EP0252750A1
Expired / Lapsed
EP
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Composition and analytical element having stabilized peroxidase
Assignee: Eastman Kodak Co
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Filing: 1987-07-10
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Granted: —
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Expires: 2007-07-10
Abstract
Abstract An analytical element has a peroxidase-labeled ligand analog distributed within a water-soluble binder composition comprising at least 50 percent, by weight, of poly(vinyl alcohol). As a result, the stability of peroxidase is improved prior to use. Such elements can be used to determine any of a number of immunologically reactive analytes, such as digoxin.
Inventors
- 👤 Jon Nathan Eikenberry
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 01:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
28 words
A water-soluble composition comprising a peroxidase-labeled ligand analog, the composition characterized as further comprising a water-soluble binder composition comprising at least 50 percent, by weight, of poly(vinyl alcohol).
Claim 2
Dependent Claim
28 words
A water-soluble composition comprising a peroxidase-labeled ligand analog, the composition characterized as further comprising a water-soluble binder composition comprising at least 50 percent, by weight, of poly(vinyl alcohol).
Claim 3
Dependent Claim
26 words
The composition as claimed in claim 1 wherein poly(vinyl alcohol) is present in the binder composition in an amount of at least 80 percent, by weight.
Claim 4
Dependent Claim
38 words
The composition as claimed in either of claims 1 or 2 wherein the binder composition comprises at least one additional binder material which is gelatin, polyacrylic acid, an acrylamide-N-vinyl-2-pyrrolidone copolymer, polyacrylamide, poly (N-vinyl-2-pyrrolidone) or a water-absorbent starch-containing polymer.
Claim 5
Dependent Claim
18 words
The composition as claimed in any of claims 1 to 3 wherein the ligand analog is peroxidase-labeled digoxin.
Claim 6
Dependent Claim
33 words
An analytical element for the determination of an immunologically reactive ligand comprising an absorbent carrier material, the element characterized as containing the water-soluble composition as claimed in any of claims 1 to 4.
Claim 7
Dependent Claim
34 words
The element as claimed in claim 5 further comprising an interactive composition which is capable of reacting with the ligand analog to provide a spectrophotometric signal in the presence of a substrate for peroxidase.
Claim 8
Dependent Claim
20 words
The element as claimed in claim 6 wherein the interactive composition comprises α-glycerol phosphate oxidase and a triarylimidazole leuco dye.
Claim 9
Dependent Claim
21 words
The element as claimed in any of claims 5 to 7 further comprising an immobilized receptor for the immunologically reactive ligand.
Claim 10
Independent Claim
86 words
A method for the determination of an immunologically reactive ligand comprising the steps of: A. in the presence of a receptor for the ligand, contacting a sample of a liquid suspected of containing the ligand with the analytical element as claimed in any of claims 5 to 8, the contacting carried out in such a manner as to form a complex of receptor and ligand analog, and B. determining the amount of the ligand as a result of the presence of complexed or uncomplexed ligand analog.
Claim 11
Dependent Claim
23 words
The method as claimed in claim 9 for the determination of digoxin in a biological fluid wherein the ligand analog is peroxidase-labeled digoxin.
References Cited (Backward Citations)
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