EP0240348A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Agent for the removal of nucleic acids and/or endotoxin and method for the removal thereof
Assignee: Kurita Water Industries Ltd
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Filing: 1987-04-02
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Granted: —
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Expires: 2007-04-02
Abstract
Abstract An agent for the removal of nucleic acids and/or endotoxin from a liquid containing the nucleic acids and/or endotoxin and further useful substances (e.g. proteins, hormones, etc.) comprises as an active ingredient a chitosan having a low molecular weight, particularly that having an intricsin viscosity of 0.01 to 5 (dl/g) and a colloid equivalent of not less than 2 meq/g of evaporated residue.
Inventors
- 👤 Masanori Hashimoto
- 👤 Takeshi Yamamoto
- 👤 Toru Kawachi
- 👤 Junji Kuwashima
- 👤 Hirokazu Kitaoka
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 08:50
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K1/00; C07K1/14; C07K14/00; C07K14/435; C07K14/52; C07K14/525; C07K14/54; C07K14/545
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
23 words
An agent for the removal of nucleic acids and/or endotoxin which comprises as an active ingredient a chitosan having a low molecular weight.
Claim 2
Dependent Claim
23 words
An agent for the removal of nucleic acids and/or endotoxin which comprises as an active ingredient a chitosan having a low molecular weight.
Claim 3
Dependent Claim
23 words
The agent according to claim 1, wherein the chitosan having a low molecular weight has an intrinsic viscosity of 0.01 to 5 (dl/g).
Claim 4
Dependent Claim
30 words
The agent according to claim 1, wherein the chitosan having a low molecular weight has a colloid equivalent of not lower than 2 meq/g of evaporated residue at pH 4.
Claim 5
Dependent Claim
39 words
The agent according to claim 1, wherein the chitosan having a low molecular weight has an intrinsic viscosity of 0.2 to 1 (dl/g) and a colloid equivalent of not less than 4 meq/g of evaporated residue at pH 4.
Claim 6
Dependent Claim
30 words
The agent according to claim 1, which is useful for the separation of nucleic acids and/or endotoxin from a liquid to be treated which contains the nucleic acid and/or endotoxin.
Claim 7
Dependent Claim
24 words
The agent according to claim 5, wherein the liquid to be treated contains further proteins having an isoelectric point of not less than 3.
Claim 8
Dependent Claim
14 words
The agent according to claim 6, wherein the protein is human tumor necrosis factor.
Claim 9
Dependent Claim
13 words
The agent according to claim 6, wherein the protein is human interleukin 1.
Claim 10
Dependent Claim
16 words
The agent according to claim 5, wherein the liquid to be treated is a cell extract.
Claim 11
Independent Claim
32 words
A method for the removal of nucleic acids and/or endotoxin from a liquid containing the nucleic acids and/or endotoxin, which comprises treating the liquid with a chitosan having a low molecular weight.
Claim 12
Independent Claim
32 words
A method for the removal of nucleic acids and/or endotoxin from a liquid containing the nucleic acids and/or endotoxin, which comprises treating the liquid with a chitosan having a low molecular weight.
Claim 13
Dependent Claim
23 words
The method according to claim 10, wherein the chitosan having a low molecular weight has an intrinsic viscosity of 0.01 to 5 (dl/g).
Claim 14
Dependent Claim
30 words
The method according to claim 10, wherein the chitosan having a low molecular weight has a colloid equivalent of not lower then 2 meq/g of evaporated residue at pH 4.
Claim 15
Dependent Claim
39 words
The method according to claim 10, wherein the chitosan having a low molecular weight has an intrinsic viscosity of 0.2 to 1 (dl/g) and a colloid equivalent of not less than 4 meq/g of evaporated residue at pH 4.
Claim 16
Dependent Claim
29 words
The method according to claim 10, which is for the separation of nucleic acids and/or endotoxin from a liquid to be treated which contains the nucleic acids and/or endotoxin.
Claim 17
Dependent Claim
24 words
The method according to claim 14, wherein the liquid to be treated contains further proteins having an isoelectric point of not less than 3.
Claim 18
Dependent Claim
14 words
The method according to claim 15, wherein the protein is human tumor necrosis factor.
Claim 19
Dependent Claim
13 words
The method according to claim 15, wherein the protein is human interleukin 1.
Claim 20
Dependent Claim
16 words
The method according to claim 11, wherein the liquid to be treated is a cell extract.
Claim 21
Dependent Claim
31 words
A composition for the removal of at least one of nucleic acids and endotoxin, which composition comprises a chitosan having a low molecular weight and a diluent, carrier or precipitating aid.
Claim 22
Dependent Claim
31 words
A composition for the removal of at least one of nucleic acids and endotoxin, which composition comprises a chitosan having a low molecular weight and a diluent, carrier or precipitating aid.
References Cited (Backward Citations)
No citation records indexed.
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