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EP0256562A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Dry-type multilayer analytical element

Assignee: Fujifilm Holdings Corp
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Filing: 1987-08-19
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Granted: —
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Expires: 2007-08-19
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Abstract

Abstract In a dry-type multilayer analytical element containing a color reagent composition comprising an oxidase specifically reacting with an analyte or derived therefrom to produce hydrogen peroxide, hydrogen peroxide-decomposing enzyme, an alkali metal salt of ferrocyanide and a chromogen, this chromogen is separated from the hydrogen peroxide-decomposing enzyme, and the chromogen is incorporated in a layer nearer than the layer containing the hydrogen peroxide-decomposing enzyme to the transparent support of the analytical element. This analytical element is excellent in coloration, particularly stability of the color formed, and its result is accurate. Moreover, the results is hardly influenced by the presence of hemoglobin in a sample.

Inventors

  • 👤 Fuminori Arai
  • 👤 Asaji Kondo

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-08 14:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/90; C12Q2326/96; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/904

Claims Analysis

Total Claims: 3
Claim 1
Independent Claim 110 words
A dry-type multilayer analytical element having a layer containing a color forming reagent composition capable of determinating an analyte in an aqueous liquid sample by determinating hydrogen peroxide produced in the presence of oxidase on a light-transmissive water-impermeable support, said color forming reagent composition comprises an oxidase specifically reacting with said analyte or a material derived from said analyte to produce hydrogen peroxide, a hydrogen peroxide-decomposing enzyme, a ferrocyanide and a chromogen as essential components, the layer containing said chromogen is separated from the layer containing said hydrogen peroxide-decomposing enzyme, and said layer containing the chromogen is disposed nearer than said layer containing the hydrogen peroxide-decomposing enzyme to said support.
Claim 2
Dependent Claim 110 words
A dry-type multilayer analytical element having a layer containing a color forming reagent composition capable of determinating an analyte in an aqueous liquid sample by determinating hydrogen peroxide produced in the presence of oxidase on a light-transmissive water-impermeable support, said color forming reagent composition comprises an oxidase specifically reacting with said analyte or a material derived from said analyte to produce hydrogen peroxide, a hydrogen peroxide-decomposing enzyme, a ferrocyanide and a chromogen as essential components, the layer containing said chromogen is separated from the layer containing said hydrogen peroxide-decomposing enzyme, and said layer containing the chromogen is disposed nearer than said layer containing the hydrogen peroxide-decomposing enzyme to said support.
Claim 3
Dependent Claim 17 words
The dry-type multilayer analytical element of claim 1 wherein respective layers are integrally laminated on said support.

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