Human basic fibroblast growth factor

Claim 2

A human basic fibroblast growth actor which is a polypeptide including the amino acid sequence: Pro-Ala-Leu-Pro-Glu-Asp-Gly-­Gly-Ser-Gly-Ala-Phe-Pro-Pro-­Gly-His-Phe-Lys-Asp-Pro-Lys-­Arg-Leu-Tyr-Cys-Lys-Asn-Gly-­Gly-Phe-Phe-Leu-Arg-Ile-His-­Pro-Asp-Gly-Arg-Val-Asp-Gly-­Val-Arg-Glu-Lys-Ser-Asp-Pro-­His-Ile-Lys-Leu-Gln-Leu-Gln-­Ala-Glu-Glu-Arg-Gly-Val-Val-­Ser-Ile-Lys-Gly-Val-Cys-Ala-­Asn-Arg-Try-Leu-Ala-Met-Lys-­Glu-Asp-Gly-Arg-Leu-Leu-Ala-­Ser-Lys-Cys-Val-Thr-Asp-Glu-­Cys-Phe-Phe-Phe-Glu-Arg-Leu-­Glu-Ser-Asn-Asn-Tyr-Asn-Thr-­Tyr-Arg-Ser-Arg-Lys-Tyr-Thr -­Ser-Trp-Tyr-Val-Ala-Leu-Lys-­Arg-Thr-Gly-Gln-Tyr-Lys-Leu-­Gly-Ser-Lys-Thr-Gly-Pro-Gly-­Gln-Lys-Ala-Ile-Leu-Phe-Leu-­Pro-Met-Ser-Ala-Lys-Ser

Claim 3

A recombinant DNA, which contains a base sequence coding for the human basic fibroblast growth factor claimed in Claim l.

Claim 4

The DNA as claimed in Claim 2, wherein said base sequence includes the base sequence : C C C G C C T T G C C C G A G G A T G G C G G C A G C G G C G C C T T C C C G C C C G G C C A C T T C A A G G A C C C C A A G C G G C T G T A C T G C A A A A A C G G G G G C T T C T T C C T G C G C A T C C A C C C C G A C G G C C G A G T T G A C G G G G T C C G G G A G A A G A G C G A C C C T C A C A T C A A G C T A C A A C T T C A A G C A G A A G A G A G A G G A G T T G T G T C T A T C A A A G G A G T G T G T G C T A A C C G T T A C C T G G C T A T G A A G G A A G A T G G A A G A T T A C T G G C T T C T A A A T G T G T T A C G G A T G A G T G T T T C T T T T T T G A A C G A T T G G A A T C T A A T A A C T A C A A T A C T T A C C G G T C A A G G A A A T A C A C C A G T T G G T A T G T G G C A C T G A A A C G A A C T G G G C A G T A T A A A C T T G G A T C C A A A A C A G G A C C T G G G C A G A A A G C T A T A C T T T T T C T T C C A A T G T C T G C T A A G A G C

Claim 5

A transformant as transformed as a host cell with a vector including the DNA claimed in Claim 2.

Claim 6

The transformant as claimed in Claim 4, wherein the DNA is one claimed in Claim 3.

Claim 7

A method for producing a transformant, which comprises subjecting a host cell to transformation with a vector including the DNA claimed in Claim 2.

Claim 8

A method for producing the human basic fibroblast growth factor claimed in Claim l, which comprises cultivating the transformant claimed in Claim 4 in a medium and recovering the factor as produced and accumulated in the cultured broth.

Claim 9

A substantially pure human basic fibroblast growth factor (hbFGF) protein, the hbFGF being obtained by a genetic engineering technique.

Claim 10

A substantially pure human basic fibroblast growth factor (hbFGF) protein, the hbFGF being obtained by a genetic engineering technique.

Claim 11

The human basic fibroblast growth factor (hbFGF) protein as claimed in Claim 8, wherein the content of the hbFGF protein is not less than 95% (w/w).

Claim 12

l0. A method for producing a substantially pure human basic fibroblast growth factor (hbFGF) protein, which comprises subjecting a hbFGF which is obtained by a genetic engineering technique to a purification procedure of high performance liquid chromatography using a heparin column.

Claim 13

l0. A method for producing a substantially pure human basic fibroblast growth factor (hbFGF) protein, which comprises subjecting a hbFGF which is obtained by a genetic engineering technique to a purification procedure of high performance liquid chromatography using a heparin column.