Patent US5124701A successfully refreshed from Google Patents.
EP0256751A2
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Transformation, somatic embryogenesis and whole plant regeneration method for glycine species
Assignee: Mycogen Plant Science Inc
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Filing: 1987-08-04
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Granted: —
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Expires: 2007-08-04
Abstract
Abstract A method for somatic embryogenesis of soybean, ( Glycine max ), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/l. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly embryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed. Methods for transforming somatic tissue from soybean and other Glycine species are also provided. Whole, fertile, transformed plants are obtained.
Inventors
- 👤 Glenn B. Collins
- 👤 David F. Hildebrand
- 👤 Paul A. Lazzeri
- 👤 Thomas R. Adams
- 👤 Wayne A. Parrott
- 👤 Lynn M. Hartweck
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 15:14
CPC Classifications
A; A01; A01H; A01H4/00; C; C07; C07K; C07K14/00; C07K14/415; C07K14/425; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8201; C12N15/8202; C12N15/8205
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
49 words
A method for the somatic embryogenesis of a plant of a Glycine species comprising: (a) excising cotyledon tissue from immature embryos of said plant; (b) culturing said cotyledon tissue on an induction medium comprising an auxin of the NAA family at a concentration of at least about 15 mg/l.
Claim 2
Independent Claim
49 words
A method for the somatic embryogenesis of a plant of a Glycine species comprising: (a) excising cotyledon tissue from immature embryos of said plant; (b) culturing said cotyledon tissue on an induction medium comprising an auxin of the NAA family at a concentration of at least about 15 mg/l.
Claim 3
Dependent Claim
11 words
The method of claim 1 in which the auxin comprises NAA.
Claim 4
Dependent Claim
20 words
The method of claim 1 in which an embryo of step (b) is further cultured to regenerate a whole plant.
Claim 5
Dependent Claim
28 words
The method of claim 1 wherein said cotyledon tissue is transformed by the incorporation of foreign DNA into the genome thereof prior to the generation of somatic embryos.
Claim 6
Independent Claim
60 words
A method for regenerating a whole plant from somatic cells of a plant of a Glycine species comprising culturing said cells on a medium containing less than about 2% (w/v) carbohydrate and auxin at a concentration between about 5.0 mg/l and about 50 mg/l to generate an embryo and placing said embryo on appropriate media to generate a whole plant.
Claim 7
Independent Claim
60 words
A method for regenerating a whole plant from somatic cells of a plant of a Glycine species comprising culturing said cells on a medium containing less than about 2% (w/v) carbohydrate and auxin at a concentration between about 5.0 mg/l and about 50 mg/l to generate an embryo and placing said embryo on appropriate media to generate a whole plant.
Claim 8
Dependent Claim
23 words
The method of claim 5 wherein said somatic cells are taken from the fertile crescent region of cotyledon tissue taken from immature embryos.
Claim 9
Dependent Claim
27 words
The method of claim 6 wherein said cotyledon tissue is macerated by pressing against a mesh so as to separate said tissue into visible groups of cells.
Claim 10
Dependent Claim
25 words
The method of claim 6 or claim 7 wherein said somatic cells have been transformed by the insertion of foreign DNA into the genome thereof.
Claim 11
Independent Claim
84 words
A method of producing a plant of a Glycine species containing foreign DNA comprising: a) macerating cotyledon tissue taken from immature embryos of said species comprising the "fertile crescent" region; b) contacting said macerated tissue with said foreign DNA such that said DNA is incorporated into the cells of said tissue; c) contacting said macerated tissue with an embryogenic medium for said tissue to produce somatic embryos; d) transferring said somatic embryos to suitable media for regeneration of whole plants containing foreign DNA therefrom.
Claim 12
Independent Claim
84 words
A method of producing a plant of a Glycine species containing foreign DNA comprising: a) macerating cotyledon tissue taken from immature embryos of said species comprising the "fertile crescent" region; b) contacting said macerated tissue with said foreign DNA such that said DNA is incorporated into the cells of said tissue; c) contacting said macerated tissue with an embryogenic medium for said tissue to produce somatic embryos; d) transferring said somatic embryos to suitable media for regeneration of whole plants containing foreign DNA therefrom.
Claim 13
Dependent Claim
40 words
The method of claim 9 wherein following step (b) no selection medium which prevents or inhibits the growth of untransformed tissue is used, and transformants are identified and selected through the identification of a phenotype conferred by said foreign DNA.
References Cited (Backward Citations)
No citation records indexed.
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