EP0263934A1
Expired / Lapsed
EP
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Method for separation and purification of biopolymers by affinity chromatography
Assignee: Diagen Institut fur Molekularbiologische Diagnostik GmbH
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Filing: 1987-08-05
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Granted: —
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Expires: 2007-08-05
Abstract
Abstract The method for the separation and purification of biopolymers by means of affinity chromatography using porous silica gel having affinity ligands covalently bonded to the surface thereof is improved by using silica gels which have defined narrow pore size ranges five to twenty times the size of the material to be purified.
Inventors
- 👤 Metin Dr. Colpan
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 07:58
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C07K1/22; B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01J; B01J20/00; B01J20/02; B01J20/10; B01J20/103; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28054; B01J20/28078; B01J20/281; B01J20/286; B01J20/291; B01J20/30; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3257; B01J20/3259; B01J20/3261; B01J20/3263; C07K16/00; C07K16/06; C07K16/065; B01D15/3809; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
64 words
Method for the separation and purification of biopolymers by means of affinity chromatography using porous silica gel, porous glass, kieselgur, aluminumoxide, titaniumoxide, hydroxylapatite, zirconiumoxide or mixtures thereof having affinity ligands covalently bonded to the surface thereof, characterized in that silica gels are used which have defined narrow pore size ranges which have five to twenty times the size of the material to be purified.
Claim 2
Dependent Claim
64 words
Method for the separation and purification of biopolymers by means of affinity chromatography using porous silica gel, porous glass, kieselgur, aluminumoxide, titaniumoxide, hydroxylapatite, zirconiumoxide or mixtures thereof having affinity ligands covalently bonded to the surface thereof, characterized in that silica gels are used which have defined narrow pore size ranges which have five to twenty times the size of the material to be purified.
Claim 3
Dependent Claim
29 words
The method according to claim 1, characterized in that silica gels are used which have defined narrow pore size ranges within a range of from 50 to 1,000 nm.
Claim 4
Dependent Claim
15 words
The method according to claim 1, characterized in that antibodies are used as affinity ligands.
Claim 5
Dependent Claim
21 words
The method according to claim 1, characterized in that the material to be purified is a protein, glycoprotein and/or nucleic acid.
Claim 6
Dependent Claim
19 words
The method according to claim 1, characterized in that the material to be purified is a virus or vaccine.
Claim 7
Dependent Claim
19 words
The method according to claim 1 , characterized in that the material to be purified is a cell organelle.
Claim 8
Dependent Claim
20 words
The method according to claim 1, characterized in that the material to be purified is a prokaryotic or eukaryotic cells.
Claim 9
Dependent Claim
24 words
The method according to claim 1, characterized in that the material to be purified is a micelle, vesicle, chylomicron, VLDL, LDL and/or HDL lipoprotein.
References Cited (Backward Citations)
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