EP0268772A2
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EP
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Expression of biologically active factor XIII
Assignee: University of Washington
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Filing: 1987-09-18
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Granted: —
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Expires: 2007-09-18
Abstract
Abstract DNA sequences that code for proteins having substantially the same biological activity as Factor XIII are disclosed. DNA sequence coding for polypeptides that are functionally homologous to the a, aʹ, and b subunits of Factor XIII are contained within expression vectors useful for transfecting a variety of eucaryotic and procaryotic host cells. Methods for preparing (a) proteins having substantially the same biological activity as Factor XIII; (b) proteins which, upon dissociation in the presence of calcium, have substantially the same biological activity as Factor XIIIa; (c) proteins which, upon actuation, have substantially the same biological activity as Factor XIIIa; and (d) methods for preparing proteins which have substantially the same biological activity of Factor XIIIa are also disclosed.
Inventors
- 👤 Earl W. Davie
- 👤 Ronald L. Seale
- 👤 Akitada Ichinose
- 👤 Julie Ann Holly
- 👤 Gary E. Parker
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-27 11:03
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/10; C12N9/1025; C12N9/104; C12N9/1044; A; A61; A61P; A61P7/00; A61P7/04; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/625; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/644; C12Y; C12Y304/00; C12Y304/21; C12Y304/21022; A61K; A61K38/00; C07; C07K; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/036
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
21 words
A DNA sequence that codes for a polypeptide that is functionally homologous to the a or aʹ subunit of Factor XIII.
Claim 2
Dependent Claim
21 words
A DNA sequence that codes for a polypeptide that is functionally homologous to the a or aʹ subunit of Factor XIII.
Claim 3
Dependent Claim
25 words
The DNA sequence of claim 1 comprising the sequence of Figure 1, from bp 91 to bp 2283 or from bp 202 to bp 2283.
Claim 4
Dependent Claim
42 words
The DNA sequence of claim 1 comprising a DNA sequence coding for the amino acid sequence of Figure 1, starting with serine, number 1, and ending eith methionine, number 731 or starting with glycine, number 38, and ending with methionine, number 731.
Claim 5
Dependent Claim
19 words
A DNA sequence that codes for a polypeptide that is functionally homologous to the b subunit of Factor XIII.
Claim 6
Dependent Claim
19 words
A DNA sequence that codes for a polypeptide that is functionally homologous to the b subunit of Factor XIII.
Claim 7
Dependent Claim
22 words
The DNA sequence of claim 4 wherein said DNA sequence comprises the sequence of Figure 2, from bp 59 to bp 1981.
Claim 8
Dependent Claim
30 words
The DNA sequence of claim 4 comprising a DNA sequence coding for the amino acid sequence of Figure 2, starting the glutamate, number 1, and ending with threonine, number 641.
Claim 9
Dependent Claim
28 words
An expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a protein having substantially the same biological activity as human Factor XIII.
Claim 10
Dependent Claim
28 words
An expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a protein having substantially the same biological activity as human Factor XIII.
Claim 11
Dependent Claim
19 words
An expression vector comprising a transcriptional promoter followed downstream by a DNA sequence according to any of claims 1-6.
Claim 12
Dependent Claim
19 words
The expression vector of claims 7 or 8 wherein said promoter is the ADH2-46 TPI1 , or GAL10 .
Claim 13
Dependent Claim
14 words
A host cell transfected with an expression vector according to any of claims 7-9.
Claim 14
Dependent Claim
15 words
The host cell of claim 10 wherein said host cell is a yeast host cell.
Claim 15
Independent Claim
94 words
A method of preparing a protein which, upon activation, has substantially the same biological activity as Factor XIIIa, comprising: introducing into a host cell an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the a subunit of Factor XIII and a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the b subunit of Factor XIII; culturing said host cell under suitable conditions; and isolating the protein from said host cell.
Claim 16
Independent Claim
94 words
A method of preparing a protein which, upon activation, has substantially the same biological activity as Factor XIIIa, comprising: introducing into a host cell an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the a subunit of Factor XIII and a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the b subunit of Factor XIII; culturing said host cell under suitable conditions; and isolating the protein from said host cell.
Claim 17
Independent Claim
94 words
A method of preparing a protein which, upon activation, has substantially the same biological activity as Factor XIIIa, comprising: introducing into a host cell an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the aʹ subunit of Factor XIII and a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the b subunit of Factor XIII; culturing said host cell under suitable conditions; and isolating the protein from said host cell.
Claim 18
Independent Claim
94 words
A method of preparing a protein which, upon activation, has substantially the same biological activity as Factor XIIIa, comprising: introducing into a host cell an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the aʹ subunit of Factor XIII and a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the b subunit of Factor XIII; culturing said host cell under suitable conditions; and isolating the protein from said host cell.
Claim 19
Independent Claim
77 words
A method of preparing a protein which, upon activation, has substantially the same biological activity as Factor XIIIa, comprising: introducing into host cells an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the a subunit of Factor XIII; culturing said host cells under suitable conditions; isolating the a subunits from said host cells; and dimerizing the a subunits to form an a₂ homodimer.
Claim 20
Independent Claim
77 words
A method of preparing a protein which, upon activation, has substantially the same biological activity as Factor XIIIa, comprising: introducing into host cells an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the a subunit of Factor XIII; culturing said host cells under suitable conditions; isolating the a subunits from said host cells; and dimerizing the a subunits to form an a₂ homodimer.
Claim 21
Independent Claim
68 words
A method of preparing a protein which, upon activation, has substantially the same biological activity as Factor XIIIa, comprising: introducing into a host cell an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the a subunit of Factor XIII; culturing said host cell under suitable conditions; and isolating a₂ homodimers from said host cell.
Claim 22
Independent Claim
68 words
A method of preparing a protein which, upon activation, has substantially the same biological activity as Factor XIIIa, comprising: introducing into a host cell an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the a subunit of Factor XIII; culturing said host cell under suitable conditions; and isolating a₂ homodimers from said host cell.
Claim 23
Independent Claim
74 words
A method of preparing a protein which has substantially the same biological activity as Factor XIIIa, comprising: introducing into host cells an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the aʹ subunit of Factor XIII; culturing said host cells under suitable conditions; isolating the aʹ subunits from said host cells; and dimerizing the aʹ subunits to form aʹ₂ homodimers.
Claim 24
Independent Claim
74 words
A method of preparing a protein which has substantially the same biological activity as Factor XIIIa, comprising: introducing into host cells an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the aʹ subunit of Factor XIII; culturing said host cells under suitable conditions; isolating the aʹ subunits from said host cells; and dimerizing the aʹ subunits to form aʹ₂ homodimers.
Claim 25
Independent Claim
66 words
A method of preparing a protein which has substantially the same biological activity as Factor XIIIa, comprising: introducing into a host cell an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the aʹ subunit of Factor XIII; culturing said host cell under suitable conditions; and isolating aʹ₂ homodimer from said host cell.
Claim 26
Independent Claim
66 words
A method of preparing a protein which has substantially the same biological activity as Factor XIIIa, comprising: introducing into a host cell an expression vector comprising a transcriptional promoter followed downstream by a DNA sequence that codes for a polypeptide that is functionally homologous to the aʹ subunit of Factor XIII; culturing said host cell under suitable conditions; and isolating aʹ₂ homodimer from said host cell.
Claim 27
Dependent Claim
14 words
A protein produced according to any of the methods set forth in claims 12-17.
Claim 28
Dependent Claim
24 words
A pharmaceutical composition comprising an effective amount of a protein produced according to any of claims 12-17, and a physiologically acceptable carrier or diluent.
Claim 29
Dependent Claim
16 words
A protein produced according to any of claims 12-17, for use as an active therapeutic substance.
References Cited (Backward Citations)
No citation records indexed.
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