EP0264819A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Variant aequorin genes and process for producing variant aequorin proteins
Assignee: JNC Corp
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Filing: 1987-10-14
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Granted: —
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Expires: 2007-10-14
Abstract
Abstract Various variants of photoprotein aequorin (pAQ440), useful for elucidating the mechanism of its luminescence and thereby extending the possibility of concrete applications of aequorin protein, and a process for producing variant aequorin proteins and provided, which variants are obtained by converting base(s) in a specified order of the base arrangement of aequorin gene into other base(s), or by deleting a certain bases in specified continued orders thereof, according to site-specific mutagenesis method.
Inventors
- 👤 Satoshi Inouye
- 👤 Kouichi Kurose
- 👤 Yoshiyuki Sakaki
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 09:02
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43595
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
199 words
In the following base arrangement of pAQ440 as aequorin gene: variants having converted a base or bases indicated in the following items (i) to (xiii) into other definite base or bases, or having deleted bases indicated therein: (i) a variant having converted the 220th base G into C; (ii) a variant having converted the 238th base G into A; (iii) a variant having converted the 307th base C into T, and also the 308th base A into T; (iv) a variant having converted the 499th base G into C; (v) a variant having converted the 568th base T into C; (vi) a variant having converted the 569th base G into C; (vii) a variant having converted the 590th base G into C; (viii) a variant having converted the 607th base G into C; (ix) a variant having converted the 625th base G into A; (x) a variant having converted the 616th base G into C, and also the 625th base G into A; (xi) a variant having converted the 674th base G into C; (xii) a variant having deleted the 205th to the 207th bases GAT; and (xiii) a variant having deleted the 592nd to the 594th bases GAT.
Claim 2
Dependent Claim
199 words
In the following base arrangement of pAQ440 as aequorin gene: variants having converted a base or bases indicated in the following items (i) to (xiii) into other definite base or bases, or having deleted bases indicated therein: (i) a variant having converted the 220th base G into C; (ii) a variant having converted the 238th base G into A; (iii) a variant having converted the 307th base C into T, and also the 308th base A into T; (iv) a variant having converted the 499th base G into C; (v) a variant having converted the 568th base T into C; (vi) a variant having converted the 569th base G into C; (vii) a variant having converted the 590th base G into C; (viii) a variant having converted the 607th base G into C; (ix) a variant having converted the 625th base G into A; (x) a variant having converted the 616th base G into C, and also the 625th base G into A; (xi) a variant having converted the 674th base G into C; (xii) a variant having deleted the 205th to the 207th bases GAT; and (xiii) a variant having deleted the 592nd to the 594th bases GAT.
Claim 3
Dependent Claim
210 words
In the following base arrangement of pAQ440 as aequorin gene: a process for producing a variant aequorin protein which comprises using variants having converted a base or bases indicated in the following items (i) to (xii) into other definite base or bases, or having deleted bases indicated therein: (i) a variant having converted the 220th base G into C; (ii) a variant having converted the 238th base G into A; (iii) a variant having converted the 307th base C into T, and also the 308th base A into T; (iv) a variant having converted the 499th base G into C; (v) a variant having converted the 568th base T into C; (vi) a variant having converted the 569th base G into C; (vii) a variant having converted the 590th base G into C; (viii) a variant having converted the 607th base G into C; (ix) a variant having converted the 625th base G into A; (x) a variant having converted the 616th base G into C, and also the 625th base G into A; (xi) a variant having converted the 674th base G into C; (xii) a variant having deleted the 205th to the 207th bases GAT; and (xiii) a variant having deleted the 592nd to the 594th bases GAT.
Claim 4
Dependent Claim
210 words
In the following base arrangement of pAQ440 as aequorin gene: a process for producing a variant aequorin protein which comprises using variants having converted a base or bases indicated in the following items (i) to (xii) into other definite base or bases, or having deleted bases indicated therein: (i) a variant having converted the 220th base G into C; (ii) a variant having converted the 238th base G into A; (iii) a variant having converted the 307th base C into T, and also the 308th base A into T; (iv) a variant having converted the 499th base G into C; (v) a variant having converted the 568th base T into C; (vi) a variant having converted the 569th base G into C; (vii) a variant having converted the 590th base G into C; (viii) a variant having converted the 607th base G into C; (ix) a variant having converted the 625th base G into A; (x) a variant having converted the 616th base G into C, and also the 625th base G into A; (xi) a variant having converted the 674th base G into C; (xii) a variant having deleted the 205th to the 207th bases GAT; and (xiii) a variant having deleted the 592nd to the 594th bases GAT.
Claim 5
Dependent Claim
108 words
In the following base arrangement of pAQ440 as aequorin gene: variants having converted a base or bases indicated in the following items (i) to (iv) into other definite base or bases indicated therein: (i) a variant having converted the 659th base G into C and the 590th base G into C; (ii) a variant having converted the 590th G into C and the 674th G into C; (iii) a variant having converted the 674th G into C and the 569th G into C; and (iv) a variant having converted the 569th base G into C, the 590th base G into C and the 674th base G into C.
Claim 6
Dependent Claim
108 words
In the following base arrangement of pAQ440 as aequorin gene: variants having converted a base or bases indicated in the following items (i) to (iv) into other definite base or bases indicated therein: (i) a variant having converted the 659th base G into C and the 590th base G into C; (ii) a variant having converted the 590th G into C and the 674th G into C; (iii) a variant having converted the 674th G into C and the 569th G into C; and (iv) a variant having converted the 569th base G into C, the 590th base G into C and the 674th base G into C.
Claim 7
Dependent Claim
119 words
In the following base arrangement of pAQ440 as aequorin gene: a process for producing a variant aequorin protein which comprising using variants having converted a base or bases indicated in the following items (i) to (iv) into other definite base or bases indicated therein: (i) a variant having converted the 659th base G into C and the 590th base G into C; (ii) a variant having converted the 590th G into C and the 674th G into C; (iii) a variant having converted the 674th G into C and the 569th G into C; and (iv) a variant having converted the 569th base G into C, the 590th base G into C and the 674th base G into C.
Claim 8
Dependent Claim
119 words
In the following base arrangement of pAQ440 as aequorin gene: a process for producing a variant aequorin protein which comprising using variants having converted a base or bases indicated in the following items (i) to (iv) into other definite base or bases indicated therein: (i) a variant having converted the 659th base G into C and the 590th base G into C; (ii) a variant having converted the 590th G into C and the 674th G into C; (iii) a variant having converted the 674th G into C and the 569th G into C; and (iv) a variant having converted the 569th base G into C, the 590th base G into C and the 674th base G into C.
References Cited (Backward Citations)
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