EP0243001A1
Expired / Lapsed
EP
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Liquid single reagent for assays
Assignee: Syntex USA LLC
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Filing: 1987-03-24
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Granted: —
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Expires: 2007-03-24
Abstract
Abstract Assay methods and compositions are provided for determining an analyte in a sample suspected of containing the analyte. The composition comprises in a novel single liquid reagent at least one specific binding pair (sbp) member and its complementary member wherein at least one sbp member is reversibly confined in a material that temporarily renders the confined sbp member incapable of binding with its complementary sbp member. At least one of the sbp members is bound to a member of a signal producing system capable of producing a detectable signal in relation to the amount of analyte in the sample. The confinement is reversed, any remaining members of the signal producing system are added, and the signal produced in relation to the amount of analyte is measured.
Inventors
- 👤 Ian Gibbons
- 👤 Philip L. Felgner
- 👤 Edwin F. Ullman
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 22:54
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/549; G01N33/54313
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
160 words
An assay method for determining an analyte in a sample suspected of containing the analyte wherein the analyte is a member of a specific binding pair (sbp) consisting of ligand and its complementary receptor and wherein a signal producing system is employed which provides a detectable signal in relation to the amount of analyte in said sample, said assay comprising the steps of: a) combining a sample suspected of containing an analyte with a composition comprising in a single liquid medium (1) at least one sbp member reversibly confined in a material rendering said sbp member temporarily incapable of binding with its complementary sbp member, and (2) said complementary sbp member wherein at least one of said sbp members is bound to a member of a signal producing system; and b) providing means for reversing the confinement of said sbp member from said material; and c) measuring a signal produced in relation to the amount of analyte in said sample.
Claim 2
Dependent Claim
160 words
An assay method for determining an analyte in a sample suspected of containing the analyte wherein the analyte is a member of a specific binding pair (sbp) consisting of ligand and its complementary receptor and wherein a signal producing system is employed which provides a detectable signal in relation to the amount of analyte in said sample, said assay comprising the steps of: a) combining a sample suspected of containing an analyte with a composition comprising in a single liquid medium (1) at least one sbp member reversibly confined in a material rendering said sbp member temporarily incapable of binding with its complementary sbp member, and (2) said complementary sbp member wherein at least one of said sbp members is bound to a member of a signal producing system; and b) providing means for reversing the confinement of said sbp member from said material; and c) measuring a signal produced in relation to the amount of analyte in said sample.
Claim 3
Dependent Claim
32 words
The method according to claim 1 wherein the member of said signal producing system is selected from enzymes, co-enzymes, fluorescers, dyes, substrates, chemiluminescers, and co-factors, preferably wherein said enzyme is glucose-6-phosphate dehydrogenase.
Claim 4
Dependent Claim
18 words
The method according to claim 1 or claim 2 wherein said analyte is selected from antigens and antibodies.
Claim 5
Independent Claim
34 words
The method according to any one of the preceding claims wherein said analyte is selected from drugs, proteins, polypeptides, nucleic acids and polysaccharides, preferably wherein said drugs are selected from theophylline, thyroxine and digoxin.
Claim 6
Independent Claim
57 words
The method according to any one of the preceding claims wherein said means for reversing the confinement of said sbp member is a chemical means selected from detergents, polypeptides, organic solvents, and complement; or a physical means selected from a change in temperature, sonication and osmotic shock; or a combination of a chemical and a physical means.
Claim 7
Independent Claim
30 words
The method according to any one of the preceding claims wherein said material is a naturally occurring material, preferably red blood cell ghosts, or a synthetic material, preferably a liposome.
Claim 8
Dependent Claim
55 words
The method according to claim 1 wherein said reversibly confined member is a conjugate of an enzyme and a hapten and said complementary member is antibody for said hapten and said material is a liposome, preferably wherein said reversibly confined member is glucose-6-phosphate dehydrogenase-theophylline said non-confined member is anti-theophylline and said material is a liposome.
Claim 9
Dependent Claim
24 words
The method of claim 6 or claim 7 wherein the material is a liposome and said liposome comprises palmitoyl oleoylphosphatidylcholine, palmitoyl phosphatidylglycerol and cholesterol.
Claim 10
Dependent Claim
81 words
A composition for conducting an assay for an analyte which is a member of a specific binding pair (sbp) consisting of ligand and its complementary receptor, said composition comprising in a liquid medium: (a) at least one sbp member reversibly confined in a material rendering said sbp member temporarily incapable of binding with its complementary sbp member; and (b) said complementary sbp member; wherein at least one of said sbp members is bound to a member of a signal producing system.
Claim 11
Dependent Claim
81 words
A composition for conducting an assay for an analyte which is a member of a specific binding pair (sbp) consisting of ligand and its complementary receptor, said composition comprising in a liquid medium: (a) at least one sbp member reversibly confined in a material rendering said sbp member temporarily incapable of binding with its complementary sbp member; and (b) said complementary sbp member; wherein at least one of said sbp members is bound to a member of a signal producing system.
Claim 12
Dependent Claim
18 words
A composition comprising a solid obtained by evaporative removal of the liquid from the composition of claim 9.
Claim 13
Dependent Claim
18 words
The composition according to claim 9 or claim 10 wherein said analyte is selected from antibodies and antigens.
Claim 14
Dependent Claim
37 words
The composition according to any one of claims 9 to 11 wherein said member of said signal producing system is selected from enzymes, co-enzymes, fluorescers, dyes, substrates, chemiluminescers, and co-factors, preferably wherein said enzyme is glucose-6-phosphate dehydrogenase.
Claim 15
Dependent Claim
31 words
The composition according to any one of claims 9 to 12 wherein said material is a naturally occurring material, preferably red blood cell ghosts, or a synthetic material, preferably a liposome.
Claim 16
Dependent Claim
55 words
The composition according to claim 9 wherein said reversibly confined member is a conjugate of an enzyme and a hapten and said complementary member is antibody for said hapten and said material is a liposome, preferably wherein said reversibly confined member is glucose-6-phosphate dehydrogenase-theophylline, said complementary member is anti-theophylline, and said material is a liposome.
Claim 17
Dependent Claim
24 words
The composition of claim 13 or claim 14 wherein said material is a liposome and said liposome comprises palmitoyl oleoylphosphatidylcholine, palmitoyl phosphatidylglycerol and cholesterol.
Claim 18
Dependent Claim
47 words
A kit for use in an assay for determining an analyte in a sample suspected of containing the analyte comprising in combination: a) a composition according to any one of claims 9 to 15; and b) a reagent for reversing the confinement of the confined sbp member.
Claim 19
Dependent Claim
123 words
An assay for determining an analyte in a sample suspected of containing the analyte wherein the analyte is a member of a specific binding pair (sbp) consisting of ligand and its complementary receptor and wherein a signal producing system is employed which provides a detectable signal in relation to the amount of analyte in the sample, characterised in that there is employed as a reagent in said assay a composition comprising in a single liquid medium (1) at least one sbp member reversibly confined in a material rendering said sbp member temporarily incapable of binding with its complementary sbp member and (2) said complementary sbp member, wherein at least one of said sbp members is bound to member of a signal producing system.
Claim 20
Dependent Claim
123 words
An assay for determining an analyte in a sample suspected of containing the analyte wherein the analyte is a member of a specific binding pair (sbp) consisting of ligand and its complementary receptor and wherein a signal producing system is employed which provides a detectable signal in relation to the amount of analyte in the sample, characterised in that there is employed as a reagent in said assay a composition comprising in a single liquid medium (1) at least one sbp member reversibly confined in a material rendering said sbp member temporarily incapable of binding with its complementary sbp member and (2) said complementary sbp member, wherein at least one of said sbp members is bound to member of a signal producing system.
References Cited (Backward Citations)
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