EP0257758A2
Expired / Lapsed
EP
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Stable biologically active fluorochemical emulsions
Assignee: 3M Co
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Filing: 1987-07-10
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Granted: —
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Expires: 2007-07-10
Abstract
Abstract Fluorochemical emulsions comprised of a fluorochemical droplet discontinuous phase and aqueous continuous phase with at least one specific binding species immobilized on the fluorochemical droplets are shown. The emulsions may include a primer material to couple to specific binding species to the fluorochemical droplets. The emulsions may be used in diagnostic procedures or biochemical reactors where binding of the immobilized specific binding species to its binding partner is desired. The droplets may also incorporate a species which is detectable by spectrophotometric, fluorometric or colormetric means or a precursor to a detectable species.
Inventors
- 👤 Dean S. Milbrath
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 21:30
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/585; G01N33/53; G01N33/531; G01N33/532; G01N33/543; G01N33/544; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2984
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
63 words
A support for a specific binding reaction comprised of an emulsion having an aqueous continuous phase and a fluorochemical droplet discontinuous phase wherein the fluorochemical droplets have immobilized on their surface at least one specific binding species, the emulsion is capable of contacting the binding partner of the specific binding species and the specific binding species is not bovine serum albumin or lecithin.
Claim 2
Dependent Claim
63 words
A support for a specific binding reaction comprised of an emulsion having an aqueous continuous phase and a fluorochemical droplet discontinuous phase wherein the fluorochemical droplets have immobilized on their surface at least one specific binding species, the emulsion is capable of contacting the binding partner of the specific binding species and the specific binding species is not bovine serum albumin or lecithin.
Claim 3
Dependent Claim
98 words
The biologically active emulsion of claim 1 wherein the specific binding species is selected from the group consisting of one member of an antigen/antibody pair, where the antigen is a naturally occurring or synthetic protein, peptide, polysaccharide, lipid, nucleic acid, organic polymer, antigenic fragment of these materials, an infectious agent, a hapten; lectin; a segment of DNA or RNA; an enzyme; a cell surface marker, a cell surface receptor; a therapeutic substance; a metabolite of a thereapeutic substance; an endotoxin; a specific binding partner of any of the foregoing and combinations and derivatives of any of the foregoing.
Claim 4
Dependent Claim
31 words
The biologically active emulsion of claim 1 wherein the specific binding species is immobilized on the fluorochemical droplets by adsorption at the interface of the continuous phase and the discontinuous phase.
Claim 5
Dependent Claim
40 words
The biologically active emulsion of claim 1 wherein the fluorochemical droplet discontinuous phase further comprises a primer material and the specific binding species is conjugated to the primer material at the interface of the continuous phase and the discontinuous phase.
Claim 6
Dependent Claim
48 words
The biologically active emulsion of claim 4 wherein the primer material is selected from the group consisting of naturally occurring and synthetic polymers with amine, carboxyl, metcapto, or other functional groups capable of specific reactions with coupling agents, and naturally occurring and synthetic polymers which are highly charged.
Claim 7
Dependent Claim
34 words
The biologically active emulsion of claim 5 wherein the primer material is selected from the group consisting of albumins, casein, whole sera, fibrinogen, collagens, and naturally occurring and synthetic poly(amino acids) and charged polysaccharides.
Claim 8
Dependent Claim
32 words
The biologically active emulsion of claim 1 wherein the fluorochemical droplet discontinuous phase is dyed with a colored substance that is soluble in the fluorochemical and insoluble in the aqueous continuous phase.
Claim 9
Dependent Claim
21 words
An emulsion comprised of an aqueous continuous phase and a fluorochemical droplet discontinuous phase wherein the fluorochemical droplets include a dye.
Claim 10
Dependent Claim
21 words
An emulsion comprised of an aqueous continuous phase and a fluorochemical droplet discontinuous phase wherein the fluorochemical droplets include a dye.
Claim 11
Dependent Claim
67 words
An in vitro specific binding process comprising: a. providing in vitro an emulsion having an aqueous continuous phase, a fluorochemical droplet discontinuous phase, and a specific binding species immobilized on the fluorochemical droplets; and b. contacting the fluorochemical droplets and immobilized specific binding species with an aqueous solution containing the specific binding partner of the specific binding species for a period of time sufficient to permit binding.
Claim 12
Dependent Claim
67 words
An in vitro specific binding process comprising: a. providing in vitro an emulsion having an aqueous continuous phase, a fluorochemical droplet discontinuous phase, and a specific binding species immobilized on the fluorochemical droplets; and b. contacting the fluorochemical droplets and immobilized specific binding species with an aqueous solution containing the specific binding partner of the specific binding species for a period of time sufficient to permit binding.
References Cited (Backward Citations)
No citation records indexed.
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