EP0242847A2
Expired / Lapsed
EP
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Tracers for use in flecainide fluorescence polarization immunoassay
Assignee: Abbott Laboratories
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Filing: 1987-04-21
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Granted: —
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Expires: 2007-04-21
Abstract
Abstract The present invention is directed to a fluorescence polarization assay for determining the amount of flecainide in fluids, especially biological fluids such as serum, plasma or urine. The present invention also relates to novel tracer compounds useful in fluorescence polarization assay techniques.
Inventors
- 👤 Daniel Feulner Heiman
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 18:19
CPC Classifications
C; C07; C07D; C07D493/00; C07D493/02; C07D493/10; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/94; G01N33/9453
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
59 words
A compound comprising the structure: wherein: Z is a linking group including up to 10 heteroatoms, and having a total of from 0 to 20 carbon atoms and heteroatoms arranged in a straight or branched chain and containing up to two ring structures; A is NH, C=0, SO 2 or C=NH; and Q is fluorescein or a fluorescein derivative.
Claim 2
Dependent Claim
59 words
A compound comprising the structure: wherein: Z is a linking group including up to 10 heteroatoms, and having a total of from 0 to 20 carbon atoms and heteroatoms arranged in a straight or branched chain and containing up to two ring structures; A is NH, C=0, SO 2 or C=NH; and Q is fluorescein or a fluorescein derivative.
Claim 3
Dependent Claim
9 words
The compound of Claim 1 wherein Q is (fluorescein-5-yl)amino.
Claim 4
Dependent Claim
9 words
The compound of Claim 1 wherein Q is (fluorescein-6-yl)amino.
Claim 5
Dependent Claim
9 words
The compound of Claim 1 wherein Q is (fluorescein-5-yl)carbonyl.
Claim 6
Dependent Claim
9 words
The compound of Claim 1 wherein Q is (fluorescein-6-yl)carbonyl.
Claim 7
Dependent Claim
11 words
The compound of Claim 1 wherein -R-Z-Q has the following structure:
Claim 8
Dependent Claim
11 words
The compound of Claim 1 wherein -R-Z-Q has the following structure:
Claim 9
Independent Claim
80 words
A method for making a tracer comprising the step of coupling a precursor of the formula wherein: R is a linking group including up to 10 heteroatoms and having a total of from 0 to 20 carbon atoms and heteroatoms arranged in a straight or branched chain and containing up to two ring structures; Z is NH, S, 0, C=O, SO 2 or C=NH; and Q is hydrogen, hydroxyl or a leaving group; with fluorescein or a derivative of fluorescein.
Claim 10
Independent Claim
80 words
A method for making a tracer comprising the step of coupling a precursor of the formula wherein: R is a linking group including up to 10 heteroatoms and having a total of from 0 to 20 carbon atoms and heteroatoms arranged in a straight or branched chain and containing up to two ring structures; Z is NH, S, 0, C=O, SO 2 or C=NH; and Q is hydrogen, hydroxyl or a leaving group; with fluorescein or a derivative of fluorescein.
Claim 11
Dependent Claim
88 words
A process for measuring the concentration of flecainide in an aqueous solution which comprises the steps of: a) contacting a sample with a flecainide antiserum and with a compound according to Claim 1 capable of producing detectable fluorescence polarization response to the presence of flecainide antiserum; b) passing plane polarized light through the solution resulting from step a) to obtain a fluorescence polarization response; and c) detecting the fluorescence polarization response of the solution of step b) as a measure of the amount of flecainide in the sample.
References Cited (Backward Citations)
No citation records indexed.
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