EP0252747A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Use of phenols and anilines to increase the rate of peroxidase catalyzed oxidation of leuco dyes
Assignee: Eastman Kodak Co
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Filing: 1987-07-10
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Granted: —
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Expires: 2007-07-10
Abstract
Abstract Certain phenols and anilines can be used as electron transfer agents to increase the rate of oxidation of leuco dyes by peroxidase. These phenols and anilines can react the hydrogen peroxide in the presence of peroxidase to provide intermediates which have higher oxidation potentials than the slowly oxidized substrates, for example, the leuco dyes. These phenols and anilines can be used to advantage in both solution and dry assays of various analytes. They are particularly useful for the determination of an immunologically reactive ligand in an immunoassay.
Inventors
- 👤 Gregory c/o Eastman Kodak Co. McClune
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 11:28
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/581
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
75 words
An analytical composition comprising peroxidase or a peroxidase-labeled analog of an immunologically reactive ligand and a leuco dye which is capable of providing a detectable dye in the presence of peroxidase and hydrogen peroxide, the composition characterized as further comprising a phenol or aniline electron transfer agent which is capable of reacting with hydrogen peroxide in the presence of peroxidase to provide an intermediate compound which has a higher oxidation potential than the leuco dye.
Claim 2
Dependent Claim
75 words
An analytical composition comprising peroxidase or a peroxidase-labeled analog of an immunologically reactive ligand and a leuco dye which is capable of providing a detectable dye in the presence of peroxidase and hydrogen peroxide, the composition characterized as further comprising a phenol or aniline electron transfer agent which is capable of reacting with hydrogen peroxide in the presence of peroxidase to provide an intermediate compound which has a higher oxidation potential than the leuco dye.
Claim 3
Dependent Claim
15 words
The composition as claimed in claim 1 wherein the leuco dye is an imidazole derivative.
Claim 4
Dependent Claim
31 words
The composition as claimed in either of claims 1 or 2 wherein the phenol or aniline electron transfer agent is p , p ʹ-biphenol, 4ʹ-hydroxyacetanilide, p -methoxyphenol, p -anisidine or 4ʹ-aminoacetanilide.
Claim 5
Dependent Claim
28 words
The composition as claimed in any of claims 1 to 3 further comprising an interactive composition which is capable of interacting with an analyte to provide hydrogen peroxide.
Claim 6
Dependent Claim
28 words
The composition as claimed in any of claims 1 to 4 comprising peroxidase and an analog of an immunologically reactive ligand labeled with an enzyme other than peroxidase.
Claim 7
Dependent Claim
14 words
The composition as claimed in claim 5 wherein the ligand analog comprises glucose oxidase.
Claim 8
Dependent Claim
25 words
An analytical element comprising an absorbent carrier material, the element characterized as containing the analytical composition as claimed in any of claims 1 to 6.
Claim 9
Dependent Claim
16 words
The element as claimed in claim 7 further comprising a receptor for an immunologically reactive ligand.
Claim 10
Independent Claim
54 words
A method for the determination of an analyte comprising the steps of: A. contacting a sample of a liquid suspected of containing an analyte with the analytical composition or element as claimed in any of claims 1 to 8, and B. determining the detectable dye as a result of the presence of the analyte.
Claim 11
Dependent Claim
40 words
The method as claimed in claim 9 for the determination of an analyte other than hydrogen peroxide wherein the liquid sample is also contacted with an interactive composition which is capable of reacting with the analyte to provide hydrogen peroxide.
Claim 12
Dependent Claim
26 words
The method as claimed in claim 9 for the determination of an immunologically reactive ligand carried out in the presence of a receptor for the ligand.
References Cited (Backward Citations)
No citation records indexed.
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