Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
50 words
A method of quantitatively measuring hydrogen peroxide in a sample, which comprises the steps of adding to the sample an aromatic compound with a hydroxy radical, a diamine compound and an oxidase to cause a reaction and form a quinonic dye, and determining the color tone (intensity) of the dye.
Claim 2
Independent Claim
50 words
A method of quantitatively measuring hydrogen peroxide in a sample, which comprises the steps of adding to the sample an aromatic compound with a hydroxy radical, a diamine compound and an oxidase to cause a reaction and form a quinonic dye, and determining the color tone (intensity) of the dye.
Claim 3
Dependent Claim
23 words
A method as claimed in Claim 1, wherein said hydroxy radical containing aromatic compound is selected from the group of phenols and naphthols.
Claim 4
Dependent Claim
67 words
A method as claimed in Claim 1 or 2, wherein said diamine compound is an aniline compound of the formula wherein Z 1 and Z 2 are alkyl group, acyl group or sulfo radical, respectively, or Z 1 is a heterocyclic- group together with Z 2 and the neighbouring nitrogen atom, Z 3 and Z 4 are hydrogen atom, halogen atom, alkyl group or acyl group, respectively.
Claim 5
Dependent Claim
15 words
A method as claimed in Claim 1, 2 or 3, wherein said oxidase is peroxidase.
Claim 6
Dependent Claim
33 words
A method as claimed in Claim 2, wherein said phenol compound is that of the formula wherein X 1 and X 2 are hydrogen atom, halogen atom, alkyl group or nitro radical, respectively.
Claim 7
Dependent Claim
35 words
A method as claimed in Claim 2, wherein said naphthol compound is that of the formula wherein Y 1 and Y 2 are hydrogen atom, halogen atom, nitro radical, sulfo radical or acyl group, respectivcely.
Claim 8
Dependent Claim
13 words
A method as claimed in Claim 3, wherein said aniline compound is p-aminodiethylaniline.
Claim 9
Dependent Claim
84 words
8, A method of quantitatively measuring biochemical substances and enzymes in a sample, which comprises steps of reacting the biochemical substance with the enzyme to liberate hydrogen peroxide, adding an aromatic compound with a hydroxy radical, a diamine compound and an oxidase as claimed in any preceding claim, to cause a reaction with the hydrogen peroxide and form a quinonic dyes and determine the color tone of the dye so as to measure the amount of the biochemical substance or enzyme in the sample:.