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EP0255377A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Acyl carrier protein- DNA sequence and synthesis

Assignee: Monsanto Co
•
Filing: 1987-07-30
•
Granted: —
•
Expires: 2007-07-30
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Abstract

Abstract DNA sequences are provided coding for acyl carrier protein, which sequence can be used for produc­tion of acyl carrier protein as an end product or in plant seed to enhance seed oil production. A regulated promoter is provided which substantially limits expres­sion of the acyl carrier protein to seed tissue.

Inventors

  • 👤 Jean C. Kridl
  • 👤 Vic C. Knauf

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-07 19:11
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/415; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8222; C12N15/823; C12N15/8234

Claims Analysis

Total Claims: 20
Claim 1
Independent Claim 9 words
A cDNA sequence coding for plant acyl carrier protein.
Claim 2
Dependent Claim 9 words
A cDNA sequence coding for plant acyl carrier protein.
Claim 3
Dependent Claim 12 words
A cDNA sequence according to Claim 1, wherein said plant is spinach.
Claim 4
Dependent Claim 13 words
A cDNA sequence according to Claim 1, wherein said plant is Brassica .
Claim 5
Dependent Claim 13 words
A cDNA sequence according to Claim 3, wherein said Brassica is campestris .
Claim 6
Dependent Claim 13 words
A cDNA sequence according to Claim 3, wherein said Brassica is napus .
Claim 7
Dependent Claim 51 words
A DNA construct comprising an open reading frame coding for a plant acyl carrier protein joined to and under transcriptional and translational initiation and termination regulatory regions function­al in a cellular host, wherein at least one of said regulatory regions is other than the wild-type region of said open reading frame.
Claim 8
Dependent Claim 51 words
A DNA construct comprising an open reading frame coding for a plant acyl carrier protein joined to and under transcriptional and translational initiation and termination regulatory regions function­al in a cellular host, wherein at least one of said regulatory regions is other than the wild-type region of said open reading frame.
Claim 9
Dependent Claim 20 words
A DNA construct according to Claim 6, wherein said open reading frame encodes for spinach or Brassica acyl carrier protein.
Claim 10
Dependent Claim 17 words
A DNA construct according to Claim 6, wherein said regulatory regions are functional in a plant host.
Claim 11
Dependent Claim 17 words
A DNA construct according to Claim 8, wherein said transcriptional initiation region is regu­lated during cell differentiation.
Claim 12
Dependent Claim 17 words
A DNA construct according to Claim 9, wherein said regulation provides for transcription in the developing embryo.
Claim 13
Dependent Claim 18 words
A DNA construct according to Claim 10, wherein said transcriptional initiation regulatory region is a napin regulatory region.
Claim 14
Dependent Claim 16 words
A Ti- or Ri-plasmid comprising a DNA construct according to any of Claims 6 to 11.
Claim 15
Dependent Claim 15 words
A plant cell comprising a DNA construct according to any of Claims 6 to 11.
Claim 16
Dependent Claim 20 words
A plant cell according to Claim 13, wherein said plant cell is an embryonic cell and part of a seed.
Claim 17
Dependent Claim 14 words
A plant cell according to Claim 14, wherein said plant is a Brassica plant.
Claim 18
Dependent Claim 44 words
A method for enhancing the formation of vegetable seed oils which comprises, growing a plant from plantlet to seed, wherein the cells of said plant comprise a construct according to Claim 8, and harvesting the resulting seed containing an enhanced amount of vegetable oil.
Claim 19
Dependent Claim 13 words
A method according to Claim 16, wherein said plant is a Brassica plant.
Claim 20
Independent Claim 20 words
A method according to any of Claims 16 or 17, wherein said transcriptional initiation regulatory region is a napin region.

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