EP0261642A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method for detecting a component of a biological system and detection device and a kit therefor
Assignee: Teijin Ltd
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Filing: 1987-09-22
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Granted: —
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Expires: 2007-09-22
Abstract
Abstract A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), with a solution presumed to contain the biological component to be detected, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Inventors
- 👤 Takeyuki Kawaguchi
- 👤 Takashi Shiro
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 16:27
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/75; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; G01N33/53; G01N33/543; G01N33/54366; G01N21/17; G01N21/25; G01N21/29; G01N21/55; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 40
Claim 1
Independent Claim
103 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), with a solution containing the biological component to be detected, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 2
Independent Claim
103 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), with a solution containing the biological component to be detected, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 3
Independent Claim
144 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), with a solution containing the biological component to be detected, further reacting another substance capable of reacting with another reaction site in the biological component to be detected after or while reacting the detecting substance with the biological component to subject the surface layer of the device to contrast enhancement treatment, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 4
Independent Claim
144 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), with a solution containing the biological component to be detected, further reacting another substance capable of reacting with another reaction site in the biological component to be detected after or while reacting the detecting substance with the biological component to subject the surface layer of the device to contrast enhancement treatment, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 5
Independent Claim
113 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II) and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), with a solution containing the biological component to be detected, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 6
Independent Claim
113 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II) and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), with a solution containing the biological component to be detected, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 7
Independent Claim
154 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II) and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), with a solution containing the biological component to be detected, further reacting another substance capable of reacting with another reaction site in the biological component to be detected after or while reacting the detecting substance with the biological component to subject the surface layer of the device to contrast enhancement treatment, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 8
Independent Claim
154 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II) and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), with a solution containing the biological component to be detected, further reacting another substance capable of reacting with another reaction site in the biological component to be detected after or while reacting the detecting substance with the biological component to subject the surface layer of the device to contrast enhancement treatment, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 9
Independent Claim
112 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II) and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), with a solution containing the biological component to be detected, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 10
Independent Claim
112 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II) and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), with a solution containing the biological component to be detected, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 11
Independent Claim
153 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II) and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), with a solution containing the biological component to be detected, further reacting another substance capable of reacting with another reaction site in the biological component to be detected after or while reacting the detecting substance with the biological component to subject the surface layer of the device to contrast enhancement treatment, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 12
Independent Claim
153 words
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II) and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), with a solution containing the biological component to be detected, further reacting another substance capable of reacting with another reaction site in the biological component to be detected after or while reacting the detecting substance with the biological component to subject the surface layer of the device to contrast enhancement treatment, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claim 13
Dependent Claim
41 words
The method of claim 2, 4 or 6 wherein the other substance used for the contrast enhancement treatment is a secondary antibody, an enzyme-labelled secondary antibody, a secondary antibody fixed to an emulsion, or a secondary antibody fixed to a latex.
Claim 14
Independent Claim
34 words
The method of any one of claims 1 to 6 wherein the substance in the biological component detecting substance layer (III) is an antibody, and the biological component to be detected is an antigen.
Claim 15
Independent Claim
34 words
The method of any one of claims 1 to 6 wherein the substance in the biological component detecting substance layer (III) is an antigen, and the biological component to be detected is an antibody.
Claim 16
Independent Claim
50 words
The method of any one of claims 1 to 6 wherein the substance in the biological component detecting substance layer (III) and the biological component to be detected are the same antigen, and an antibody added to the solution containing an antigen to be detected reacts competitively with both antigens.
Claim 17
Independent Claim
29 words
The method of any one of claims 1 to 6 wherein the light-transmitting reflecting layer (IV) is a layer of a metal formed by a colloidal particle coating method.
Claim 18
Independent Claim
36 words
The method of any one of claims 1 to 6 wherein the light reflectivity of the substrate is high such that the reflection on the substrate is balanced on the reflection of the light-transmitting reflecting layer.
Claim 19
Dependent Claim
57 words
The method of claim 11 wherein the light-transmitting reflecting layer (IV) is a layer of metal colloid particles coated at a pH which is up to 1.0 higher than the isoelectric point of the biological component to be detected and lower than, or more than 1.0 higher than, the isoelectric point of the biological component detecting substance.
Claim 20
Independent Claim
29 words
The method of any one of claims 1 to 6 wherein the light-transmitting reflecting layer (IV) is a layer of a metal formed by a physical vapor deposition method.
Claim 21
Independent Claim
13 words
The method of claims 11, 13 or 14 wherein the metal is gold.
Claim 22
Independent Claim
66 words
The method of any one of claims 1 to 7 wherein the interference color is detected by a color difference value calculated in accordance with the equation X o , Y o and Z o are tristimulus values of an illuminating light source, and X, Y and Z are tristimulus values of the spectrum of the calorimetric standard observer stipulated by International Committee of Illumination (1931).
Claim 23
Dependent Claim
85 words
A kit for detection of a component of a biological system, comprising (1) a pack of a device for detecting the biological component composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), and (2) a pack of a metal colloid, and optionally (3) a pack of a contrast enhancement agent.
Claim 24
Dependent Claim
85 words
A kit for detection of a component of a biological system, comprising (1) a pack of a device for detecting the biological component composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), and (2) a pack of a metal colloid, and optionally (3) a pack of a contrast enhancement agent.
Claim 25
Dependent Claim
95 words
A kit for detection of a component of a biological system, comprising (1) a pack of a device for detecting the biological component composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), and (2) a pack of a metal colloid, and optionally (3) a pack of a contrast enhancement agent.
Claim 26
Dependent Claim
95 words
A kit for detection of a component of a biological system, comprising (1) a pack of a device for detecting the biological component composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), and (2) a pack of a metal colloid, and optionally (3) a pack of a contrast enhancement agent.
Claim 27
Dependent Claim
94 words
A kit for detection of a component of a biological system, comprising (1) a pack of a device for detecting the biological component composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), and (2) a pack of a metal colloid, and optionally (3) a pack of a contrast enhancement agent.
Claim 28
Dependent Claim
94 words
A kit for detection of a component of a biological system, comprising (1) a pack of a device for detecting the biological component composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), and (2) a pack of a metal colloid, and optionally (3) a pack of a contrast enhancement agent.
Claim 29
Independent Claim
53 words
A device for detecting a component of a biological system, comprising a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II).
Claim 30
Independent Claim
53 words
A device for detecting a component of a biological system, comprising a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II).
Claim 31
Independent Claim
63 words
A device for detecting a component of a biological system, comprising a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P).
Claim 32
Independent Claim
63 words
A device for detecting a component of a biological system, comprising a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P).
Claim 33
Independent Claim
15 words
A device for detecting a component of a biological system, comprising a light reflecting substrate
Claim 34
Independent Claim
15 words
A device for detecting a component of a biological system, comprising a light reflecting substrate
Claim 35
Dependent Claim
47 words
(I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P).
Claim 36
Dependent Claim
47 words
(I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P).
Claim 37
Dependent Claim
22 words
The device of claim 20, 21 or 22 wherein the substance in the biological component detecting substance layer (III) is an antibody.
Claim 38
Dependent Claim
22 words
The device of claim 20, 21 or 22 wherein the substance in the biological component detecting substance layer (III) is an antigen.
Claim 39
Dependent Claim
72 words
The device of claim 20, 21 or 22 wherein the light interference layer (II) is a layer of an inorganic substance, and the biological component detecting substance layer (III) is a layer of an antibody protein which is formed by forming a complex of the antibody protein with a substantially water-insoluble mono- or bi-molecular film, and laminating it to the light interference layer whose surface is optionally subjected to a hydrophobizing treatment.
Claim 40
Dependent Claim
131 words
The device of claim 20, 21 or 22 wherein the light interference layer (II) is a layer of an inorganic substance, and the biological component detecting substance layer (III) is a layer of an antibody protein which is formed by spreading a monomolecular film of a long-chain fatty acid having 24 to 32 carbon atoms, a polyvalent metal salt thereof and/or an ester thereof, or a monomolecular film of a polyvalent metal salt of a long-chain fatty acid having 14 to 23 carbon atoms and/or an ester of said fatty acid on a water phase, contacting the film with a water-soluble antibody protein dissolved in the water phase to form an antibody protein-monomolecular film complex on the interface of the aqueous phase, and laminating it onto the light interference layer (II).
References Cited (Backward Citations)
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