EP0260829A2
Expired / Lapsed
EP
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Antibodies reactive with chlorinated phenols and methods of preparing and using same
Assignee: Westinghouse Electric Corp
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Filing: 1987-08-26
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Granted: —
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Expires: 2007-08-26
Abstract
Abstract Monoclonal antibodies that react with chlorinated phenols, particularly, pentachlorophenol, and hybridomas that produce such antibodies are disclosed. Also a method of producing such antibodies by producing an immunogenic conjugate of a chlorinated phenol and a macromolecule carrier, immunizing an animal with the conjugate, obtaining antibody-producing cells from the animal, fusing the cells with tumor cells to produce hybridomas, selecting from among the hydridomas at least one that produces antibodies reactive with the chlorinated phenol, and recovering the antibodies. In addition, polyclonal antibodies to chlorinated phenols and methods of making such antibodies. Also analytic, diagnostic, investigational, separatory, and other methods of using the antibodies, and compositions containing the antibodies for such uses.
Inventors
- 👤 Kenneth W. Hunter
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 08:11
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/44
Claims Analysis
Total Claims: 58
Claim 1
Independent Claim
72 words
A method of producing monoclonal antibodies reactive with a chlorinated phenol characterized by producing an immunogenic conjugate of said chlorinated phenol and a macromolecule carrier; immunizing an animal with said conjugate; obtaining antibody-producing cells from said animal; fusing said cells with tumor cells to produce hybridomas; selecting from among said hybridomas at least one hybridoma that produces antibodies reactive with said chlorinated phenol; and recovering said produced antibodies from said selected hybridoma.
Claim 2
Independent Claim
72 words
A method of producing monoclonal antibodies reactive with a chlorinated phenol characterized by producing an immunogenic conjugate of said chlorinated phenol and a macromolecule carrier; immunizing an animal with said conjugate; obtaining antibody-producing cells from said animal; fusing said cells with tumor cells to produce hybridomas; selecting from among said hybridomas at least one hybridoma that produces antibodies reactive with said chlorinated phenol; and recovering said produced antibodies from said selected hybridoma.
Claim 3
Dependent Claim
14 words
A method according to claim 1, characterized in that the chlorinated phenol is pentachlorophenol.
Claim 4
Dependent Claim
17 words
A method according to claim 1 or 2, characterized in that the immunized animal is a rodent.
Claim 5
Dependent Claim
21 words
A method of claim 1, 2 or 3, characterized in that the tumor cells are mouse plasmacytoma or rat myeloma cells.
Claim 6
Independent Claim
31 words
A method according to any of claims 1 to 4, characterized in that the hybridomas have the characteristics of ATCC HB 9197 or ATCC HB 9198 or mutants or variants thereof.
Claim 7
Independent Claim
114 words
A method of producing monoclonal antibodies reactive with pentachlorophenol characterized by producing an immunogenic conjugate of said pentachlorophenol and a protein; immunizing a mouse with said conjugate; recovering antibody-producing cells from the spleen of said mouse; fusing said antibody-producing cells with mouse plasmacytoma or rat myeloma cells deficient in the enzyme hypoxanthine-guanine phosphoribosyl transferase to form hybridomas; selecting at least one of said hybridomas by growth in a medium comprising hypoxanthine, aminopterin, and thymidine; identifying at least one of said hybridomas that produces an antibody to said pentachlorophenol in free or unconjugated form; culturing said identified hybridoma to produce said antibody in a recoverable quantity; and recovering the antibodies produced by said cultured hybridoma.
Claim 8
Independent Claim
114 words
A method of producing monoclonal antibodies reactive with pentachlorophenol characterized by producing an immunogenic conjugate of said pentachlorophenol and a protein; immunizing a mouse with said conjugate; recovering antibody-producing cells from the spleen of said mouse; fusing said antibody-producing cells with mouse plasmacytoma or rat myeloma cells deficient in the enzyme hypoxanthine-guanine phosphoribosyl transferase to form hybridomas; selecting at least one of said hybridomas by growth in a medium comprising hypoxanthine, aminopterin, and thymidine; identifying at least one of said hybridomas that produces an antibody to said pentachlorophenol in free or unconjugated form; culturing said identified hybridoma to produce said antibody in a recoverable quantity; and recovering the antibodies produced by said cultured hybridoma.
Claim 9
Independent Claim
40 words
A method of producing monoclonal antibodies to pentachlorophenol characterized by culturing a hybridoma having the characteristics of ATCC HB 9197 or ATCC HB 9198 or mutants or variants thereof in a culture medium and recovering said antibodies from said medium.
Claim 10
Independent Claim
40 words
A method of producing monoclonal antibodies to pentachlorophenol characterized by culturing a hybridoma having the characteristics of ATCC HB 9197 or ATCC HB 9198 or mutants or variants thereof in a culture medium and recovering said antibodies from said medium.
Claim 11
Dependent Claim
41 words
An in vivo method of producing monoclonal antibodies reactive with a chlorinated phenol characterized by intraperitoneally placing in a histocompatible or immunosuppressed host a hybridoma that produces said antibodies; and recovering the produced antibodies from the ascites fluid of said host.
Claim 12
Dependent Claim
41 words
An in vivo method of producing monoclonal antibodies reactive with a chlorinated phenol characterized by intraperitoneally placing in a histocompatible or immunosuppressed host a hybridoma that produces said antibodies; and recovering the produced antibodies from the ascites fluid of said host.
Claim 13
Dependent Claim
14 words
A method according to claim 8, characterized in that the chlorinated phenol is pentachlorophenol.
Claim 14
Dependent Claim
16 words
A method according to claim 8 or 9, characterized in that the host is a rodent.
Claim 15
Dependent Claim
30 words
A method according to claim 8, 9 or 10, characterized in that the hybridoma has the characteristics of ATCC HB 9197 or ATCC HB 9198 or mutants or variants thereof.
Claim 16
Independent Claim
78 words
A method for producing a continuous cell line that produces monoclonal antibodies to a chlorinated phenol which comprises: producing an immunogenic conjugate of said chlorinated phenol and a macromolecule carrier; immunizing an animal with said conjugate; obtaining antibody-producing cells from said animal; fusing said antibody-producing cells with tumor cells to produce hybridomas; selecting from among said hybridomas a hybridoma that produces antibodies reactive with said chlorinated phenol; and clonally expanding said selected hybridoma into a continuous cell line.
Claim 17
Independent Claim
78 words
A method for producing a continuous cell line that produces monoclonal antibodies to a chlorinated phenol which comprises: producing an immunogenic conjugate of said chlorinated phenol and a macromolecule carrier; immunizing an animal with said conjugate; obtaining antibody-producing cells from said animal; fusing said antibody-producing cells with tumor cells to produce hybridomas; selecting from among said hybridomas a hybridoma that produces antibodies reactive with said chlorinated phenol; and clonally expanding said selected hybridoma into a continuous cell line.
Claim 18
Dependent Claim
14 words
A method according to claim 12, characterized in that the chlorinated phenol is pentachlorophenol.
Claim 19
Dependent Claim
18 words
A hybridoma which produces a monoclonal antibody characterized in that said antibody is reactive with a chlorinated phenol.
Claim 20
Dependent Claim
18 words
A hybridoma which produces a monoclonal antibody characterized in that said antibody is reactive with a chlorinated phenol.
Claim 21
Dependent Claim
14 words
A hybridoma according to claim 14, characterized in that the chlorinated phenol is pentachlorophenol.
Claim 22
Dependent Claim
29 words
A hybridoma according to claim 14 or 15, characterized in that said hybridoma has the characteristics of ATCC HB 9197 or ATCC HB 9198 or mutants or variants thereof.
Claim 23
Dependent Claim
16 words
A monoclonal or polyclonal antibody characterized in that said antibody is reactive with a chlorinated phenol.
Claim 24
Dependent Claim
16 words
A monoclonal or polyclonal antibody characterized in that said antibody is reactive with a chlorinated phenol.
Claim 25
Dependent Claim
14 words
An antibody according to claim 17, characterized in that the chlorinated phenol is pentachlorophenol.
Claim 26
Dependent Claim
23 words
A monoclonal antibody characterized in that said antibody is recovered from the cell line produced by the method of claim 12 or 13.
Claim 27
Dependent Claim
19 words
An antibody according to claim 17 or 18, characterized in that the monoclonal antibody is recovered from a hybridoma.
Claim 28
Dependent Claim
27 words
An antibody according to claim 20, characterized in that the hybridoma has the characteristics of ATCC HB 9197 or ATCC HB 9198 or mutants or variants thereof.
Claim 29
Independent Claim
36 words
A method for determining the presence or concentration of a chlorinated phenol in a sample by employing an immunoassay, characterized by using a monoclonal antibody reactive with said chlorinated phenol as a reagent in said immunoassay.
Claim 30
Independent Claim
36 words
A method for determining the presence or concentration of a chlorinated phenol in a sample by employing an immunoassay, characterized by using a monoclonal antibody reactive with said chlorinated phenol as a reagent in said immunoassay.
Claim 31
Dependent Claim
16 words
A method according to claim 22, characterized in that the monoclonal antibody is reactive with pentachlorophenol.
Claim 32
Independent Claim
51 words
A method for detecting the presence or concentration of a chlorinated phenol in a sample characterized by adding monoclonal antibodies reactive with said chlorinated phenol to said sample; and determining the presence or concentration of said chlorinated phenol by an immunoassay in which said monoclonal antibodies are used as a reagent.
Claim 33
Independent Claim
51 words
A method for detecting the presence or concentration of a chlorinated phenol in a sample characterized by adding monoclonal antibodies reactive with said chlorinated phenol to said sample; and determining the presence or concentration of said chlorinated phenol by an immunoassay in which said monoclonal antibodies are used as a reagent.
Claim 34
Dependent Claim
37 words
An immunological procedure for the isolation or removal of a chlorinated phenol from a mixture on the basis of a selective immunological reaction with an antibody specific for said chlorinated phenol, characterized by using a monoclonal antibody.
Claim 35
Dependent Claim
37 words
An immunological procedure for the isolation or removal of a chlorinated phenol from a mixture on the basis of a selective immunological reaction with an antibody specific for said chlorinated phenol, characterized by using a monoclonal antibody.
Claim 36
Independent Claim
61 words
A method for the purification of a chlorinated phenol characterized by contacting material containing said chlorinated phenol with immobilized monoclonal antibodies reactive with said chlorinated phenol to separate the latter from said material by forming immobilized complexes of said antibody and said chlorinated phenol; and separating said chlorinated phenol from said immobilized antibodies to recover said chlorinated phenol in purified form.
Claim 37
Independent Claim
61 words
A method for the purification of a chlorinated phenol characterized by contacting material containing said chlorinated phenol with immobilized monoclonal antibodies reactive with said chlorinated phenol to separate the latter from said material by forming immobilized complexes of said antibody and said chlorinated phenol; and separating said chlorinated phenol from said immobilized antibodies to recover said chlorinated phenol in purified form.
Claim 38
Independent Claim
36 words
A method for the biochemical, immunological, functional, or other investigational analysis of a chlorinated phenol, characterized by using a monoclonal antibody reactive with said chlorinated phenol and in an amount effective to result in said analysis.
Claim 39
Independent Claim
36 words
A method for the biochemical, immunological, functional, or other investigational analysis of a chlorinated phenol, characterized by using a monoclonal antibody reactive with said chlorinated phenol and in an amount effective to result in said analysis.
Claim 40
Dependent Claim
38 words
A composition for determining the presence or concentration of a chlorinated phenol in a sample characterized in that said composition is an effective amount of a monoclonal antibody reactive with said chlorinated phenol and in an acceptable carrier.
Claim 41
Dependent Claim
38 words
A composition for determining the presence or concentration of a chlorinated phenol in a sample characterized in that said composition is an effective amount of a monoclonal antibody reactive with said chlorinated phenol and in an acceptable carrier.
Claim 42
Dependent Claim
14 words
A composition according to claim 28, characterized in that the chlorinated phenol is pentachlorophenol.
Claim 43
Dependent Claim
47 words
A composition for isolating or removing a chlorinated phenol from a mixture containing said chlorinated phenol characterized in that said composition is an effective amount of a monoclonal antibody reactive with said chlorinated phenol and immobilized on an acceptable matrix or in admixture with an acceptable carrier.
Claim 44
Dependent Claim
47 words
A composition for isolating or removing a chlorinated phenol from a mixture containing said chlorinated phenol characterized in that said composition is an effective amount of a monoclonal antibody reactive with said chlorinated phenol and immobilized on an acceptable matrix or in admixture with an acceptable carrier.
Claim 45
Dependent Claim
31 words
A composition for the purification of a chlorinated phenol characterized in that said composition is a monoclonal antibody reactive with said chlorinated phenol and immobilized on an acceptable support or carrier.
Claim 46
Dependent Claim
31 words
A composition for the purification of a chlorinated phenol characterized in that said composition is a monoclonal antibody reactive with said chlorinated phenol and immobilized on an acceptable support or carrier.
Claim 47
Dependent Claim
38 words
A composition for the biochemical, immunological, functional, or other investigational analysis of a chlorinated phenol characterized in that said composition is an effective amount of a monoclonal antibody reactive with said chlorinated phenol and in an acceptable carrier.
Claim 48
Dependent Claim
38 words
A composition for the biochemical, immunological, functional, or other investigational analysis of a chlorinated phenol characterized in that said composition is an effective amount of a monoclonal antibody reactive with said chlorinated phenol and in an acceptable carrier.
Claim 49
Independent Claim
49 words
A method of producing polyclonal antibodies reactive with a chlorinated phenol characterized by producing an immunogenic conjugate of said chlorinated phenol and a macromolecule carrier; immunizing an animal with said conjugate; removing blood from said animal; separating the serum from said blood; and recovering the antibodies from said serum.
Claim 50
Independent Claim
49 words
A method of producing polyclonal antibodies reactive with a chlorinated phenol characterized by producing an immunogenic conjugate of said chlorinated phenol and a macromolecule carrier; immunizing an animal with said conjugate; removing blood from said animal; separating the serum from said blood; and recovering the antibodies from said serum.
Claim 51
Dependent Claim
14 words
A method according to claim 33, characterized in that the chlorinated phenol is pentachlorophenol.
Claim 52
Independent Claim
36 words
A method for determining the presence or concentration of a chlorinated phenol in a sample by employing an immunoassay, characterized by using a polyclonal antibody reactive with said chlorinated phenol as a reagent in said immunoassay.
Claim 53
Independent Claim
36 words
A method for determining the presence or concentration of a chlorinated phenol in a sample by employing an immunoassay, characterized by using a polyclonal antibody reactive with said chlorinated phenol as a reagent in said immunoassay.
Claim 54
Independent Claim
50 words
A method of preparing an immunogenic conjugate of a chlorinated phenol and a macromolecule carrier characterized by covalently attaching a chemical linker to said macromolecule carrier; and covalently attaching a derivative of said chlorinated phenol, said derivative containing a reactive group, to said chemical linker, thereby forming said immunogenic conjugate.
Claim 55
Independent Claim
50 words
A method of preparing an immunogenic conjugate of a chlorinated phenol and a macromolecule carrier characterized by covalently attaching a chemical linker to said macromolecule carrier; and covalently attaching a derivative of said chlorinated phenol, said derivative containing a reactive group, to said chemical linker, thereby forming said immunogenic conjugate.
Claim 56
Dependent Claim
14 words
A method according to claim 36, characterized in that the chlorinated phenol is pentachlorophenol.
Claim 57
Dependent Claim
18 words
A synthetic antigen characterized in that said composition is a chlorinated phenol covalently bonded to a macromolecule carrier.
Claim 58
Dependent Claim
18 words
A synthetic antigen characterized in that said composition is a chlorinated phenol covalently bonded to a macromolecule carrier.
References Cited (Backward Citations)
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