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EP0240191A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Method of isolating DNA contained in a virus or cell

Assignee: Seiko Instruments Inc
•
Filing: 1987-03-12
•
Granted: —
•
Expires: 2007-03-12
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Abstract

Abstract A method of isolating DNA contained in a virus or a cell comprises the steps of; isolating the virus or cell having satellite DNA ; reacting a protein decomposer with said virus or cell for destroying or denaturing protein covering DNA; adding at least twice by volume of alcohol to the reaction mixture for precipitating DNA; feeding an alcohol containing solution obtained in the previous step into a separation column for retaining the precipitated DNA; passing an aqueous alcohol solution having a high alcohol concentration through the column for removing impurity; and passing an aqueous solution having a low alcohol concentration or an aqueous solution free from alcohol through said column for eluating said DNA.

Inventors

  • 👤 Murata Masafumi
  • 👤 Sakaba Munechika

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-05 19:44
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12N15/1017

Claims Analysis

Total Claims: 5
Claim 1
Independent Claim 117 words
A method of isolating DNA contained in a virus or a cell characterised by comprising the steps of: isolating the virus or cell having satellite DNA; reacting a protein decomposer with said virus or cell for destroying or denaturing protein covering DNA; adding at least twice by volume of alcohol to the reaction mixture to precipitate the DNA; feeding an alcohol containing solution obtained in the previous step into a separation column for retaining the precipitated DNA; passing an aqueous alcohol solution having a high alcohol concentration through said column to remove impurity; and passing an aqueous solution having a low alcohol concentration or an aqueous solution free from alcohol through said column for eluating said DNA.
Claim 2
Independent Claim 117 words
A method of isolating DNA contained in a virus or a cell characterised by comprising the steps of: isolating the virus or cell having satellite DNA; reacting a protein decomposer with said virus or cell for destroying or denaturing protein covering DNA; adding at least twice by volume of alcohol to the reaction mixture to precipitate the DNA; feeding an alcohol containing solution obtained in the previous step into a separation column for retaining the precipitated DNA; passing an aqueous alcohol solution having a high alcohol concentration through said column to remove impurity; and passing an aqueous solution having a low alcohol concentration or an aqueous solution free from alcohol through said column for eluating said DNA.
Claim 3
Dependent Claim 72 words
A method as claimed in claim 1 characterised by comprising the steps of adding a virus particle aggregating agent to a double suspension comprising a virus and a host cell therefor, subjecting the mixture containing an aggregated virus and host cell obtained in the previous step to filtration with a filter to filter off said aggregated virus and host cell, and eluating said virus with water or a buffer from said filter.
Claim 4
Dependent Claim 17 words
A method as claimed in claim 2 characterised in that the virus particle-aggregating agent is polyethylene glycol.
Claim 5
Dependent Claim 18 words
A method as claimed in claim 1 characterised in that isolation of a cell is conducted by filtration.

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