EP0238323A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Improvements in or relating to tylosin antibiotic-producing microorganisms
Assignee: Eli Lilly and Co
•
Filing: 1987-03-18
•
Granted: —
•
Expires: 2007-03-18
Abstract
Abstract A means for increasing the antibiotic-producing ability of an antibiotic-producing microbial host cell is disclosed. The method involves transforming an antibiotic-producing microorganism with a recombinant DNA cloning vector that codes for the expression of a rate-limiting antibiotic biosynthetic enzyme or other gene product.
Inventors
- 👤 Karen Leigh Cox
- 👤 Scott Eric Fishman
- 👤 Charles Lee Hershberger
- 👤 Eugene Thomas Seno
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 04:31
CPC Classifications
C; C12; C12N; C12N1/00; C12N9/00; C12N9/10; C12N9/1003; C12N9/1007; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N15/63; C12N15/70; C12P; C12P19/00; C12P19/44; C12P19/60; C12P19/62
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
101 words
A method for increasing the antibiotic- or antibiotic precursor-producing ability of an antibiotic-producing microorganism, which comprises culturing a microorganism which produces an antibiotic or antibiotic precursor by a biosynthetic pathway, said microorganism being transformed with a DNA cloning vector or portion thereof which contains an antibiotic or antibiotic-precursor biosynethetic gene coding for expression of a rate-limiting enzyme or gene product of the biosynthetic pathway, under conditions suitable for cell growth, expression of the antibiotic or antibiotic-precursor biosynthetic gene and production of the antibiotic or antibiotic precursor, provided that the culturing process provides an increase in the antibiotic-producing ability of the microorganism.
Claim 2
Independent Claim
101 words
A method for increasing the antibiotic- or antibiotic precursor-producing ability of an antibiotic-producing microorganism, which comprises culturing a microorganism which produces an antibiotic or antibiotic precursor by a biosynthetic pathway, said microorganism being transformed with a DNA cloning vector or portion thereof which contains an antibiotic or antibiotic-precursor biosynethetic gene coding for expression of a rate-limiting enzyme or gene product of the biosynthetic pathway, under conditions suitable for cell growth, expression of the antibiotic or antibiotic-precursor biosynthetic gene and production of the antibiotic or antibiotic precursor, provided that the culturing process provides an increase in the antibiotic-producing ability of the microorganism.
Claim 3
Dependent Claim
16 words
A method as claimed in Claim 1 in which the microorganism is Streptomyces, Cephalosporium, or Penicillium.
Claim 4
Dependent Claim
38 words
A method as claimed in Claim 1 or 2 in which the microorganism, antibiotic, antibiotic precursor, antibiotic biosynthetic pathway, and antibiotic biosynthetic gene respectively are Streptomyces, tylosin, a tylosin precursor, tylosin biosynthetic pathway, and a tylosin biosynthetic gene.
Claim 5
Dependent Claim
19 words
A method as claimed in Claim 3 in which the microorganism is S. fradiae, S. rimosus, and S. hygroscopicus.
Claim 6
Dependent Claim
24 words
A method as claimed in Claim 4 in which the tylosin biosynthetic gene is tylC, tylD, tylE, tylF, tylH, tylJ, tylK, tylL, or tylM.
Claim 7
Independent Claim
21 words
A method as claimed in any one of Claims 1 to 5 in which the biosynthetic gene is t y lF.
Claim 8
Dependent Claim
20 words
A method as claimed in Claim 1 in which the cloning vector is plasmid pHJL280, pHJL284, pHJL309, pHJL311, or pHJL315.
Claim 9
Dependent Claim
66 words
A method as claimed in claim 1 in which the microorganism transformed with a cloning vector is Streptomyces fradiae GS15/pHJL280, S. fradiae GS15/pHJL284, S. fradiae GS15/pHJL311, S. fradiae GS15/pHJL315, S. fradiae GS28/pHJL280, S. fradiae GS28/pHJL284, S. fradiae GS28/pHJL311, S. fradiae GS28/pHJL315, S. fradiae GS16/pHJL280, S. fradiae GS16/pHJL315, S. fradiae GS48/pHJL280, S. fradiae GS48/pHJL315, S. fradiae GS52/pHJL284, S. fradiae GS52/pHJL311, S. fradiae GS76/pHJL280, or S. fradiae GS76/pHJL315.
Claim 10
Dependent Claim
10 words
A recombinant DNA cloning vector as defined in claim 1.
Claim 11
Dependent Claim
7 words
Plasmids pHJL280, pHJL284, pHJL309, pHJL311, or pHJL315.
Claim 12
Dependent Claim
7 words
Plasmids pHJL280, pHJL284, pHJL309, pHJL311, or pHJL315.
Claim 13
Dependent Claim
14 words
A microorganism that is transformed with a cloning vector as claimed in claim 9.
Claim 14
Dependent Claim
11 words
A microorganism which is transformed with a plasmid of Claim 10.
Claim 15
Dependent Claim
29 words
A microorganism of Claim 12 which is E. coli K12 HB101/pHJL280, E. coli K12 HB101/pHJL284, E. coli K12 HB101/pHJL309, E. coli K12 HB101/pHJL311, or E. coli K12 JM 109/pHJL315.
Claim 16
Dependent Claim
10 words
A microorganism as claimed in Claim 12 which is Streptomyces.
Claim 17
Dependent Claim
11 words
A microorganism as claimed in Claim 14 which is Streptomyces fradiae.
Claim 18
Dependent Claim
46 words
S. fradiae GS15/pHJL280, S. fradiae GS15/pHJL284, S. fradiae GS15/pHJL311, S. fradiae GS15/pHJL335, S. fradiae GS28/gHJL280, S. fradiae GS28/pHJL284, S. fradiae GS28/pHJL311, S. fradiae GS28/pHJL315, S. fradiae GS16/pHJL315, S. fradiae. GS48/pHJL280, S. fradiae GS48/pHJL315, S. fradiae GS52/pHJL284, S. fradiae GS52/pHJL311, S. fradiae GS76/pHJL280, or S. fradiae GS76/pHJL315.
Claim 19
Dependent Claim
46 words
S. fradiae GS15/pHJL280, S. fradiae GS15/pHJL284, S. fradiae GS15/pHJL311, S. fradiae GS15/pHJL335, S. fradiae GS28/gHJL280, S. fradiae GS28/pHJL284, S. fradiae GS28/pHJL311, S. fradiae GS28/pHJL315, S. fradiae GS16/pHJL315, S. fradiae. GS48/pHJL280, S. fradiae GS48/pHJL315, S. fradiae GS52/pHJL284, S. fradiae GS52/pHJL311, S. fradiae GS76/pHJL280, or S. fradiae GS76/pHJL315.
Claim 20
Dependent Claim
23 words
A DNA sequence that encodes a gene for the t y lc, tylD, tylE, tylF, tylH, tylJ, tylK, tylL, or tylM biosynthetic genes.
Claim 21
Dependent Claim
23 words
A DNA sequence that encodes a gene for the t y lc, tylD, tylE, tylF, tylH, tylJ, tylK, tylL, or tylM biosynthetic genes.
Claim 22
Dependent Claim
24 words
A DNA sequence which encodes the promoter and translational-activating sequence of the tylC, tylD, tylE, tylF, tylH, tylJ, tylK, tylL, or tylM biosynthetic genes.
Claim 23
Dependent Claim
24 words
A DNA sequence which encodes the promoter and translational-activating sequence of the tylC, tylD, tylE, tylF, tylH, tylJ, tylK, tylL, or tylM biosynthetic genes.
Claim 24
Dependent Claim
14 words
A DNA sequence which encodes the promoter and translational-activating sequence of the tylF gene.
Claim 25
Dependent Claim
14 words
A DNA sequence which encodes the promoter and translational-activating sequence of the tylF gene.
Claim 26
Dependent Claim
74 words
A DNA sequence as claimed in Claim 19 encoding the tylF promoter that is wherein A is a deoxyadenyl residue; G is a deoxyguanyl residue; C is a deoxycytidyl residue; and T is a thymidyl residue and wherein R is a sequence of deoxyribonucleotide that is complementary to said DNA sequence depicted such that A is paired with T; T is paired with A; G is paired with C; andC is paired with G.
Claim 27
Dependent Claim
112 words
A process for preparing an antibiotic, an antibiotic precursor, or a pharmaceutically acceptable salt thereof, which comprises culturing a microorganism which produces an antibiotic or antibiotic precursor through an antibiotic biosynthetic pathway, said microorganism being transformed with a DNA cloning vector, or portion thereof, in a culture medium containing assimilable sources of carbon, nitrogen and inorganic salts under aerobic fermentation conditions characterized in that the DNA cloning vector, or portion thereof, comprises an antibiotic biosynthetic gene which codes for the expression of a rate-limiting enzyme or gene product of the antibiotic biosynthetic pathway, said antibiotic biosynthetic gene.being expressed under fermentation conditions providing for an increase in the antibiotic-producing ability of the microorganism.
Claim 28
Dependent Claim
112 words
A process for preparing an antibiotic, an antibiotic precursor, or a pharmaceutically acceptable salt thereof, which comprises culturing a microorganism which produces an antibiotic or antibiotic precursor through an antibiotic biosynthetic pathway, said microorganism being transformed with a DNA cloning vector, or portion thereof, in a culture medium containing assimilable sources of carbon, nitrogen and inorganic salts under aerobic fermentation conditions characterized in that the DNA cloning vector, or portion thereof, comprises an antibiotic biosynthetic gene which codes for the expression of a rate-limiting enzyme or gene product of the antibiotic biosynthetic pathway, said antibiotic biosynthetic gene.being expressed under fermentation conditions providing for an increase in the antibiotic-producing ability of the microorganism.
Claim 29
Dependent Claim
18 words
A process as claimed in Claim 21 in which the antibiotic or antibiotic-precursor is tylosin or a tylosin-precursor.
Claim 30
Dependent Claim
7 words
A DNA sequence encoding the tylF gene.
Claim 31
Dependent Claim
7 words
A DNA sequence encoding the tylF gene.
Claim 32
Dependent Claim
3 words
The tylF gene.
Claim 33
Dependent Claim
3 words
The tylF gene.
Claim 34
Dependent Claim
30 words
The gene claimed in claim 24 having the sequence: wherein A is deoxyadenyl residue; G is a deoxyquanyl residue; C is a deoxycytidyl residue; and T is a deoxythymidyl residue.
Claim 35
Dependent Claim
18 words
The amino acid sequence encoded by nucleotides 541 to 1371 of the tylF gene defined in claim 25.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.