim
42 words
An immunoassay method for the detection of a 35 kDa polypeptide normally expressed on the surface of B cells in a sample, which comprises: contacting said sample with the antibody of claim l7 and detecting whether said antibody binds to said antigen.
Claim 33
Dependent Claim
41 words
An in vivo or in vitro imaging method to detect an antigen comprising a 35 kDa polypeptide normally expressed on the surface of B cells which comprises contacting said antigen with the labelled antibody of claim l8 and detecting said antibody.
Claim 34
Independent Claim
35 words
A method of killing cells carrying an antigen thereon, which antigen comprising a 35 kDa polypeptide normally expressed on the surface of B cells which comprises: contacting said cells with the antibody of claim l7.
Claim 35
Dependent Claim
16 words
The method of claim 27 wherein said killing occurs by complement mediated lysis of said cells.
Claim 36
Dependent Claim
11 words
The method of claim 27 wherein said killing occurs by ADCC.
References Cited (Backward Citations)
No citation records indexed.
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