EP0239400A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Recombinant antibodies and methods for their production
Assignee: Medical Research Council
•
Filing: 1987-03-26
•
Granted: —
•
Expires: 2007-03-26
Abstract
Abstract An altered antibody is produced by replacing the complementarity determining regions (CDRs) of a variable region of an immunoglobulin (Ig) with the CDRs from an Ig of different specificity, using recombinant DNA techniques. The gene coding sequences for producing the altered antibody may be produced by site-directed mutagenesis using long oligonucleotides.
Inventors
- 👤 Gregory Paul Winter
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 04:52
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/46; C07K16/461; C07K16/464; C07K2317/00; C07K2317/20; C07K2317/24; C07K2319/00
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
36 words
An altered antibody in which at least parts of the complementarity determining regions (CDRs) in the light or heavy chain variable domains have been replaced by analogous parts of CDRs from an antibody of different specificity.
Claim 2
Dependent Claim
36 words
An altered antibody in which at least parts of the complementarity determining regions (CDRs) in the light or heavy chain variable domains have been replaced by analogous parts of CDRs from an antibody of different specificity.
Claim 3
Dependent Claim
16 words
The altered antibody of claim 1, in which the entire CD R s have been replaced.
Claim 4
Dependent Claim
27 words
The altered antibody of claim 1 or claim 2, in which the variable domains in both the heavy and light chains have been altered by CDR replacement.
Claim 5
Dependent Claim
29 words
The altered antibody of any one of claims 1 to 3 in which the CDRs from a mouse antibody are grafted onto the framework regions of a human antibody.
Claim 6
Dependent Claim
23 words
The altered antibody of any one of claims 1 to 4, which has the structure of a natural antibody or a fragment thereof.
Claim 7
Independent Claim
126 words
A method for producing an altered antibody comprising: a) preparing a first replicable expression vector including a suitable promoter operably linked to a DNA sequence which encodes at least a variable domain of an Ig heavy or light chain, the variable domain comprising framework regions from a first antibody and C DR s comprising at least parts of the CDRs from a second antibody of different specificity; b) if necessary, preparing a second replicable expression vector including a suitable promoter operably linked to a DNA sequence which encodes at least the variable domain of a complementary Ig light or heavy chain respectively; c) transforming a cell line with the first or both prepared vectors; and d) culturing said transformed cell line to produce said altered antibody.
Claim 8
Independent Claim
126 words
A method for producing an altered antibody comprising: a) preparing a first replicable expression vector including a suitable promoter operably linked to a DNA sequence which encodes at least a variable domain of an Ig heavy or light chain, the variable domain comprising framework regions from a first antibody and C DR s comprising at least parts of the CDRs from a second antibody of different specificity; b) if necessary, preparing a second replicable expression vector including a suitable promoter operably linked to a DNA sequence which encodes at least the variable domain of a complementary Ig light or heavy chain respectively; c) transforming a cell line with the first or both prepared vectors; and d) culturing said transformed cell line to produce said altered antibody.
Claim 9
Dependent Claim
24 words
The method of claim 6, in which the cell line which is transformed to produce the altered antibody is an immortalised mammalian cell line.
Claim 10
Dependent Claim
20 words
The method of claim 7, in which the immortalised cell line is a myeloma cell line or a derivative thereof.
Claim 11
Independent Claim
25 words
The method of any one of claims 6 to 8, in which the DNA sequence encoding the altered variable domain is prepared by oligonucleotide synthesis.
Claim 12
Independent Claim
32 words
The method of any one of claims 6 to 8, in which the DNA sequence encoding the altered variable domain is prepared by primer directed oligonucleotide site-directed mutagenesis using a long oligonucleotide.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.