EP0271988A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Anti-sense regulation of plant gene expression
Assignee: Syngenta Ltd
•
Filing: 1987-11-06
•
Granted: —
•
Expires: 2007-11-06
Abstract
Abstract Recombinant DNA comprises promoter and terminator base sequences respectively upstream and downstream of an inverted base sequence complementary to a substantial sequence of bases in polygalacturonase mRNA. The antisense mRNA produced thereby delays softening of fruit, in particular tomatoes.
Inventors
- 👤 Ian George Bridges
- 👤 Wolfgang Walter Schuch
- 👤 Donald Grierson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 08:44
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1137; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8242; C12N15/8243; C12N15/8249; C12N9/00; C12N9/14; C12N9/16; C12N9/18; C12N9/24; C12N9/2402; C12Y; C12Y301/00; C12Y301/01; C12Y301/01011; C12Y302/00; C12Y302/01; C12Y302/01015; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
58 words
Recombinant DNA comprising an upstream promoter base sequence, a base sequence for transcription in the mRNA under control of said upstream promoter base sequence, and a downstream transcription terminator base sequence, characterised in that the base sequence for transcription comprises an inverted sequence of bases complementary to a substantial run of bases in mRNAs encoding fruit softening enzymes.
Claim 2
Dependent Claim
58 words
Recombinant DNA comprising an upstream promoter base sequence, a base sequence for transcription in the mRNA under control of said upstream promoter base sequence, and a downstream transcription terminator base sequence, characterised in that the base sequence for transcription comprises an inverted sequence of bases complementary to a substantial run of bases in mRNAs encoding fruit softening enzymes.
Claim 3
Dependent Claim
17 words
DNA as claimed in claim 1 in which the fruit softening enzyme is polygalacturonase or pectin esterase.
Claim 4
Dependent Claim
20 words
DNA as claimed in claim 2 in which the base sequence for transcription is at least 50 bases in length.
Claim 5
Dependent Claim
34 words
DNA as claimed in either of claims 2 or 3 in which the base sequence for transcription comprises a sequence of bases complementary to a part of the sequence set forth in Figure 1.
Claim 6
Dependent Claim
36 words
DNA as claimed in any of claims 2 to 4 in which the base sequence for transcription comprises a base sequence complementary to the sequence of bases set forth in Figure 1 from 2 to 730.
Claim 7
Dependent Claim
36 words
DNA as claimed in any of claims 2 to 4 in which the base sequence for transcription comprises a base sequence complementary to the sequence of bases set forth in Figure 1 from 2 to 197.
Claim 8
Dependent Claim
24 words
DNA as claimed in any of claims 2 to 6 in which the upstream promoter sequence is a constitutive promoter such as CaMV 35S.
Claim 9
Dependent Claim
19 words
DNA as claimed in any of claims 2 to 6 in which the promoter is the PG gene promoter.
Claim 10
Dependent Claim
13 words
A vector containing DNA as claimed in any of claims 1 to 8.
Claim 11
Dependent Claim
13 words
Plant cells containing DNA as claimed in any of claims 1 to 8.
Claim 12
Dependent Claim
9 words
Dicotyledonous plants composed of cells claimed in claim 10.
Claim 13
Dependent Claim
10 words
Plants as claimed in claim 11 which are tomato plants.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.