EP0246103A2
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EP
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Bioaffinity and ion exchange separations with liquid exchange supports
Assignee: EIDP Inc
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Filing: 1987-05-14
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Granted: —
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Expires: 2007-05-14
Abstract
Abstract Bioaffinity and ion-exchange separation methods are provided along with liquid supports utilized in these methods. The support is based on an inert carrier with ligands or binders attached to its surface. Methods for preparing such supports and their use in capturing neutral and charged target molecules from samples and in analytical applications are also provided.
Inventors
- 👤 Julian P. Breillatt
- 👤 John William Eveleigh
- 👤 Norman Alfred Parris
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 01:40
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/551
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
48 words
A liquid support consisting essentially of: (A) a chemically inert, water immiscible liquid carrier having specific gravity significantly different from the specific gravity of water and further having low nonspecific binding to ligands or binders; and (B) ligands or binders securely attached to the surface of said carrier.
Claim 2
Dependent Claim
48 words
A liquid support consisting essentially of: (A) a chemically inert, water immiscible liquid carrier having specific gravity significantly different from the specific gravity of water and further having low nonspecific binding to ligands or binders; and (B) ligands or binders securely attached to the surface of said carrier.
Claim 3
Dependent Claim
13 words
A liquid support according to claim 1 which is a liquid affinity support.
Claim 4
Dependent Claim
20 words
A liquid affinity support according to claim 2 wherein the liquid carier is selected from liquid perfluorocarbon, hydrocarbon and silicone.
Claim 5
Dependent Claim
29 words
A liquid affinity support according to claim 2 or 3, wherein said ligand is selected from antigen, hapten, nucleic acid, enzyme substrate, vitamin dye and enzyme effectors and inhibitors.
Claim 6
Dependent Claim
28 words
A liquid affinity support according to claim 2 or 3, wherein said binder is selected from antibody, enzyme, nucleic acid, binding protein and synthetic mimics of binding proteins.
Claim 7
Dependent Claim
59 words
A bioaffinity separation process comprising the steps of: (A) forming a liquid affinity support by attaching a ligand or binder to the surface of the droplets of an emulsion of a liquid carrier; and (B) capturing a target binder or ligand, complementary to the ligand or binder attached to the carrier from a mixture using said liquid affinity support.
Claim 8
Dependent Claim
59 words
A bioaffinity separation process comprising the steps of: (A) forming a liquid affinity support by attaching a ligand or binder to the surface of the droplets of an emulsion of a liquid carrier; and (B) capturing a target binder or ligand, complementary to the ligand or binder attached to the carrier from a mixture using said liquid affinity support.
Claim 9
Dependent Claim
22 words
A process according to claim 6 wherein the liquid affinity support is as claimed in any one of claims 2 to 5.
Claim 10
Dependent Claim
36 words
A process for preparing a liquid affinity support comprising the steps of: (A) dissolving a reactive perfluorinated compound in a liquid perfluorocarbon; and (B) emulsifying said solution with an aqueous solution of a ligand or binder.
Claim 11
Dependent Claim
36 words
A process for preparing a liquid affinity support comprising the steps of: (A) dissolving a reactive perfluorinated compound in a liquid perfluorocarbon; and (B) emulsifying said solution with an aqueous solution of a ligand or binder.
Claim 12
Dependent Claim
39 words
A process for preparing a liquid affinity support comprising the steps of: (A) modifying a ligand or binder by attaching a highly fluorinated anchor group thereto; and (B) emulsifying a liquid perfluorocarbon with the anchor modified ligand or binder.
Claim 13
Dependent Claim
39 words
A process for preparing a liquid affinity support comprising the steps of: (A) modifying a ligand or binder by attaching a highly fluorinated anchor group thereto; and (B) emulsifying a liquid perfluorocarbon with the anchor modified ligand or binder.
Claim 14
Dependent Claim
21 words
A process according to claim 8 or 9 wherein the ligand or buffer is as defined in claim 4 or 5.
Claim 15
Dependent Claim
69 words
An assay for a nucleic acid comprising the steps of: (A) capturing a nucleic acid from a sample suspected of containing the nucleic acid by contacting said sample with a liquid affinity support having a complementary binder on its surface; (B) concentrating the support:nucleic acid complex formed in step (A) to form a binder:nucleic acid complex: and (C) analyzing for the nucleic acid by a nucleic acid hybridization assay.
Claim 16
Dependent Claim
69 words
An assay for a nucleic acid comprising the steps of: (A) capturing a nucleic acid from a sample suspected of containing the nucleic acid by contacting said sample with a liquid affinity support having a complementary binder on its surface; (B) concentrating the support:nucleic acid complex formed in step (A) to form a binder:nucleic acid complex: and (C) analyzing for the nucleic acid by a nucleic acid hybridization assay.
Claim 17
Dependent Claim
22 words
The assay according to claim 11, wherein the binder is selected from binding protein, nucleic acid and synthetic mimics of binding proteins.
Claim 18
Dependent Claim
70 words
An immunoassay for detecting an analyte comprising the steps of: (A) capturing an analyte from a sample suspected of containing the analyte by contacting said sample with a liquid affinity support having the member of the bioaffinity binding pair complementary to said analyte on its surface; (B) concentrating the support:analyte complex formed in step (A) to form a binder:analyte complex; and (C) analyzing for the analyte by an appropriate immunoassay.
Claim 19
Dependent Claim
70 words
An immunoassay for detecting an analyte comprising the steps of: (A) capturing an analyte from a sample suspected of containing the analyte by contacting said sample with a liquid affinity support having the member of the bioaffinity binding pair complementary to said analyte on its surface; (B) concentrating the support:analyte complex formed in step (A) to form a binder:analyte complex; and (C) analyzing for the analyte by an appropriate immunoassay.
Claim 20
Dependent Claim
13 words
A liquid support according to claim 1 which is a liquid ion-exchange support.
Claim 21
Dependent Claim
57 words
A liquid ion-exchange support according to claim 14 consisting essentially of: (A) a chemically inert, water immiscible liquid carrier having specific gravity significantly different from the specific gravity of water and further having low nonspecific binding to ligands or binders; and (B) a reagent comprised of a highly fluorinated or perfluoro-substituted moiety and a charged organic moiety.
Claim 22
Dependent Claim
14 words
A support according to claim 15 wherein said reagent (B) is a charged fluoro-surfactant.
Claim 23
Dependent Claim
57 words
An ion-exchange separation process comprising the steps of: (A) forming a ligand ion-exchange support by attaching a charged organic moiety to the surface of the droplets of an emulsion of a liquid carrier; and (B) capturing a target molecule having a charge opposite to that attached to the carrier from a mixture using said liquid ion-exchange support.
Claim 24
Dependent Claim
57 words
An ion-exchange separation process comprising the steps of: (A) forming a ligand ion-exchange support by attaching a charged organic moiety to the surface of the droplets of an emulsion of a liquid carrier; and (B) capturing a target molecule having a charge opposite to that attached to the carrier from a mixture using said liquid ion-exchange support.
References Cited (Backward Citations)
No citation records indexed.
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